{"id":16863,"date":"2024-09-29T09:29:35","date_gmt":"2024-09-29T07:29:35","guid":{"rendered":"https:\/\/inmuno.es\/index.php\/2024\/09\/29\/spatially-resolved-whole-transcriptomic-and-proteomic-profiling-of-lung-cancer-and-its-immune-microenvironment-according-to-pd-l1-expression\/"},"modified":"2024-09-29T09:29:35","modified_gmt":"2024-09-29T07:29:35","slug":"spatially-resolved-whole-transcriptomic-and-proteomic-profiling-of-lung-cancer-and-its-immune-microenvironment-according-to-pd-l1-expression","status":"publish","type":"post","link":"https:\/\/inmuno.es\/index.php\/2024\/09\/29\/spatially-resolved-whole-transcriptomic-and-proteomic-profiling-of-lung-cancer-and-its-immune-microenvironment-according-to-pd-l1-expression\/","title":{"rendered":"Spatially resolved whole-transcriptomic and proteomic profiling of lung cancer and its immune-microenvironment according to PD-L1 expression"},"content":{"rendered":"<div>\n<p>Cancer Immunol Res. 2024 Sep 5. doi: 10.1158\/2326-6066.CIR-24-0071. Online ahead of print.<\/p>\n<p>ABSTRACT<\/p>\n<p>The expression of PD-L1 on tumor cells (TCs) is used as an immunotherapy biomarker in lung cancer, but heterogeneous intratumoral expression is often observed. Using a Digital Spatial Profiling, we performed proteomic and whole-transcriptomic analyses of TCs and immune cells (ICs) in spatially matched areas based on tumor PD-L1 expression and the status of the immune microenvironment. Our findings were validated using immunohistochemistry, The Cancer Genome Atlas, and immunotherapy cohorts. ICs in areas with high PD-L1 expression on TCs showed more features indicative of immunosuppression and exhaustion than ICs in areas with low PD-L1 expression on TCs. TCs highly expressing PD-L1 within immune-inflamed (IF) areas show up-regulation of pro-inflammatory processes, whereas TCs highly expressing PD-L1 within immune-deficient (ID) areas show up-regulation of various metabolic processes. Using differentially expressed genes of TCs between the IF and ID areas, we identified a novel prognostic gene signature for lung cancer. In addition, a high ratio of CD8+ cells to M2 macrophages was found to predict favorable outcomes in patients with PD-L1-expressing lung cancer after immune checkpoint inhibitor therapy. This study demonstrates that TCs and ICs have distinct spatial features within the tumor microenvironment that are related to tumor PD-L1 expression and IC infiltration.<\/p>\n<p>PMID:<a href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/39235761\/?utm_source=Chrome&amp;utm_medium=rss&amp;utm_content=101614637&amp;ff=20240929032735&amp;v=2.18.0.post9+e462414\">39235761<\/a> | DOI:<a href=\"https:\/\/doi.org\/10.1158\/2326-6066.CIR-24-0071\">10.1158\/2326-6066.CIR-24-0071<\/a><\/p>\n<\/div>","protected":false},"excerpt":{"rendered":"<p>Cancer Immunol Res. 2024 Sep 5. doi: 10.1158\/2326-6066.CIR-24-0071. Online ahead of print. ABSTRACT The expression of PD-L1 on tumor cells (TCs) is used as an immunotherapy biomarker in lung cancer, but heterogeneous intratumoral expression is often observed. Using a Digital Spatial Profiling, we performed proteomic and whole-transcriptomic analyses of TCs and immune cells (ICs) in &#8230; <a title=\"Spatially resolved whole-transcriptomic and proteomic profiling of lung cancer and its immune-microenvironment according to PD-L1 expression\" class=\"read-more\" href=\"https:\/\/inmuno.es\/index.php\/2024\/09\/29\/spatially-resolved-whole-transcriptomic-and-proteomic-profiling-of-lung-cancer-and-its-immune-microenvironment-according-to-pd-l1-expression\/\" aria-label=\"Read more about Spatially resolved whole-transcriptomic and proteomic profiling of lung cancer and its immune-microenvironment according to PD-L1 expression\">Read more<\/a><\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[55,42],"tags":[],"class_list":["post-16863","post","type-post","status-publish","format-standard","hentry","category-cancer-immunology-reserch","category-publicaciones"],"_links":{"self":[{"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/posts\/16863","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/comments?post=16863"}],"version-history":[{"count":0,"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/posts\/16863\/revisions"}],"wp:attachment":[{"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/media?parent=16863"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/categories?post=16863"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/tags?post=16863"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}