{"id":16920,"date":"2024-09-29T10:12:39","date_gmt":"2024-09-29T08:12:39","guid":{"rendered":"https:\/\/inmuno.es\/index.php\/2024\/09\/29\/microrna-142-regulates-gut-associated-lymphoid-tissues-and-group-3-innate-lymphoid-cells-luke-b-roberts\/"},"modified":"2024-09-29T10:12:39","modified_gmt":"2024-09-29T08:12:39","slug":"microrna-142-regulates-gut-associated-lymphoid-tissues-and-group-3-innate-lymphoid-cells-luke-b-roberts","status":"publish","type":"post","link":"https:\/\/inmuno.es\/index.php\/2024\/09\/29\/microrna-142-regulates-gut-associated-lymphoid-tissues-and-group-3-innate-lymphoid-cells-luke-b-roberts\/","title":{"rendered":"MicroRNA-142 regulates gut associated lymphoid tissues and group 3 innate lymphoid cells. Luke B Roberts"},"content":{"rendered":"<div>\n<p>Mucosal Immunol. 2024 Sep 7:S1933-0219(24)00094-1. doi: 10.1016\/j.mucimm.2024.09.001. Online ahead of print.<\/p>\n<p>ABSTRACT<\/p>\n<p>The transcriptomic signatures that shape responses of innate lymphoid cells (ILCs) have been well characterised, however post-transcriptional mechanisms which regulate their development and activity remain poorly understood. We demonstrate that ILC groups of the intestinal lamina propria express mature forms of microRNA-142 (miR-142), an evolutionarily conserved microRNA family with several non-redundant regulatory roles within the immune system. Germline Mir142 deletion alters intestinal ILC compositions, resulting in the absence of T-bet+ populations and significant defects in the cellularity and phenotypes of ILC3 subsets including CCR6+ LTi-like ILC3s. These effects were associated with decreased pathology in an innate-immune cell driven model of colitis. Furthermore, Mir142-\/- mice demonstrate defective development of gut-associated lymphoid tissues, including a complete absence of mature Peyer&#8217;s patches. Conditional deletion of Mir142 in ILC3s (Rorc\u0394Mir142) supported cell-intrinsic roles for these microRNAs in establishing or maintaining cellularity and functions of LTi-like ILC3s in intestinal associated tissues. RNAseq analysis revealed several target genes and biological pathways potentially regulated by miR-142 microRNAs in these cells. Finally, lack of Mir142 in ILC3 led to elevated IL-17A production. These data broaden our understanding of immune system roles of miR-142 microRNAs, identifying these molecules as critical post-transcriptional regulators of ILC3s and intestinal mucosal immunity.<\/p>\n<p>PMID:<a href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/39245145\/?utm_source=Chrome&amp;utm_medium=rss&amp;utm_content=101299742&amp;ff=20240929041234&amp;v=2.18.0.post9+e462414\">39245145<\/a> | DOI:<a href=\"https:\/\/doi.org\/10.1016\/j.mucimm.2024.09.001\">10.1016\/j.mucimm.2024.09.001<\/a><\/p>\n<\/div>","protected":false},"excerpt":{"rendered":"<p>Mucosal Immunol. 2024 Sep 7:S1933-0219(24)00094-1. doi: 10.1016\/j.mucimm.2024.09.001. Online ahead of print. ABSTRACT The transcriptomic signatures that shape responses of innate lymphoid cells (ILCs) have been well characterised, however post-transcriptional mechanisms which regulate their development and activity remain poorly understood. We demonstrate that ILC groups of the intestinal lamina propria express mature forms of microRNA-142 (miR-142), &#8230; <a title=\"MicroRNA-142 regulates gut associated lymphoid tissues and group 3 innate lymphoid cells. Luke B Roberts\" class=\"read-more\" href=\"https:\/\/inmuno.es\/index.php\/2024\/09\/29\/microrna-142-regulates-gut-associated-lymphoid-tissues-and-group-3-innate-lymphoid-cells-luke-b-roberts\/\" aria-label=\"Read more about MicroRNA-142 regulates gut associated lymphoid tissues and group 3 innate lymphoid cells. Luke B Roberts\">Read more<\/a><\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[57,42],"tags":[],"class_list":["post-16920","post","type-post","status-publish","format-standard","hentry","category-mucosal-immunology","category-publicaciones"],"_links":{"self":[{"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/posts\/16920","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/comments?post=16920"}],"version-history":[{"count":0,"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/posts\/16920\/revisions"}],"wp:attachment":[{"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/media?parent=16920"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/categories?post=16920"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/tags?post=16920"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}