{"id":17187,"date":"2024-10-05T12:14:09","date_gmt":"2024-10-05T10:14:09","guid":{"rendered":"https:\/\/inmuno.es\/index.php\/2024\/10\/05\/the-interaction-of-galectin-8-c-terminal-domain-with-cell-surface-glycoconjugates-modulates-membrane-elasticity-to-stimulate-antigen-uptake-and-presentation-to-cd4-t-cellscecilia-arahi-prato\/"},"modified":"2024-10-05T12:14:09","modified_gmt":"2024-10-05T10:14:09","slug":"the-interaction-of-galectin-8-c-terminal-domain-with-cell-surface-glycoconjugates-modulates-membrane-elasticity-to-stimulate-antigen-uptake-and-presentation-to-cd4-t-cellscecilia-arahi-prato","status":"publish","type":"post","link":"https:\/\/inmuno.es\/index.php\/2024\/10\/05\/the-interaction-of-galectin-8-c-terminal-domain-with-cell-surface-glycoconjugates-modulates-membrane-elasticity-to-stimulate-antigen-uptake-and-presentation-to-cd4-t-cellscecilia-arahi-prato\/","title":{"rendered":"The interaction of galectin-8 C-terminal domain with cell surface glycoconjugates modulates membrane elasticity to stimulate antigen uptake and presentation to CD4 T cells\u200bCecilia Arahi Prato on 4 de October de 2024 at 10:00"},"content":{"rendered":"
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J Leukoc Biol. 2024 Oct 4:qiae214. doi: 10.1093\/jleuko\/qiae214. Online ahead of print.<\/p>\n

ABSTRACT<\/p>\n

Galectins constitute a family of soluble lectins with unique capacity to induce macroscale rearrangements upon interacting with cell membrane glycoconjugates. Galectin-8 (Gal-8) is acknowledged for its role in facilitating antigen uptake and processing upon engaging with cell surface glycoconjugates on antigen-presenting cells (APCs). Gal-8 consists of two covalently fused N- and C-terminal carbohydrate recognition domains (N- and C-CRD), each exhibiting distinct glycan specificity. In this study, we utilized single N- and C-CRD recombinant proteins to dissect the nature of Gal-8-glycan interactions during antigen internalization enhancement. Single C-CRD was able to replicate the effect of full-length Gal-8 (FLGal-8) on antigen internalization in BMDCs. Antigen uptake enhancement was diminished in the presence of lactose or when N-glycosylation-deficient macrophages served as APCs, underscoring the significance of glycan recognition. Measurement of the elastic modulus using Atomic Force Microscopy unveiled that FLGal-8- and C-CRD-stimulated macrophages exhibited heightened membrane stiffness compared to untreated cells, providing a plausible mechanism for their involvement in endocytosis. C-CRD proved to be as efficient as FLGal-8 in promoting antigen degradation, suggesting its implication in antigen-processing induction. Lastly, C-CRD was able to replicate FLGal-8-induced antigen presentation in the MHC-II context both in vitro and in vivo. Our findings support the notion that Gal-8 binds through its C-CRD to cell surface N-glycans, thereby altering membrane mechanical forces conducive to soluble antigen endocytosis, processing, and presentation to cognate CD4 T-cells. These findings contribute to a deeper comprehension of Gal-8 and its mechanisms of action, paving the way for the development of more efficacious immunotherapies.<\/p>\n

PMID:39365278<\/a> | DOI:10.1093\/jleuko\/qiae214<\/a><\/p>\n<\/div>","protected":false},"excerpt":{"rendered":"

J Leukoc Biol. 2024 Oct 4:qiae214. doi: 10.1093\/jleuko\/qiae214. Online ahead of print. ABSTRACT Galectins constitute a family of soluble lectins with unique capacity to induce macroscale rearrangements upon interacting with cell membrane glycoconjugates. Galectin-8 (Gal-8) is acknowledged for its role in facilitating antigen uptake and processing upon engaging with cell surface glycoconjugates on antigen-presenting cells … Read more<\/a><\/p>\n","protected":false},"author":0,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[86,42],"tags":[],"class_list":["post-17187","post","type-post","status-publish","format-standard","hentry","category-journal-of-leukocyte-biology","category-publicaciones"],"_links":{"self":[{"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/posts\/17187","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/types\/post"}],"replies":[{"embeddable":true,"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/comments?post=17187"}],"version-history":[{"count":0,"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/posts\/17187\/revisions"}],"wp:attachment":[{"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/media?parent=17187"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/categories?post=17187"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/tags?post=17187"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}