{"id":17191,"date":"2024-10-05T15:53:20","date_gmt":"2024-10-05T13:53:20","guid":{"rendered":"https:\/\/inmuno.es\/index.php\/2024\/10\/05\/highly-sensitive-reporter-cell-line-for-detection-of-interferon-types-i-iii-and-their-neutralization-by-antibodies-valuekevin-groen-roger-kuratli-lauren-sar-andri-vasou-mich\/"},"modified":"2024-10-05T15:53:20","modified_gmt":"2024-10-05T13:53:20","slug":"highly-sensitive-reporter-cell-line-for-detection-of-interferon-types-i-iii-and-their-neutralization-by-antibodies-valuekevin-groen-roger-kuratli-lauren-sar-andri-vasou-mich","status":"publish","type":"post","link":"https:\/\/inmuno.es\/index.php\/2024\/10\/05\/highly-sensitive-reporter-cell-line-for-detection-of-interferon-types-i-iii-and-their-neutralization-by-antibodies-valuekevin-groen-roger-kuratli-lauren-sar-andri-vasou-mich\/","title":{"rendered":"Highly sensitive reporter cell line for detection of interferon types I\u2013III and their neutralization by antibodies. [[{&#8220;value&#8221;:&#8221;Kevin Groen, \nRoger Kuratli, \nLauren Sar, \nAndri Vasou, \nMichael Huber, \nDavid J. Hughes, \nBenjamin G. Hale&#8221;}]]"},"content":{"rendered":"<p>Interferons (IFNs) are a critical component of innate immune defenses and limit viral disease severity. To advance studies on IFNs and their neutralization by pathogenic autoantibodies, we generated a Renilla luciferase-based reporter cell line capable of detecting the activities of IFN-Is, IFN-II, and IFN-IIIs. The reporter cell line exhibits a 125- to 2000-fold higher sensitivity to IFNs than a commonly used alternative biological reporter system and allows for a rapid and simple live-cell workflow for detecting low titer amounts of neutralizing anti-IFN antibodies.<\/p>\n<p><\/p>","protected":false},"excerpt":{"rendered":"<p>Interferons (IFNs) are a critical component of innate immune defenses and limit viral disease severity. To advance studies on IFNs and their neutralization by pathogenic autoantibodies, we generated a Renilla luciferase-based reporter cell line capable of detecting the activities of IFN-Is, IFN-II, and IFN-IIIs. The reporter cell line exhibits a 125- to 2000-fold higher sensitivity &#8230; <a title=\"Highly sensitive reporter cell line for detection of interferon types I\u2013III and their neutralization by antibodies. [[{&#8220;value&#8221;:&#8221;Kevin Groen, \nRoger Kuratli, \nLauren Sar, \nAndri Vasou, \nMichael Huber, \nDavid J. Hughes, \nBenjamin G. Hale&#8221;}]]\" class=\"read-more\" href=\"https:\/\/inmuno.es\/index.php\/2024\/10\/05\/highly-sensitive-reporter-cell-line-for-detection-of-interferon-types-i-iii-and-their-neutralization-by-antibodies-valuekevin-groen-roger-kuratli-lauren-sar-andri-vasou-mich\/\" aria-label=\"Read more about Highly sensitive reporter cell line for detection of interferon types I\u2013III and their neutralization by antibodies. [[{&#8220;value&#8221;:&#8221;Kevin Groen, \nRoger Kuratli, \nLauren Sar, \nAndri Vasou, \nMichael Huber, \nDavid J. Hughes, \nBenjamin G. Hale&#8221;}]]\">Read more<\/a><\/p>\n","protected":false},"author":0,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[112,42],"tags":[],"class_list":["post-17191","post","type-post","status-publish","format-standard","hentry","category-european-journal-of-immunology","category-publicaciones"],"_links":{"self":[{"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/posts\/17191","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/types\/post"}],"replies":[{"embeddable":true,"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/comments?post=17191"}],"version-history":[{"count":0,"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/posts\/17191\/revisions"}],"wp:attachment":[{"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/media?parent=17191"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/categories?post=17191"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/tags?post=17191"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}