{"id":17285,"date":"2024-10-08T04:47:42","date_gmt":"2024-10-08T02:47:42","guid":{"rendered":"https:\/\/inmuno.es\/index.php\/2024\/10\/08\/in-vivo-crispr-screening-reveals-chd7-as-a-positive-regulator-of-short-lived-effector-cells\/"},"modified":"2024-10-08T04:47:42","modified_gmt":"2024-10-08T02:47:42","slug":"in-vivo-crispr-screening-reveals-chd7-as-a-positive-regulator-of-short-lived-effector-cells","status":"publish","type":"post","link":"https:\/\/inmuno.es\/index.php\/2024\/10\/08\/in-vivo-crispr-screening-reveals-chd7-as-a-positive-regulator-of-short-lived-effector-cells\/","title":{"rendered":"In\u00a0Vivo CRISPR Screening Reveals CHD7 as a Positive Regulator of Short-lived Effector Cells"},"content":{"rendered":"<div>\n<p>J Immunol. 2024 Oct 7:ji2400213. doi: 10.4049\/jimmunol.2400213. Online ahead of print.<\/p>\n<p>ABSTRACT<\/p>\n<p>CD8+ T cells differentiate into two subpopulations in response to acute viral infection: memory precursor effector cells (MPECs) and short-lived effector cells (SLECs). MPECs and SLECs are epigenetically distinct; however, the epigenetic regulators required for formation of these subpopulations are mostly unknown. In this study, we performed an in vivo CRISPR screen in murine naive CD8+ T cells to identify the epigenetic regulators required for MPEC and SLEC formation, using the acute lymphocytic choriomeningitis virus Armstrong infection model. We identified the ATP-dependent chromatin remodeler CHD7 (chromodomain-helicase DNA-binding protein 7) as a positive regulator of SLEC formation, as knockout (KO) of Chd7 reduced SLECs numerically. In contrast, KO of Chd7 increased the formation of central memory T cells following pathogen clearance yet attenuated memory cell expansion following a rechallenge. These findings establish CHD7 as a novel positive regulator of SLEC and a negative regulator of central memory T cell formation.<\/p>\n<p>PMID:<a href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/39373572\/?utm_source=WordPress&amp;utm_medium=rss&amp;utm_content=2985117R&amp;ff=20241007224741&amp;v=2.18.0.post9+e462414\">39373572<\/a> | DOI:<a href=\"https:\/\/doi.org\/10.4049\/jimmunol.2400213\">10.4049\/jimmunol.2400213<\/a><\/p>\n<\/div>","protected":false},"excerpt":{"rendered":"<p>J Immunol. 2024 Oct 7:ji2400213. doi: 10.4049\/jimmunol.2400213. Online ahead of print. ABSTRACT CD8+ T cells differentiate into two subpopulations in response to acute viral infection: memory precursor effector cells (MPECs) and short-lived effector cells (SLECs). MPECs and SLECs are epigenetically distinct; however, the epigenetic regulators required for formation of these subpopulations are mostly unknown. In &#8230; <a title=\"In\u00a0Vivo CRISPR Screening Reveals CHD7 as a Positive Regulator of Short-lived Effector Cells\" class=\"read-more\" href=\"https:\/\/inmuno.es\/index.php\/2024\/10\/08\/in-vivo-crispr-screening-reveals-chd7-as-a-positive-regulator-of-short-lived-effector-cells\/\" aria-label=\"Read more about In\u00a0Vivo CRISPR Screening Reveals CHD7 as a Positive Regulator of Short-lived Effector Cells\">Read more<\/a><\/p>\n","protected":false},"author":0,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[42,71],"tags":[],"class_list":["post-17285","post","type-post","status-publish","format-standard","hentry","category-publicaciones","category-the-journal-of-immunology"],"_links":{"self":[{"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/posts\/17285","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/types\/post"}],"replies":[{"embeddable":true,"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/comments?post=17285"}],"version-history":[{"count":0,"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/posts\/17285\/revisions"}],"wp:attachment":[{"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/media?parent=17285"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/categories?post=17285"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/tags?post=17285"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}