{"id":18776,"date":"2024-10-25T21:49:36","date_gmt":"2024-10-25T19:49:36","guid":{"rendered":"https:\/\/inmuno.es\/index.php\/2024\/10\/25\/reversible-role-of-mir654-3p-and-mir9-3p-in-pathogenesis-of-epstein-barr-virus-negative-but-not-positive-burkitts-lymphomayu-gong-on-24-de-october-de-2024-at-1000\/"},"modified":"2024-10-25T21:49:36","modified_gmt":"2024-10-25T19:49:36","slug":"reversible-role-of-mir654-3p-and-mir9-3p-in-pathogenesis-of-epstein-barr-virus-negative-but-not-positive-burkitts-lymphomayu-gong-on-24-de-october-de-2024-at-1000","status":"publish","type":"post","link":"https:\/\/inmuno.es\/index.php\/2024\/10\/25\/reversible-role-of-mir654-3p-and-mir9-3p-in-pathogenesis-of-epstein-barr-virus-negative-but-not-positive-burkitts-lymphomayu-gong-on-24-de-october-de-2024-at-1000\/","title":{"rendered":"Reversible role of MIR654\/3P and MIR9\/3P in pathogenesis of Epstein-Barr Virus negative, but not positive, Burkitt&#8217;s lymphoma\u200bYu Gong   on 24 de October de 2024 at 10:00"},"content":{"rendered":"<div>\n<p>J Leukoc Biol. 2024 Oct 24:qiae237. doi: 10.1093\/jleuko\/qiae237. Online ahead of print.<\/p>\n<p>ABSTRACT<\/p>\n<p>The role of MIR654 in Burkitt&#8217;s lymphoma (BL) and whether it impacts expression of MYC, and its downstream activated MIR9 is not known. Expression of MYC, MYCN, MYCL, MIR9\/3P, MIR654\/5P, and MIR654\/3P were assessed by qRT-PCR in biopsy samples from Epstein-Barr virus (EBV)- and EBV+ BL patients and BL cell lines. Effects of modulation of MIR9\/3P and MIR654\/3P on cell proliferation, apoptosis and chemosensitivity were evaluated. Luciferase reporter assay was performed to validate putative target of MIR654\/5P. Effects of MIR9\/3P and MIR654\/3P on tumor burden and disease outcome were evaluated using xenograft model of BL. Expression of MYC, MYCN, and MIR9\/3P was higher in all BL patient samples and cell lines. Expression of MIR654\/3P was downregulated in EBV- BL patient samples and cell lines compared to either noncancer lymphoid reactive hyperplasia (LRH) or EBV+ samples and cell lines. Additionally, MIR654\/3P overexpression inhibited cell proliferation, induced apoptosis, and increased chemosensitivity in EBV- BL cell lines. Luciferase reporter assay confirmed that MYC is a target of MIR654\/3P in both EBV- and EBV+ BL cell lines; however, the effect of MIR654\/3P-mediated targeting of MYC is overridden in EBV+ cells. Administration of MIR654\/3P mimic or MIR9\/3P antagomir in the xenograft model decreased tumor burden and increased survival. Combined intervention with MIR654\/3P mimic and MIR9\/3P antagomir had synergistic action on decreasing tumor burden and improving disease outcome. MIR654\/3P, as a putative tumor suppressor in EBV- BL, collaborated MIR9\/3P might serve as a therapeutic agent to treat EBV- BL patients in combination with existing chemotherapy and immunotherapy regimes.<\/p>\n<p>PMID:<a href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/39446559\/?utm_source=WordPress&amp;utm_medium=rss&amp;utm_content=8405628&amp;ff=20241025154936&amp;v=2.18.0.post9+e462414\">39446559<\/a> | DOI:<a href=\"https:\/\/doi.org\/10.1093\/jleuko\/qiae237\">10.1093\/jleuko\/qiae237<\/a><\/p>\n<\/div>","protected":false},"excerpt":{"rendered":"<p>J Leukoc Biol. 2024 Oct 24:qiae237. doi: 10.1093\/jleuko\/qiae237. Online ahead of print. ABSTRACT The role of MIR654 in Burkitt&#8217;s lymphoma (BL) and whether it impacts expression of MYC, and its downstream activated MIR9 is not known. Expression of MYC, MYCN, MYCL, MIR9\/3P, MIR654\/5P, and MIR654\/3P were assessed by qRT-PCR in biopsy samples from Epstein-Barr virus &#8230; <a title=\"Reversible role of MIR654\/3P and MIR9\/3P in pathogenesis of Epstein-Barr Virus negative, but not positive, Burkitt&#8217;s lymphoma\u200bYu Gong   on 24 de October de 2024 at 10:00\" class=\"read-more\" href=\"https:\/\/inmuno.es\/index.php\/2024\/10\/25\/reversible-role-of-mir654-3p-and-mir9-3p-in-pathogenesis-of-epstein-barr-virus-negative-but-not-positive-burkitts-lymphomayu-gong-on-24-de-october-de-2024-at-1000\/\" aria-label=\"Read more about Reversible role of MIR654\/3P and MIR9\/3P in pathogenesis of Epstein-Barr Virus negative, but not positive, Burkitt&#8217;s lymphoma\u200bYu Gong   on 24 de October de 2024 at 10:00\">Read more<\/a><\/p>\n","protected":false},"author":0,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[86,42],"tags":[],"class_list":["post-18776","post","type-post","status-publish","format-standard","hentry","category-journal-of-leukocyte-biology","category-publicaciones"],"_links":{"self":[{"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/posts\/18776","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/types\/post"}],"replies":[{"embeddable":true,"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/comments?post=18776"}],"version-history":[{"count":0,"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/posts\/18776\/revisions"}],"wp:attachment":[{"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/media?parent=18776"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/categories?post=18776"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/tags?post=18776"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}