{"id":19856,"date":"2024-12-21T10:16:32","date_gmt":"2024-12-21T09:16:32","guid":{"rendered":"https:\/\/inmuno.es\/index.php\/2024\/12\/21\/b-cell-receptor-repertoire-analysis-of-the-cd21lo-b-cell-compartment-in-healthy-individuals-patients-with-sjogrens-disease-and-patients-with-radiographic-axial-spondyloarthritis-valueri\/"},"modified":"2024-12-21T10:16:32","modified_gmt":"2024-12-21T09:16:32","slug":"b-cell-receptor-repertoire-analysis-of-the-cd21lo-b-cell-compartment-in-healthy-individuals-patients-with-sjogrens-disease-and-patients-with-radiographic-axial-spondyloarthritis-valueri","status":"publish","type":"post","link":"https:\/\/inmuno.es\/index.php\/2024\/12\/21\/b-cell-receptor-repertoire-analysis-of-the-cd21lo-b-cell-compartment-in-healthy-individuals-patients-with-sjogrens-disease-and-patients-with-radiographic-axial-spondyloarthritis-valueri\/","title":{"rendered":"B Cell Receptor Repertoire Analysis of the CD21lo B Cell Compartment in Healthy Individuals, Patients With Sj\u00f6gren&#8217;s Disease, and Patients With Radiographic Axial Spondyloarthritis. [[{&#8220;value&#8221;:&#8221;Rick Wilbrink, \nLinda van der Weele, \nAnneke J. P. L. Spoorenberg, \nNiek de Vries, \nIlse T. G. Niewold, \nGwenny M. Verstappen, \nFrans G. M. Kroese&#8221;}]]"},"content":{"rendered":"<p>This study demonstrates that CD27\u2212CD21lo B cells harbor a relatively low frequency of somatic mutations in their BCR compared with CD27+CD21lo B cells and early plasmablasts. Clonal relationships were present among all three subsets in both health and disease, indicating that CD27\u2212CD21lo B cells actively contribute to immune responses.<\/p>\n<p><\/p>\n<h2>ABSTRACT<\/h2>\n<p>B cells with low or absent expression of CD21 (CD21lo B cells) gained attention due to their expansion in the peripheral blood of patients with immune-mediated, rheumatic diseases. This is not only observed in typical autoimmune diseases like systemic lupus erythematosus and Sj\u00f6gren&#8217;s disease (SjD) but also in radiographic axial spondyloarthritis (r-axSpA), which is considered an autoinflammatory disease. To gain more insight into the origins of the heterogeneous CD21lo B-cell population, and its relation to the plasmablast (PB) compartment, we profiled the B-cell-receptor (BCR) repertoire in CD27\u2212 and CD27+ fractions of CD21lo B cells and early PBs using next-generation sequencing. Populations were sorted from peripheral blood of healthy individuals, SjD patients, and r-axSpA patients (n = 10 for each group). In healthy individuals and both patient groups, our findings indicate that CD27\u2212CD21lo B cells, which exhibit few mutations in their BCR, may develop into CD27+CD21lo B cells and PBs, both marked by considerably more mutations. Given the known expansion of circulating CD27\u2212CD21lo B cells in SjD and r-axSpA patients and clonal relationships with both CD27+CD21lo B cells and early PBs, these cells might actively contribute to (pathological) immune responses in rheumatic diseases with autoimmune and\/or autoinflammatory characteristics.<\/p>","protected":false},"excerpt":{"rendered":"<p>This study demonstrates that CD27\u2212CD21lo B cells harbor a relatively low frequency of somatic mutations in their BCR compared with CD27+CD21lo B cells and early plasmablasts. Clonal relationships were present among all three subsets in both health and disease, indicating that CD27\u2212CD21lo B cells actively contribute to immune responses. ABSTRACT B cells with low or &#8230; <a title=\"B Cell Receptor Repertoire Analysis of the CD21lo B Cell Compartment in Healthy Individuals, Patients With Sj\u00f6gren&#8217;s Disease, and Patients With Radiographic Axial Spondyloarthritis. [[{&#8220;value&#8221;:&#8221;Rick Wilbrink, \nLinda van der Weele, \nAnneke J. P. L. Spoorenberg, \nNiek de Vries, \nIlse T. G. Niewold, \nGwenny M. Verstappen, \nFrans G. M. Kroese&#8221;}]]\" class=\"read-more\" href=\"https:\/\/inmuno.es\/index.php\/2024\/12\/21\/b-cell-receptor-repertoire-analysis-of-the-cd21lo-b-cell-compartment-in-healthy-individuals-patients-with-sjogrens-disease-and-patients-with-radiographic-axial-spondyloarthritis-valueri\/\" aria-label=\"Read more about B Cell Receptor Repertoire Analysis of the CD21lo B Cell Compartment in Healthy Individuals, Patients With Sj\u00f6gren&#8217;s Disease, and Patients With Radiographic Axial Spondyloarthritis. [[{&#8220;value&#8221;:&#8221;Rick Wilbrink, \nLinda van der Weele, \nAnneke J. P. L. Spoorenberg, \nNiek de Vries, \nIlse T. G. Niewold, \nGwenny M. Verstappen, \nFrans G. M. Kroese&#8221;}]]\">Read more<\/a><\/p>\n","protected":false},"author":0,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[112,42],"tags":[],"class_list":["post-19856","post","type-post","status-publish","format-standard","hentry","category-european-journal-of-immunology","category-publicaciones"],"_links":{"self":[{"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/posts\/19856","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/types\/post"}],"replies":[{"embeddable":true,"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/comments?post=19856"}],"version-history":[{"count":0,"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/posts\/19856\/revisions"}],"wp:attachment":[{"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/media?parent=19856"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/categories?post=19856"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/tags?post=19856"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}