{"id":22877,"date":"2025-01-21T18:47:54","date_gmt":"2025-01-21T17:47:54","guid":{"rendered":"https:\/\/inmuno.es\/index.php\/2025\/01\/21\/single-cell-rna-sequencing-revealed-the-immunophenotypic-features-of-macrophages-in-cardiac-transplants-and-uncovered-lgals9-promoted-their-polarization-towards-the-m2b-subtypeyuexing-zhu-o\/"},"modified":"2025-01-21T18:47:54","modified_gmt":"2025-01-21T17:47:54","slug":"single-cell-rna-sequencing-revealed-the-immunophenotypic-features-of-macrophages-in-cardiac-transplants-and-uncovered-lgals9-promoted-their-polarization-towards-the-m2b-subtypeyuexing-zhu-o","status":"publish","type":"post","link":"https:\/\/inmuno.es\/index.php\/2025\/01\/21\/single-cell-rna-sequencing-revealed-the-immunophenotypic-features-of-macrophages-in-cardiac-transplants-and-uncovered-lgals9-promoted-their-polarization-towards-the-m2b-subtypeyuexing-zhu-o\/","title":{"rendered":"Single-cell RNA Sequencing Revealed the Immunophenotypic Features of Macrophages in Cardiac Transplants and Uncovered Lgals9 Promoted Their Polarization towards The M2b Subtype\u200bYuexing Zhu   on 21 de January de 2025 at 11:00"},"content":{"rendered":"<div>\n<p><b>J Leukoc Biol<\/b>. 2025 Jan 21:qiaf009. doi: 10.1093\/jleuko\/qiaf009. Online ahead of print.<\/p>\n<p><b>ABSTRACT<\/b><\/p>\n<p>Macrophages play a crucial role in the immune response during allograft rejection in organ transplantation. Therefore, our study aimed to explore the genomic features of macrophages in mouse heart transplants and use single-cell RNA sequencing to investigate Galectin-9 (Gal-9, Lgals9), a lectin that can mediate the activation and differentiation of immune cells through ligand-receptor interactions, and the effects of its regulation in transplantation. We discovered a new subset of macrophages called &#8220;Myoz2+ macrophages&#8221;, which specifically expressed genes related to myocardial contraction. We identified a distinct differentiation trajectory and process for the Saa3+ macrophage population, representing anti-inflammatory functionality. Also, we observed a significant downregulation of Lgals9 expression in the macrophages after mouse heart transplantation. Then, we validated our findings using RT-qPCR and Western blotting and also investigated the impact of Lgals9 on macrophage function through flow cytometry and ELISA. Furthermore, in vitro, we found that rLgals9 (Recombinant Mouse Galectin-9 Protein) treated macrophages polarized towards the M2b phenotype at appropriate concentrations.<\/p>\n<p>PMID:<a href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/39835675\/?utm_source=WordPress&amp;utm_medium=rss&amp;utm_content=8405628&amp;ff=20250121124754&amp;v=2.18.0.post9+e462414\">39835675<\/a> | DOI:<a href=\"https:\/\/doi.org\/10.1093\/jleuko\/qiaf009\">10.1093\/jleuko\/qiaf009<\/a><\/p>\n<\/div>","protected":false},"excerpt":{"rendered":"<p>J Leukoc Biol. 2025 Jan 21:qiaf009. doi: 10.1093\/jleuko\/qiaf009. Online ahead of print. ABSTRACT Macrophages play a crucial role in the immune response during allograft rejection in organ transplantation. Therefore, our study aimed to explore the genomic features of macrophages in mouse heart transplants and use single-cell RNA sequencing to investigate Galectin-9 (Gal-9, Lgals9), a lectin &#8230; <a title=\"Single-cell RNA Sequencing Revealed the Immunophenotypic Features of Macrophages in Cardiac Transplants and Uncovered Lgals9 Promoted Their Polarization towards The M2b Subtype\u200bYuexing Zhu   on 21 de January de 2025 at 11:00\" class=\"read-more\" href=\"https:\/\/inmuno.es\/index.php\/2025\/01\/21\/single-cell-rna-sequencing-revealed-the-immunophenotypic-features-of-macrophages-in-cardiac-transplants-and-uncovered-lgals9-promoted-their-polarization-towards-the-m2b-subtypeyuexing-zhu-o\/\" aria-label=\"Read more about Single-cell RNA Sequencing Revealed the Immunophenotypic Features of Macrophages in Cardiac Transplants and Uncovered Lgals9 Promoted Their Polarization towards The M2b Subtype\u200bYuexing Zhu   on 21 de January de 2025 at 11:00\">Read more<\/a><\/p>\n","protected":false},"author":0,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[86,42],"tags":[],"class_list":["post-22877","post","type-post","status-publish","format-standard","hentry","category-journal-of-leukocyte-biology","category-publicaciones"],"_links":{"self":[{"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/posts\/22877","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/types\/post"}],"replies":[{"embeddable":true,"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/comments?post=22877"}],"version-history":[{"count":0,"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/posts\/22877\/revisions"}],"wp:attachment":[{"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/media?parent=22877"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/categories?post=22877"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/tags?post=22877"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}