{"id":25483,"date":"2025-02-24T08:59:41","date_gmt":"2025-02-24T07:59:41","guid":{"rendered":"https:\/\/inmuno.es\/index.php\/2025\/02\/24\/interleukin-10-production-by-innate-lymphoid-cells-restricts-intestinal-inflammation-in-mice-jing-li\/"},"modified":"2025-02-24T08:59:41","modified_gmt":"2025-02-24T07:59:41","slug":"interleukin-10-production-by-innate-lymphoid-cells-restricts-intestinal-inflammation-in-mice-jing-li","status":"publish","type":"post","link":"https:\/\/inmuno.es\/index.php\/2025\/02\/24\/interleukin-10-production-by-innate-lymphoid-cells-restricts-intestinal-inflammation-in-mice-jing-li\/","title":{"rendered":"Interleukin-10 production by innate lymphoid cells restricts intestinal inflammation in mice. Jing Li"},"content":{"rendered":"<div>\n<p><b>Mucosal Immunol<\/b>. 2025 Feb 21:S1933-0219(25)00023-6. doi: 10.1016\/j.mucimm.2025.02.005. Online ahead of print.<\/p>\n<p><b>ABSTRACT<\/b><\/p>\n<p>Interleukin-10 (IL-10) is an immunomodulatory cytokine critical for intestinal immune homeostasis. IL-10 is produced by various immune cells but IL-10 receptor signaling in intestinal CX<sub>3<\/sub>CR1<sup>+<\/sup> mononuclear phagocytes is necessary to prevent spontaneous colitis in mice. Here, we utilized fluorescent protein reporters and cell-specific targeting and found that Rorc-expressing innate lymphoid cells (ILCs) produce IL-10 in response to anti-CD40-mediated intestinal inflammation. Deletion of Il10 specifically in Rorc-expressing ILCs led to phenotypic changes in intestinal macrophages and exacerbated both innate and adaptive immune-mediated models of experimental colitis. The population of IL-10<sup>+<\/sup> producing ILCs shared markers with both ILC2 and ILC3 with nearly all ILC3s being of NCR<sup>+<\/sup> subtype. Interestingly, Ccl26 was enriched in IL-10<sup>+<\/sup> ILCs and markedly reduced in IL-10-deficient ILC3s. Since CCL26 is a ligand for CX<sub>3<\/sub>CR1, we employed RNA in situ hybridization and observed increased numbers of ILCs in close proximity to Cx3cr1-expressing cells under inflammatory conditions. Finally, we generated a transgenic Rorc<sup>tdTomato<\/sup> reporter mouse that faithfully marked ROR\u03b3t<sup>+<\/sup> cells that could rescue disease pathology and aberrant macrophage phenotype following adoptive transfer into mice with selective Il10 deficiency in ILC3s. These results demonstrate that IL-10 production by a population of ILCs functions to promote immune homeostasis in the intestine possibly via direct effects on intestinal macrophages.<\/p>\n<p>PMID:<a href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/39988202\/?utm_source=WordPress&amp;utm_medium=rss&amp;utm_content=101299742&amp;ff=20250224025939&amp;v=2.18.0.post9+e462414\">39988202<\/a> | DOI:<a href=\"https:\/\/doi.org\/10.1016\/j.mucimm.2025.02.005\">10.1016\/j.mucimm.2025.02.005<\/a><\/p>\n<\/div>","protected":false},"excerpt":{"rendered":"<p>Mucosal Immunol. 2025 Feb 21:S1933-0219(25)00023-6. doi: 10.1016\/j.mucimm.2025.02.005. Online ahead of print. ABSTRACT Interleukin-10 (IL-10) is an immunomodulatory cytokine critical for intestinal immune homeostasis. IL-10 is produced by various immune cells but IL-10 receptor signaling in intestinal CX3CR1+ mononuclear phagocytes is necessary to prevent spontaneous colitis in mice. Here, we utilized fluorescent protein reporters and cell-specific &#8230; <a title=\"Interleukin-10 production by innate lymphoid cells restricts intestinal inflammation in mice. Jing Li\" class=\"read-more\" href=\"https:\/\/inmuno.es\/index.php\/2025\/02\/24\/interleukin-10-production-by-innate-lymphoid-cells-restricts-intestinal-inflammation-in-mice-jing-li\/\" aria-label=\"Read more about Interleukin-10 production by innate lymphoid cells restricts intestinal inflammation in mice. Jing Li\">Read more<\/a><\/p>\n","protected":false},"author":0,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[57,42],"tags":[],"class_list":["post-25483","post","type-post","status-publish","format-standard","hentry","category-mucosal-immunology","category-publicaciones"],"_links":{"self":[{"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/posts\/25483","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/types\/post"}],"replies":[{"embeddable":true,"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/comments?post=25483"}],"version-history":[{"count":0,"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/posts\/25483\/revisions"}],"wp:attachment":[{"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/media?parent=25483"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/categories?post=25483"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/tags?post=25483"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}