{"id":27118,"date":"2025-03-12T23:50:23","date_gmt":"2025-03-12T22:50:23","guid":{"rendered":"https:\/\/inmuno.es\/index.php\/2025\/03\/12\/genetic-evidence-for-the-suppressive-role-of-zebrafish-vhl-targeting-mavs-in-antiviral-innate-immunity-during-rna-virus-infection\/"},"modified":"2025-03-12T23:50:23","modified_gmt":"2025-03-12T22:50:23","slug":"genetic-evidence-for-the-suppressive-role-of-zebrafish-vhl-targeting-mavs-in-antiviral-innate-immunity-during-rna-virus-infection","status":"publish","type":"post","link":"https:\/\/inmuno.es\/index.php\/2025\/03\/12\/genetic-evidence-for-the-suppressive-role-of-zebrafish-vhl-targeting-mavs-in-antiviral-innate-immunity-during-rna-virus-infection\/","title":{"rendered":"Genetic evidence for the suppressive role of zebrafish vhl targeting mavs in antiviral innate immunity during RNA virus infection"},"content":{"rendered":"<div>\n<p><b>J Immunol<\/b>. 2025 Jan 1;214(1):167-179. doi: 10.1093\/jimmun\/vkae017.<\/p>\n<p><b>ABSTRACT<\/b><\/p>\n<p>The von Hippel-Lindau (VHL) tumor suppressor gene VHL is a classic tumor suppressor that has been identified in family members with clear cell renal cell carcinomas, central nervous system and retinal hemangioblastomas, phaeochromocytomas, and pancreatic neuroendocrine tumors. The well-defined function of VHL is to mediate proteasomal degradation of hydroxylated hypoxia-inducible factor \u03b1 proteins, resulting in the downregulation of hypoxia-responsive gene expression. Previously, we reported that VHL inhibits antiviral signaling by targeting mitochondrial antiviral signaling protein (MAVS) for proteasomal degradation. However, due to the lack of a viable animal model, the physiological role and underlying mechanism of VHL in antiviral immunity remains to be elucidated. In this study, we found that heterozygous vhl-deficient zebrafish have normal neutrophils and no gross phenotypic alterations. However, upon spring viremia of carp virus or grass carp reovirus infection, antiviral gene expression is induced in vhl+\/- zebrafish compared with wild-type zebrafish. In addition, spring viremia of carp virus replication is suppressed in vhl+\/- zebrafish, owing to the enhancement of antiviral ability. Furthermore, by crossing with mavs-\/- zebrafish line, we observed that disruption of mavs in vhl+\/- zebrafish abrogates the viral resistance exhibited in vhl+\/- zebrafish. Thus, we reveal that heterozygous vhl deficiency enhances the antiviral ability of zebrafish against RNA virus infection, and we provide genetic evidence to support that zebrafish mavs serves as a mediator for the suppressive role of vhl in antiviral innate immunity.<\/p>\n<p>PMID:<a href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/40073266\/?utm_source=WordPress&amp;utm_medium=rss&amp;utm_content=2985117R&amp;ff=20250312185022&amp;v=2.18.0.post9+e462414\">40073266<\/a> | DOI:<a href=\"https:\/\/doi.org\/10.1093\/jimmun\/vkae017\">10.1093\/jimmun\/vkae017<\/a><\/p>\n<\/div>","protected":false},"excerpt":{"rendered":"<p>J Immunol. 2025 Jan 1;214(1):167-179. doi: 10.1093\/jimmun\/vkae017. ABSTRACT The von Hippel-Lindau (VHL) tumor suppressor gene VHL is a classic tumor suppressor that has been identified in family members with clear cell renal cell carcinomas, central nervous system and retinal hemangioblastomas, phaeochromocytomas, and pancreatic neuroendocrine tumors. The well-defined function of VHL is to mediate proteasomal degradation &#8230; <a title=\"Genetic evidence for the suppressive role of zebrafish vhl targeting mavs in antiviral innate immunity during RNA virus infection\" class=\"read-more\" href=\"https:\/\/inmuno.es\/index.php\/2025\/03\/12\/genetic-evidence-for-the-suppressive-role-of-zebrafish-vhl-targeting-mavs-in-antiviral-innate-immunity-during-rna-virus-infection\/\" aria-label=\"Read more about Genetic evidence for the suppressive role of zebrafish vhl targeting mavs in antiviral innate immunity during RNA virus infection\">Read more<\/a><\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[42,71],"tags":[],"class_list":["post-27118","post","type-post","status-publish","format-standard","hentry","category-publicaciones","category-the-journal-of-immunology"],"_links":{"self":[{"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/posts\/27118","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/comments?post=27118"}],"version-history":[{"count":0,"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/posts\/27118\/revisions"}],"wp:attachment":[{"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/media?parent=27118"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/categories?post=27118"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/tags?post=27118"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}