{"id":28533,"date":"2025-04-01T02:50:06","date_gmt":"2025-04-01T00:50:06","guid":{"rendered":"https:\/\/inmuno.es\/index.php\/2025\/04\/01\/single-cell-sequencing-reveals-the-same-heterogeneity-of-neutrophils-in-heatstroke-induced-lung-and-liver-injury-fuquan-wang\/"},"modified":"2025-04-01T02:50:06","modified_gmt":"2025-04-01T00:50:06","slug":"single-cell-sequencing-reveals-the-same-heterogeneity-of-neutrophils-in-heatstroke-induced-lung-and-liver-injury-fuquan-wang","status":"publish","type":"post","link":"https:\/\/inmuno.es\/index.php\/2025\/04\/01\/single-cell-sequencing-reveals-the-same-heterogeneity-of-neutrophils-in-heatstroke-induced-lung-and-liver-injury-fuquan-wang\/","title":{"rendered":"Single-cell sequencing reveals the same heterogeneity of neutrophils in heatstroke-induced lung and liver injury. Fuquan Wang"},"content":{"rendered":"<div>\n<p><b>Mucosal Immunol<\/b>. 2025 Mar 28:S1933-0219(25)00031-5. doi: 10.1016\/j.mucimm.2025.03.005. Online ahead of print.<\/p>\n<p><b>ABSTRACT<\/b><\/p>\n<p>Heatstroke (HS) is typically considered a sepsis-like syndrome caused by hyperthermia, often accompanied by multiple organ dysfunctions (MODS). To explore the mechanisms of MODS, we established a mouse model of HS by exposing mice to a hyperthermic and high-humidity environment. Then, we utilized single-cell RNA sequencing (scRNA-seq) to depict the cellular landscape of HS mice lung tissue and liver tissue. We found that the enhancement of neutrophil infiltration mediated by the &#8220;Cxcr2-Cxcl2\u2033 receptor-ligand pair is a prominent feature of HS-induced lung injury. By effectively suppressing the recruitment of neutrophils in HS-induced lung injury, the application of Cxcr2 inhibitor held positive implications for improving HS-induced lung injury. In addition to the chemotactic effect of immune cells on neutrophils, we identified a subcluster of fibroblasts labeled as Col14a1+, which possessed notable chemotactic factor-secretion characteristics and likely exerted a role in the early stages of neutrophil infiltration. Furthermore, our study unveiled significant heterogeneity among neutrophils within the HS-induced lung injury. Particularly, Cd177 + neutrophils exhibited a dominant presence, characterized by heightened pro-inflammatory responses and oxidative stress. In heatstroke-induced liver injury, neutrophils exhibited similar heterogeneous characteristics. Cd177 + neutrophils exhibited an enhanced ability to produce neutrophil extracellular traps (NETs) while lowering the levels of NETs can significantly improve heatstroke-induced lung and liver injury. Additionally, our study identified Cebpe as a key transcriptional regulatory factor in Cd177 + neutrophil differentiation. Knockdown of the expression of Cebpe can suppress the Cd177 + neutrophil differentiation and decrease the expression levels of NETs. Our research indicated a common heterogeneity in neutrophils during MODS in HS. Cd177 + neutrophils contributed to organ damage in HS, and Cebpe may serve as a crucial intervention target in the treatment of HS.<\/p>\n<p>PMID:<a href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/40158777\/?utm_source=WordPress&amp;utm_medium=rss&amp;utm_content=101299742&amp;ff=20250331205006&amp;v=2.18.0.post9+e462414\">40158777<\/a> | DOI:<a href=\"https:\/\/doi.org\/10.1016\/j.mucimm.2025.03.005\">10.1016\/j.mucimm.2025.03.005<\/a><\/p>\n<\/div>","protected":false},"excerpt":{"rendered":"<p>Mucosal Immunol. 2025 Mar 28:S1933-0219(25)00031-5. doi: 10.1016\/j.mucimm.2025.03.005. Online ahead of print. ABSTRACT Heatstroke (HS) is typically considered a sepsis-like syndrome caused by hyperthermia, often accompanied by multiple organ dysfunctions (MODS). To explore the mechanisms of MODS, we established a mouse model of HS by exposing mice to a hyperthermic and high-humidity environment. Then, we utilized &#8230; <a title=\"Single-cell sequencing reveals the same heterogeneity of neutrophils in heatstroke-induced lung and liver injury. Fuquan Wang\" class=\"read-more\" href=\"https:\/\/inmuno.es\/index.php\/2025\/04\/01\/single-cell-sequencing-reveals-the-same-heterogeneity-of-neutrophils-in-heatstroke-induced-lung-and-liver-injury-fuquan-wang\/\" aria-label=\"Read more about Single-cell sequencing reveals the same heterogeneity of neutrophils in heatstroke-induced lung and liver injury. Fuquan Wang\">Read more<\/a><\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[57,42],"tags":[],"class_list":["post-28533","post","type-post","status-publish","format-standard","hentry","category-mucosal-immunology","category-publicaciones"],"_links":{"self":[{"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/posts\/28533","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/comments?post=28533"}],"version-history":[{"count":0,"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/posts\/28533\/revisions"}],"wp:attachment":[{"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/media?parent=28533"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/categories?post=28533"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/tags?post=28533"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}