{"id":28595,"date":"2025-04-01T17:50:33","date_gmt":"2025-04-01T15:50:33","guid":{"rendered":"https:\/\/inmuno.es\/index.php\/2025\/04\/01\/accurate-enumeration-of-pathogen-specific-and-virtual-memory-cd8-t-cells-after-infection\/"},"modified":"2025-04-01T17:50:33","modified_gmt":"2025-04-01T15:50:33","slug":"accurate-enumeration-of-pathogen-specific-and-virtual-memory-cd8-t-cells-after-infection","status":"publish","type":"post","link":"https:\/\/inmuno.es\/index.php\/2025\/04\/01\/accurate-enumeration-of-pathogen-specific-and-virtual-memory-cd8-t-cells-after-infection\/","title":{"rendered":"Accurate enumeration of pathogen-specific and virtual memory CD8 T cells after infection"},"content":{"rendered":"
\n

J Immunol<\/b>. 2025 Apr 1:vkaf007. doi: 10.1093\/jimmun\/vkaf007. Online ahead of print.<\/p>\n

ABSTRACT<\/b><\/p>\n

Establishing the magnitude and kinetics of polyclonal Ag-specific CD8 T-cell responses, in addition to their functional fitness, is critical for evaluating a host’s ability to respond to different kinds of infections and\/or immunizations. To track CD8 T-cell responses during infection, a surrogate-activation-marker approach (CD8\u03b1loCD11ahi) is used to distinguish na\u00efve and Ag-experienced effector\/memory CD8 T cells in vivo. However, semidifferentiated virtual memory (Tvm) CD8 T cells have recently been identified in uninfected\/unmanipulated mice that display a phenotype similar to Ag-experienced cells. Therefore, magnitude and breadth of CD8 T-cell responses may be overestimated when responses are profiled using only CD8\u03b1\/CD11a markers. Thus, to precisely define and distinguish Tvm from pathogen-specific CD8 T cells during bacterial, parasitic, and viral infections, pathogen-specific sensor TCR-Tg cells were adoptively transferred prior to challenge. We demonstrate that Tvm CD8 T cells are found in CD8\u03b1loCD11ahi-defined Ag-experienced CD8 T cells but can be parsed out in infected host with their CD49d-CD44hiCD122hi expression pattern. However, this approach presents potential limitations as CD49d+ Ag-specific CD8 T cells can lose CD49d expression and adopt a Tvm-like phenotype depending on their Ag-stimulation history, age, and na\u00efve CD8 T-cell precursor frequency before the infection. Importantly, Tvm cells contribute to the breadth of the CD8 T-cell response, and their contribution depends on type of infection, time after infection, and tissue examined. Thus, these data define limitations in our ability to resolve between pathogen\/Ag-specific and Tvm CD8 T-cell responses during infection, a notion of direct relevance for experimental murine studies designed to follow CD8 T-cell responses in vivo.<\/p>\n

PMID:40167212<\/a> | DOI:10.1093\/jimmun\/vkaf007<\/a><\/p>\n<\/div>","protected":false},"excerpt":{"rendered":"

J Immunol. 2025 Apr 1:vkaf007. doi: 10.1093\/jimmun\/vkaf007. Online ahead of print. ABSTRACT Establishing the magnitude and kinetics of polyclonal Ag-specific CD8 T-cell responses, in addition to their functional fitness, is critical for evaluating a host’s ability to respond to different kinds of infections and\/or immunizations. To track CD8 T-cell responses during infection, a surrogate-activation-marker approach … Read more<\/a><\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[42,71],"tags":[],"class_list":["post-28595","post","type-post","status-publish","format-standard","hentry","category-publicaciones","category-the-journal-of-immunology"],"_links":{"self":[{"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/posts\/28595","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/comments?post=28595"}],"version-history":[{"count":0,"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/posts\/28595\/revisions"}],"wp:attachment":[{"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/media?parent=28595"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/categories?post=28595"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/tags?post=28595"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}