{"id":30275,"date":"2025-04-17T00:47:30","date_gmt":"2025-04-16T22:47:30","guid":{"rendered":"https:\/\/inmuno.es\/index.php\/2025\/04\/17\/efficient-generation-of-human-dendritic-cells-from-ipsc-by-introducing-a-feeder-free-expansion-step-for-hematopoietic-progenitorszahra-elahi-on-16-de-april-de-2025-at-1000\/"},"modified":"2025-04-17T00:47:30","modified_gmt":"2025-04-16T22:47:30","slug":"efficient-generation-of-human-dendritic-cells-from-ipsc-by-introducing-a-feeder-free-expansion-step-for-hematopoietic-progenitorszahra-elahi-on-16-de-april-de-2025-at-1000","status":"publish","type":"post","link":"https:\/\/inmuno.es\/index.php\/2025\/04\/17\/efficient-generation-of-human-dendritic-cells-from-ipsc-by-introducing-a-feeder-free-expansion-step-for-hematopoietic-progenitorszahra-elahi-on-16-de-april-de-2025-at-1000\/","title":{"rendered":"Efficient generation of human dendritic cells from iPSC by introducing a feeder-free expansion step for hematopoietic progenitors\u200bZahra Elahi   on 16 de April de 2025 at 10:00"},"content":{"rendered":"<div>\n<p><b>J Leukoc Biol<\/b>. 2025 Apr 16:qiaf045. doi: 10.1093\/jleuko\/qiaf045. Online ahead of print.<\/p>\n<p><b>ABSTRACT<\/b><\/p>\n<p>Dendritic cells (DCs) are rare innate immune cells that are essential regulators of anti-tumour, anti-viral and vaccine responses by the adaptive immune system. Conventional dendritic cells, particularly the cDC1 subset, are most desired for DC-based immunotherapies, however, it can be difficult to isolate sufficient numbers of primary cells from patients. The most common alternate sources of DC are ex vivo monocyte-derived DC, although patient-derived monocytes are often dysfunctional. Induced pluripotent stem cells (iPSC) offer a promising solution, providing an opportunity for in vitro generating DCs that are suitable for allogenic off-the-shelf batch-manufactured cells. Here, we developed an in vitro protocol designed to maximise the yield of iPSC-derived DC progenitors, with the specific goal of generating cDC1-like cells. The iPSC-DCs subsets generated by our method could be partitioned by cell surface phenotypes of cDC1, cDC2 and DC3, but they were most transcriptionally similar to monocyte-derived DC (MoDC). Stimulated iPSC-DCs generated pro-inflammatory cytokines, expressed migratory chemokine receptors including CCR7, upregulated co-stimulatory molecules and induced the proliferation of CD4\/CD8 T-cells. Altogether these data indicate that iPSC-derived DC have the potential to traffic through lymphatic endothelium and engage productively with T-cells. This method offers a promising step towards an expandable source of allogeneic human dendritic cells for future applications.<\/p>\n<p>PMID:<a href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/40238941\/?utm_source=WordPress&amp;utm_medium=rss&amp;utm_content=8405628&amp;ff=20250416184729&amp;v=2.18.0.post9+e462414\">40238941<\/a> | DOI:<a href=\"https:\/\/doi.org\/10.1093\/jleuko\/qiaf045\">10.1093\/jleuko\/qiaf045<\/a><\/p>\n<\/div>","protected":false},"excerpt":{"rendered":"<p>J Leukoc Biol. 2025 Apr 16:qiaf045. doi: 10.1093\/jleuko\/qiaf045. Online ahead of print. ABSTRACT Dendritic cells (DCs) are rare innate immune cells that are essential regulators of anti-tumour, anti-viral and vaccine responses by the adaptive immune system. Conventional dendritic cells, particularly the cDC1 subset, are most desired for DC-based immunotherapies, however, it can be difficult to &#8230; <a title=\"Efficient generation of human dendritic cells from iPSC by introducing a feeder-free expansion step for hematopoietic progenitors\u200bZahra Elahi   on 16 de April de 2025 at 10:00\" class=\"read-more\" href=\"https:\/\/inmuno.es\/index.php\/2025\/04\/17\/efficient-generation-of-human-dendritic-cells-from-ipsc-by-introducing-a-feeder-free-expansion-step-for-hematopoietic-progenitorszahra-elahi-on-16-de-april-de-2025-at-1000\/\" aria-label=\"Read more about Efficient generation of human dendritic cells from iPSC by introducing a feeder-free expansion step for hematopoietic progenitors\u200bZahra Elahi   on 16 de April de 2025 at 10:00\">Read more<\/a><\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[86,42],"tags":[],"class_list":["post-30275","post","type-post","status-publish","format-standard","hentry","category-journal-of-leukocyte-biology","category-publicaciones"],"_links":{"self":[{"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/posts\/30275","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/comments?post=30275"}],"version-history":[{"count":0,"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/posts\/30275\/revisions"}],"wp:attachment":[{"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/media?parent=30275"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/categories?post=30275"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/tags?post=30275"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}