{"id":30842,"date":"2025-04-26T22:47:26","date_gmt":"2025-04-26T20:47:26","guid":{"rendered":"https:\/\/inmuno.es\/index.php\/2025\/04\/26\/b-cell-expressed-cd1d-promotes-mpl-tdcm-lipid-emulsion-adjuvant-effects-in-polysaccharide-vaccines\/"},"modified":"2025-04-26T22:47:26","modified_gmt":"2025-04-26T20:47:26","slug":"b-cell-expressed-cd1d-promotes-mpl-tdcm-lipid-emulsion-adjuvant-effects-in-polysaccharide-vaccines","status":"publish","type":"post","link":"https:\/\/inmuno.es\/index.php\/2025\/04\/26\/b-cell-expressed-cd1d-promotes-mpl-tdcm-lipid-emulsion-adjuvant-effects-in-polysaccharide-vaccines\/","title":{"rendered":"B cell-expressed CD1d promotes MPL\/TDCM lipid emulsion adjuvant effects in polysaccharide vaccines"},"content":{"rendered":"<div>\n<p><b>J Immunol<\/b>. 2025 Apr 25:vkaf074. doi: 10.1093\/jimmun\/vkaf074. Online ahead of print.<\/p>\n<p><b>ABSTRACT<\/b><\/p>\n<p>T cell-independent type 2 antigens (TI-2 Ags), such as pneumococcal polysaccharides, elicit weak immunoglobulin G (IgG) responses and are refractive to boosting. Overcoming this challenge is critical for improving vaccines. Previously, we demonstrated a lipid-based adjuvant composed of monophosphoryl lipid A, synthetic cord factor, and squalene significantly boosts primary and secondary IgM and IgG production against polysaccharide Ags. Herein, we show beta-2 microglobulin, but not MHC class II, is essential for adjuvant-induced increases in polysaccharide-specific IgG levels. Furthermore, we demonstrate CD1d expression is essential for optimal adjuvant-induced increases in IgG, but is not required for IgG responses to TI-2 Ags administered without adjuvant, with the exception of the bacterial cell wall polysaccharide component, phosphorylcholine. Adoptive transfer of splenic and peritoneal cells from VHB1-8 transgenic mice into CD1d-\/- mice revealed adjuvant-induced increases in NP-Ficoll-specific IgG production by CD1d+\/+ transgenic B cells, but not recipient B cells, suggesting B cell-expressed CD1d is critical for adjuvant-induced effects on TI-2 antibody responses. Consistent with this, bone marrow chimera mice with selective CD1d deficiency in B cells demonstrated B cell-expressed CD1d was dispensable for iNKT cell development and maintenance but was required for adjuvant-induced increases in protective levels of polysaccharide- and phosphorylcholine-specific IgG. Notably, both iNKT cells and CD1d crosslinking significantly increased IgG production by B cells coactivated with TI-2 Ag and adjuvant, suggesting iNKT-induced CD1d signaling may promote increased IgG production. In summary, our study reveals B cell-dependent CD1d expression is critical for effectiveness of a potent lipid-based adjuvant in augmenting polysaccharide- and phosphorylcholine-specific IgG responses.<\/p>\n<p>PMID:<a href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/40280183\/?utm_source=WordPress&amp;utm_medium=rss&amp;utm_content=2985117R&amp;ff=20250426164725&amp;v=2.18.0.post9+e462414\">40280183<\/a> | DOI:<a href=\"https:\/\/doi.org\/10.1093\/jimmun\/vkaf074\">10.1093\/jimmun\/vkaf074<\/a><\/p>\n<\/div>","protected":false},"excerpt":{"rendered":"<p>J Immunol. 2025 Apr 25:vkaf074. doi: 10.1093\/jimmun\/vkaf074. Online ahead of print. ABSTRACT T cell-independent type 2 antigens (TI-2 Ags), such as pneumococcal polysaccharides, elicit weak immunoglobulin G (IgG) responses and are refractive to boosting. Overcoming this challenge is critical for improving vaccines. Previously, we demonstrated a lipid-based adjuvant composed of monophosphoryl lipid A, synthetic cord &#8230; <a title=\"B cell-expressed CD1d promotes MPL\/TDCM lipid emulsion adjuvant effects in polysaccharide vaccines\" class=\"read-more\" href=\"https:\/\/inmuno.es\/index.php\/2025\/04\/26\/b-cell-expressed-cd1d-promotes-mpl-tdcm-lipid-emulsion-adjuvant-effects-in-polysaccharide-vaccines\/\" aria-label=\"Read more about B cell-expressed CD1d promotes MPL\/TDCM lipid emulsion adjuvant effects in polysaccharide vaccines\">Read more<\/a><\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[42,71],"tags":[],"class_list":["post-30842","post","type-post","status-publish","format-standard","hentry","category-publicaciones","category-the-journal-of-immunology"],"_links":{"self":[{"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/posts\/30842","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/comments?post=30842"}],"version-history":[{"count":0,"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/posts\/30842\/revisions"}],"wp:attachment":[{"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/media?parent=30842"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/categories?post=30842"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/tags?post=30842"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}