{"id":33360,"date":"2025-05-25T06:46:59","date_gmt":"2025-05-25T04:46:59","guid":{"rendered":"https:\/\/inmuno.es\/index.php\/2025\/05\/25\/macrophage-polarization-inflammatory-monocytes-and-impaired-mdscs-are-associated-with-murine-and-human-immune-aplastic-anemiajoshua-glass-on-24-de-may-de-2025-at-1000\/"},"modified":"2025-05-25T06:46:59","modified_gmt":"2025-05-25T04:46:59","slug":"macrophage-polarization-inflammatory-monocytes-and-impaired-mdscs-are-associated-with-murine-and-human-immune-aplastic-anemiajoshua-glass-on-24-de-may-de-2025-at-1000","status":"publish","type":"post","link":"https:\/\/inmuno.es\/index.php\/2025\/05\/25\/macrophage-polarization-inflammatory-monocytes-and-impaired-mdscs-are-associated-with-murine-and-human-immune-aplastic-anemiajoshua-glass-on-24-de-may-de-2025-at-1000\/","title":{"rendered":"Macrophage Polarization, Inflammatory Monocytes, and Impaired MDSCs are Associated with Murine and Human Immune Aplastic Anemia\u200bJoshua Glass   on 24 de May de 2025 at 10:00"},"content":{"rendered":"<div>\n<p><b>J Leukoc Biol<\/b>. 2025 May 24:qiaf073. doi: 10.1093\/jleuko\/qiaf073. Online ahead of print.<\/p>\n<p><b>ABSTRACT<\/b><\/p>\n<p>Immune-mediated bone marrow failure (BMF) entails a complex immune landscape. Myeloid cells, including monocytes, macrophages, and myeloid-derived suppressor cells (MDSCs), are involved in the development and progression of immune aplastic anemia (AA). We used a murine model of BMF to explore the effects of CSF-1R inhibition on immune pathophysiology. Hematopoiesis, immune cell populations, and gene expression were assessed by flow cytometry, cytokine analysis, and single-cell RNA sequencing. CSF-1R inhibition with the small molecule PLX3397 intensified BMF in CByB6F1 mice, enhancing inflammation and macrophage polarization toward the pro-inflammatory M1 phenotype. This was accompanied by increased leukocyte apoptosis, a reduction in CD11b+ myeloid cells, and worsened animal survival. In contrast, the JAK-inhibitor baricitinib attenuated BMF, promoting M2 macrophage polarization, and decreasing CD8+ T cell infiltration of bone marrow. Single-cell RNA analysis revealed upregulation of M1 signature genes in both murine BMF and also AA human samples. In patients with severe AA, there was a shift towards an M1-like monocyte phenotype, correlating with increased inflammatory cytokine expression and altered MDSC populations. These findings highlight the role of myeloid-derived cells in BMF and suggest that M1 macrophages, with defective MDSC function, contribute to disease pathogenesis and progression. Targeting macrophage polarization or MDSCs offers alternative therapeutic strategies in immune-mediated BMF.<\/p>\n<p>PMID:<a href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/40411822\/?utm_source=WordPress&amp;utm_medium=rss&amp;utm_content=8405628&amp;ff=20250525004652&amp;v=2.18.0.post9+e462414\">40411822<\/a> | DOI:<a href=\"https:\/\/doi.org\/10.1093\/jleuko\/qiaf073\">10.1093\/jleuko\/qiaf073<\/a><\/p>\n<\/div>","protected":false},"excerpt":{"rendered":"<p>J Leukoc Biol. 2025 May 24:qiaf073. doi: 10.1093\/jleuko\/qiaf073. Online ahead of print. ABSTRACT Immune-mediated bone marrow failure (BMF) entails a complex immune landscape. Myeloid cells, including monocytes, macrophages, and myeloid-derived suppressor cells (MDSCs), are involved in the development and progression of immune aplastic anemia (AA). We used a murine model of BMF to explore the &#8230; <a title=\"Macrophage Polarization, Inflammatory Monocytes, and Impaired MDSCs are Associated with Murine and Human Immune Aplastic Anemia\u200bJoshua Glass   on 24 de May de 2025 at 10:00\" class=\"read-more\" href=\"https:\/\/inmuno.es\/index.php\/2025\/05\/25\/macrophage-polarization-inflammatory-monocytes-and-impaired-mdscs-are-associated-with-murine-and-human-immune-aplastic-anemiajoshua-glass-on-24-de-may-de-2025-at-1000\/\" aria-label=\"Read more about Macrophage Polarization, Inflammatory Monocytes, and Impaired MDSCs are Associated with Murine and Human Immune Aplastic Anemia\u200bJoshua Glass   on 24 de May de 2025 at 10:00\">Read more<\/a><\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[86,42],"tags":[],"class_list":["post-33360","post","type-post","status-publish","format-standard","hentry","category-journal-of-leukocyte-biology","category-publicaciones"],"_links":{"self":[{"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/posts\/33360","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/comments?post=33360"}],"version-history":[{"count":0,"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/posts\/33360\/revisions"}],"wp:attachment":[{"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/media?parent=33360"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/categories?post=33360"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/tags?post=33360"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}