{"id":37010,"date":"2025-07-07T12:54:14","date_gmt":"2025-07-07T10:54:14","guid":{"rendered":"https:\/\/inmuno.es\/index.php\/2025\/07\/07\/mir-124-3p-derived-from-plasma-exosomes-enhances-m2-macrophage-polarization-to-treat-acute-lung-injury\/"},"modified":"2025-07-07T12:54:14","modified_gmt":"2025-07-07T10:54:14","slug":"mir-124-3p-derived-from-plasma-exosomes-enhances-m2-macrophage-polarization-to-treat-acute-lung-injury","status":"publish","type":"post","link":"https:\/\/inmuno.es\/index.php\/2025\/07\/07\/mir-124-3p-derived-from-plasma-exosomes-enhances-m2-macrophage-polarization-to-treat-acute-lung-injury\/","title":{"rendered":"miR-124-3p derived from plasma exosomes enhances M2 macrophage polarization to treat acute lung injury"},"content":{"rendered":"<div>\n<p><b>J Immunol<\/b>. 2025 Jul 7:vkaf097. doi: 10.1093\/jimmun\/vkaf097. Online ahead of print.<\/p>\n<p><b>ABSTRACT<\/b><\/p>\n<p>Acute lung injury (ALI) post-lung transplantation (LT) is a major clinical challenge. This study investigates the role of exosomal miR-124-3p in modulating macrophage polarization and ameliorating ALI. Using male C57BL\/6J mice, we established a left lung orthotopic transplantation model. Transcriptomic analysis revealed that miR-124-3p was significantly downregulated in the plasma exosomes of LT mice. Functional experiments demonstrated that plasma exosomal miR-124-3p promotes M2 macrophage polarization by targeting Kr\u00fcppel-like factor 6 (KLF6) and inhibiting the NF-\u03baB pathway. Overexpression of miR-124-3p significantly reduced inflammation, enhanced lung tissue repair, and improved oxygenation indices in vivo. In vitro, exosomal miR-124-3p reduced M1 markers (iNOS, IL-1\u03b2, IL-6) and increased M2 markers (Arg1, Ym1, Fizz1) in macrophages, confirmed by flow cytometry and Western blot. Furthermore, overexpression of KLF6 reversed the therapeutic effects of miR-124-3p. This study identifies miR-124-3p as a critical regulator of macrophage polarization and ALI pathophysiology, providing a potential therapeutic target for managing ALI in lung transplant recipients.<\/p>\n<p>PMID:<a href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/40619875\/?utm_source=SimplePie&amp;utm_medium=rss&amp;utm_content=2985117R&amp;ff=20250707065412&amp;v=2.18.0.post9+e462414\">40619875<\/a> | DOI:<a href=\"https:\/\/doi.org\/10.1093\/jimmun\/vkaf097\">10.1093\/jimmun\/vkaf097<\/a><\/p>\n<\/div>","protected":false},"excerpt":{"rendered":"<p>J Immunol. 2025 Jul 7:vkaf097. doi: 10.1093\/jimmun\/vkaf097. Online ahead of print. ABSTRACT Acute lung injury (ALI) post-lung transplantation (LT) is a major clinical challenge. This study investigates the role of exosomal miR-124-3p in modulating macrophage polarization and ameliorating ALI. Using male C57BL\/6J mice, we established a left lung orthotopic transplantation model. Transcriptomic analysis revealed that &#8230; <a title=\"miR-124-3p derived from plasma exosomes enhances M2 macrophage polarization to treat acute lung injury\" class=\"read-more\" href=\"https:\/\/inmuno.es\/index.php\/2025\/07\/07\/mir-124-3p-derived-from-plasma-exosomes-enhances-m2-macrophage-polarization-to-treat-acute-lung-injury\/\" aria-label=\"Read more about miR-124-3p derived from plasma exosomes enhances M2 macrophage polarization to treat acute lung injury\">Read more<\/a><\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[42,71],"tags":[],"class_list":["post-37010","post","type-post","status-publish","format-standard","hentry","category-publicaciones","category-the-journal-of-immunology"],"_links":{"self":[{"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/posts\/37010","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/comments?post=37010"}],"version-history":[{"count":0,"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/posts\/37010\/revisions"}],"wp:attachment":[{"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/media?parent=37010"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/categories?post=37010"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/tags?post=37010"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}