{"id":37564,"date":"2025-07-09T12:47:07","date_gmt":"2025-07-09T10:47:07","guid":{"rendered":"https:\/\/inmuno.es\/index.php\/2025\/07\/09\/myeloid-specific-deficiency-of-ribosomal-protein-l13a-alters-macrophage-polarity-and-diversity-during-differentiation-from-bone-marrowantara-roy-on-9-de-july-de-2025-at-1000\/"},"modified":"2025-07-09T12:47:07","modified_gmt":"2025-07-09T10:47:07","slug":"myeloid-specific-deficiency-of-ribosomal-protein-l13a-alters-macrophage-polarity-and-diversity-during-differentiation-from-bone-marrowantara-roy-on-9-de-july-de-2025-at-1000","status":"publish","type":"post","link":"https:\/\/inmuno.es\/index.php\/2025\/07\/09\/myeloid-specific-deficiency-of-ribosomal-protein-l13a-alters-macrophage-polarity-and-diversity-during-differentiation-from-bone-marrowantara-roy-on-9-de-july-de-2025-at-1000\/","title":{"rendered":"Myeloid-specific deficiency of ribosomal protein L13a alters macrophage polarity and diversity during differentiation from bone marrow\u200bAntara Roy   on 9 de July de 2025 at 10:00"},"content":{"rendered":"<div>\n<p><b>J Leukoc Biol<\/b>. 2025 Jul 9:qiaf102. doi: 10.1093\/jleuko\/qiaf102. Online ahead of print.<\/p>\n<p><b>ABSTRACT<\/b><\/p>\n<p>Macrophages show substantial plasticity, leading to a diverse population of these cells with different states of polarization during differentiation from bone marrow. However, the mechanisms underlying this process are not well understood. Here, we identified a novel role of ribosomal protein L13a previously shown to be engaged in the physiological control of inflammation regulating macrophage diversity and polarity. Using an ex-vivo differentiation model of bone marrow-derived macrophages (BMDM) from the control (L13aflox\/flox) and myeloid-specific L13a KO (L13aflox\/flox LysMCre+) mice (L13a-KO) we present compelling evidence of the role of L13a in regulating macrophage polarization that goes beyond the M1-M2-based binary concept. We show that macrophages from L13a-KO mice lead to enhanced expression of classical markers of both M1 and M2 and surprising deviation from the expected response under known inducers of polarity. The phosphorylation-dependent activation of a number of signaling molecules played a role in this process. Bulk RNA and single-cell RNA sequencing of the BMDM from the L13a-KO mice show widespread change in overall gene expression and robust differences in the diverse populations of the bone marrow-derived cells from the control and KO mice. In addition, this study also shows a substantial increase of Th1 and Th2 signature genes in CD4+ T cells isolated from the L13a-KO animals. Together, our studies provide new insights into the regulations of macrophage polarization by L13a-driven novel intermediate effectors or mediators.<\/p>\n<p>PMID:<a href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/40631353\/?utm_source=SimplePie&amp;utm_medium=rss&amp;utm_content=8405628&amp;ff=20250709064706&amp;v=2.18.0.post9+e462414\">40631353<\/a> | DOI:<a href=\"https:\/\/doi.org\/10.1093\/jleuko\/qiaf102\">10.1093\/jleuko\/qiaf102<\/a><\/p>\n<\/div>","protected":false},"excerpt":{"rendered":"<p>J Leukoc Biol. 2025 Jul 9:qiaf102. doi: 10.1093\/jleuko\/qiaf102. Online ahead of print. ABSTRACT Macrophages show substantial plasticity, leading to a diverse population of these cells with different states of polarization during differentiation from bone marrow. However, the mechanisms underlying this process are not well understood. Here, we identified a novel role of ribosomal protein L13a &#8230; <a title=\"Myeloid-specific deficiency of ribosomal protein L13a alters macrophage polarity and diversity during differentiation from bone marrow\u200bAntara Roy   on 9 de July de 2025 at 10:00\" class=\"read-more\" href=\"https:\/\/inmuno.es\/index.php\/2025\/07\/09\/myeloid-specific-deficiency-of-ribosomal-protein-l13a-alters-macrophage-polarity-and-diversity-during-differentiation-from-bone-marrowantara-roy-on-9-de-july-de-2025-at-1000\/\" aria-label=\"Read more about Myeloid-specific deficiency of ribosomal protein L13a alters macrophage polarity and diversity during differentiation from bone marrow\u200bAntara Roy   on 9 de July de 2025 at 10:00\">Read more<\/a><\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[86,42],"tags":[],"class_list":["post-37564","post","type-post","status-publish","format-standard","hentry","category-journal-of-leukocyte-biology","category-publicaciones"],"_links":{"self":[{"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/posts\/37564","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/comments?post=37564"}],"version-history":[{"count":0,"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/posts\/37564\/revisions"}],"wp:attachment":[{"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/media?parent=37564"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/categories?post=37564"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/tags?post=37564"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}