{"id":37907,"date":"2025-07-10T12:00:00","date_gmt":"2025-07-10T10:00:00","guid":{"rendered":"https:\/\/inmuno.es\/index.php\/2025\/07\/10\/evaluation-of-a-multiplex-electrochemiluminescence-assay-for-detection-of-anti-pneumococcal-antibodies-in-the-diagnosis-of-selective-polysaccharide-antibody-deficiency\/"},"modified":"2025-07-10T12:00:00","modified_gmt":"2025-07-10T10:00:00","slug":"evaluation-of-a-multiplex-electrochemiluminescence-assay-for-detection-of-anti-pneumococcal-antibodies-in-the-diagnosis-of-selective-polysaccharide-antibody-deficiency","status":"publish","type":"post","link":"https:\/\/inmuno.es\/index.php\/2025\/07\/10\/evaluation-of-a-multiplex-electrochemiluminescence-assay-for-detection-of-anti-pneumococcal-antibodies-in-the-diagnosis-of-selective-polysaccharide-antibody-deficiency\/","title":{"rendered":"Evaluation of a Multiplex Electrochemiluminescence Assay for Detection of Anti-Pneumococcal Antibodies in the Diagnosis of Selective Polysaccharide Antibody Deficiency"},"content":{"rendered":"<div>\n<p><b>J Clin Immunol<\/b>. 2025 Jul 10;45(1):112. doi: 10.1007\/s10875-025-01911-0.<\/p>\n<p><b>ABSTRACT<\/b><\/p>\n<p>Streptococcus pneumoniae can be responsible for severe infections, especially in patients with primary antibody deficiencies like selective anti-polysaccharide antibodies deficiency (SPAD). The reference method recommaned by the World Health Organization for assessment of anti-pneumococcal capsular polysaccharides (PCPs) IgG antibodies is a standardized serotype-specific ELISA (WHO-SSA), but this manual method is time-consuming and limit the number of evaluated PCPs. We aim to evaluate the performance values of a multiplex assay based on electrochemiluminescence (ECL-plex). A panel of 164 sera from 82 patients sampled before and 4-8 weeks after immunization by the 23-valent pneumococcal polysaccharide vaccine (PPV23) were assessed by the reference WHO-SSA (7 to 13 serotypes) and by an 18-plex ECL assay (18 serotypes). All patients had normal serum Ig\/subclasses levels and were classified as good (n = 43) or poor responders (n = 39, i.e. SPAD patients) according to the American Academy of Asthma, Allergy and Immunology&#8217;s (AAAAI) current guidelines. We observed excellent correlations between the two methods for anti-PCPs titers against 7 serotypes (r = 0.88 [95% CI: 0.87-0.90], n = 124 sera) and 13 serotypes (r = 0.87 [0.87-0.89], n = 40 sera). Using the AAAAI&#8217;s guidelines for interpretation, the test performance of the 18-plex ECL assay for SPAD diagnosis showed a sensitivity of 95% and specificity of 84%, positive and negative predictive values of 84% and 95%, respectively. The percentage of agreement was 89% between the SSA and the 18-plex ECL assay. The 18-plex ECL assay is a reliable, rapid, and simple method for evaluating anti-PCPs response and screening for SPAD diagnosis.<\/p>\n<p>PMID:<a href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/40637813\/?utm_source=SimplePie&amp;utm_medium=rss&amp;utm_campaign=journals&amp;utm_content=8102137&amp;ff=20250710124750&amp;v=2.18.0.post9+e462414\">40637813<\/a> | DOI:<a href=\"https:\/\/doi.org\/10.1007\/s10875-025-01911-0\">10.1007\/s10875-025-01911-0<\/a><\/p>\n<\/div>","protected":false},"excerpt":{"rendered":"<p>J Clin Immunol. 2025 Jul 10;45(1):112. doi: 10.1007\/s10875-025-01911-0. ABSTRACT Streptococcus pneumoniae can be responsible for severe infections, especially in patients with primary antibody deficiencies like selective anti-polysaccharide antibodies deficiency (SPAD). The reference method recommaned by the World Health Organization for assessment of anti-pneumococcal capsular polysaccharides (PCPs) IgG antibodies is a standardized serotype-specific ELISA (WHO-SSA), but &#8230; <a title=\"Evaluation of a Multiplex Electrochemiluminescence Assay for Detection of Anti-Pneumococcal Antibodies in the Diagnosis of Selective Polysaccharide Antibody Deficiency\" class=\"read-more\" href=\"https:\/\/inmuno.es\/index.php\/2025\/07\/10\/evaluation-of-a-multiplex-electrochemiluminescence-assay-for-detection-of-anti-pneumococcal-antibodies-in-the-diagnosis-of-selective-polysaccharide-antibody-deficiency\/\" aria-label=\"Read more about Evaluation of a Multiplex Electrochemiluminescence Assay for Detection of Anti-Pneumococcal Antibodies in the Diagnosis of Selective Polysaccharide Antibody Deficiency\">Read more<\/a><\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[69,42],"tags":[],"class_list":["post-37907","post","type-post","status-publish","format-standard","hentry","category-journal-of-clinical-immunology","category-publicaciones"],"_links":{"self":[{"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/posts\/37907","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/comments?post=37907"}],"version-history":[{"count":0,"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/posts\/37907\/revisions"}],"wp:attachment":[{"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/media?parent=37907"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/categories?post=37907"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/tags?post=37907"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}