{"id":38624,"date":"2025-07-22T00:47:07","date_gmt":"2025-07-21T22:47:07","guid":{"rendered":"https:\/\/inmuno.es\/index.php\/2025\/07\/22\/il-23-promotes-t-cell-trafficking-in-experimental-autoimmune-myocarditis\/"},"modified":"2025-07-22T00:47:07","modified_gmt":"2025-07-21T22:47:07","slug":"il-23-promotes-t-cell-trafficking-in-experimental-autoimmune-myocarditis","status":"publish","type":"post","link":"https:\/\/inmuno.es\/index.php\/2025\/07\/22\/il-23-promotes-t-cell-trafficking-in-experimental-autoimmune-myocarditis\/","title":{"rendered":"IL-23 promotes T cell trafficking in experimental autoimmune myocarditis"},"content":{"rendered":"<div>\n<p><b>J Immunol<\/b>. 2025 Jul 21:vkaf150. doi: 10.1093\/jimmun\/vkaf150. Online ahead of print.<\/p>\n<p><b>ABSTRACT<\/b><\/p>\n<p>Th1 and Th17 cell-mediated autoimmunity is critical for myocarditis induction. Antigen-presenting cell (APCs)-released interleukin (IL)-12 and IL-23 are implicated in the differentiation of Th1 and Th17 lineages. In this study, we utilized cardiac self-antigen myosin heavy chain alpha (\u03b1-MyHC)-pulsed bone marrow-derived dendritic cells (bmDCs) and wild-type, IL-12p35-\/-, and IL-23p19-\/- mice to investigate the influence of IL-12 and IL-23 on CD4+ T cells in experimental autoimmune myocarditis (EAM). All mice (Mus musculus) receiving \u03b1-MyHC-pulsed bmDCs developed acute myocarditis and accumulated interferon (IFN)-\u03b3-positive and IL-17A-positive CD4+ T cells in cardiac tissue. Compared to immunization with wild-type bmDCs, adoptive transfer of \u03b1-MyHC-pulsed IL-23p19-\/- bmDCs resulted in decreased numbers of IL-17A+CD4+ T cells and in a twofold reduction of infiltrating T lymphocytes in the hearts of recipient mice, despite unaffected infiltration count of CD45+ leukocytes. In contrast, IL-12p35-\/- bmDCs induced fewer IFN-\u03b3-producing CD4+ T cells but did not affect T lymphocyte infiltration. Furthermore, IL-23p19-\/- recipient mice showed reduced heart-infiltrating CD3+ T cells, but not total CD45+, compared to wild-type mice after adoptive transfer of \u03b1-MyHC-pulsed IL-23p19-\/- bmDCs. Likewise, in the transgenic TCRM model of EAM, TCRMxIL-23p19-\/- mice showed reduced myocarditis severity and fewer T lymphocytes in their hearts pointing to impaired T cell trafficking in absence of IL-23. We validated the pro-migratory effect of IL-23 on activated CD4+ T cells in vitro and demonstrated an essential role of Rho GTPases in this process. Our findings provide new insights into the pro-inflammatory activity of IL-23 in autoimmune myocarditis, highlighting its unique role in promoting T cell migration.<\/p>\n<p>PMID:<a href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/40690778\/?utm_source=SimplePie&amp;utm_medium=rss&amp;utm_content=2985117R&amp;ff=20250721184704&amp;v=2.18.0.post9+e462414\">40690778<\/a> | DOI:<a href=\"https:\/\/doi.org\/10.1093\/jimmun\/vkaf150\">10.1093\/jimmun\/vkaf150<\/a><\/p>\n<\/div>","protected":false},"excerpt":{"rendered":"<p>J Immunol. 2025 Jul 21:vkaf150. doi: 10.1093\/jimmun\/vkaf150. Online ahead of print. ABSTRACT Th1 and Th17 cell-mediated autoimmunity is critical for myocarditis induction. Antigen-presenting cell (APCs)-released interleukin (IL)-12 and IL-23 are implicated in the differentiation of Th1 and Th17 lineages. In this study, we utilized cardiac self-antigen myosin heavy chain alpha (\u03b1-MyHC)-pulsed bone marrow-derived dendritic cells &#8230; <a title=\"IL-23 promotes T cell trafficking in experimental autoimmune myocarditis\" class=\"read-more\" href=\"https:\/\/inmuno.es\/index.php\/2025\/07\/22\/il-23-promotes-t-cell-trafficking-in-experimental-autoimmune-myocarditis\/\" aria-label=\"Read more about IL-23 promotes T cell trafficking in experimental autoimmune myocarditis\">Read more<\/a><\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[42,71],"tags":[],"class_list":["post-38624","post","type-post","status-publish","format-standard","hentry","category-publicaciones","category-the-journal-of-immunology"],"_links":{"self":[{"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/posts\/38624","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/comments?post=38624"}],"version-history":[{"count":0,"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/posts\/38624\/revisions"}],"wp:attachment":[{"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/media?parent=38624"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/categories?post=38624"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/tags?post=38624"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}