{"id":39173,"date":"2025-07-29T07:47:31","date_gmt":"2025-07-29T05:47:31","guid":{"rendered":"https:\/\/inmuno.es\/index.php\/2025\/07\/29\/single-cell-transcriptional-analysis-of-human-adenoids-identifies-molecular-features-of-airway-microfold-cells-samuel-alvarez-arguedas\/"},"modified":"2025-07-29T07:47:31","modified_gmt":"2025-07-29T05:47:31","slug":"single-cell-transcriptional-analysis-of-human-adenoids-identifies-molecular-features-of-airway-microfold-cells-samuel-alvarez-arguedas","status":"publish","type":"post","link":"https:\/\/inmuno.es\/index.php\/2025\/07\/29\/single-cell-transcriptional-analysis-of-human-adenoids-identifies-molecular-features-of-airway-microfold-cells-samuel-alvarez-arguedas\/","title":{"rendered":"Single cell transcriptional analysis of human adenoids identifies molecular features of airway microfold cells. Samuel Alvarez-Arguedas"},"content":{"rendered":"<div>\n<p><b>Mucosal Immunol<\/b>. 2025 Jul 26:S1933-0219(25)00077-7. doi: 10.1016\/j.mucimm.2025.07.006. Online ahead of print.<\/p>\n<p><b>ABSTRACT<\/b><\/p>\n<p>The nasal, oropharyngeal, and bronchial mucosa are primary contact points for airborne pathogens like Mycobacterium tuberculosis (Mtb), SARS-CoV-2, and influenza virus. While mucosal surfaces can function as both entry points and barriers to infection, mucosa-associated lymphoid tissues (MALT) facilitate early immune responses to mucosal antigens. MALT contains a variety of specialized epithelial cells, including a rare cell type called a microfold cell (M cell) that functions to transport apical antigens to basolateral antigen-presenting cells, a crucial step in the initiation of mucosal immunity. M cells have been extensively characterized in the gastrointestinal (GI) tract in murine and human models. However, the precise development and functions of human airway M cells are unknown. Here, using single-nucleus RNA sequencing (snRNA-seq), we generated an atlas of cells from the human adenoid and identified 26 unique cell types representing basal, club, hillock, and hematopoietic lineages, defined their developmental trajectories, and determined cell-cell relationships. Using trajectory analysis, we found that human airway M cells develop from progenitor club cells and express a gene signature distinct from intestinal M cells. Surprisingly, we also identified a heretofore unknown epithelial cell type demonstrating a robust interferon-stimulated gene signature. Our analysis of human adenoid cells enhances our understanding of mucosal immune responses and the role of M cells in airway immunity. This work also provides a resource for understanding early interactions of pathogens with airway mucosa and a platform for development of mucosal vaccines.<\/p>\n<p>PMID:<a href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/40721095\/?utm_source=SimplePie&amp;utm_medium=rss&amp;utm_content=101299742&amp;ff=20250729014728&amp;v=2.18.0.post9+e462414\">40721095<\/a> | DOI:<a href=\"https:\/\/doi.org\/10.1016\/j.mucimm.2025.07.006\">10.1016\/j.mucimm.2025.07.006<\/a><\/p>\n<\/div>","protected":false},"excerpt":{"rendered":"<p>Mucosal Immunol. 2025 Jul 26:S1933-0219(25)00077-7. doi: 10.1016\/j.mucimm.2025.07.006. Online ahead of print. ABSTRACT The nasal, oropharyngeal, and bronchial mucosa are primary contact points for airborne pathogens like Mycobacterium tuberculosis (Mtb), SARS-CoV-2, and influenza virus. While mucosal surfaces can function as both entry points and barriers to infection, mucosa-associated lymphoid tissues (MALT) facilitate early immune responses to &#8230; <a title=\"Single cell transcriptional analysis of human adenoids identifies molecular features of airway microfold cells. Samuel Alvarez-Arguedas\" class=\"read-more\" href=\"https:\/\/inmuno.es\/index.php\/2025\/07\/29\/single-cell-transcriptional-analysis-of-human-adenoids-identifies-molecular-features-of-airway-microfold-cells-samuel-alvarez-arguedas\/\" aria-label=\"Read more about Single cell transcriptional analysis of human adenoids identifies molecular features of airway microfold cells. Samuel Alvarez-Arguedas\">Read more<\/a><\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[57,42],"tags":[],"class_list":["post-39173","post","type-post","status-publish","format-standard","hentry","category-mucosal-immunology","category-publicaciones"],"_links":{"self":[{"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/posts\/39173","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/comments?post=39173"}],"version-history":[{"count":0,"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/posts\/39173\/revisions"}],"wp:attachment":[{"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/media?parent=39173"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/categories?post=39173"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/tags?post=39173"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}