{"id":39538,"date":"2025-08-02T16:47:23","date_gmt":"2025-08-02T14:47:23","guid":{"rendered":"https:\/\/inmuno.es\/index.php\/2025\/08\/02\/genomic-specificity-of-anti-mouse-tcr-mabs-determined-by-single-cell-rnaseq\/"},"modified":"2025-08-02T16:47:23","modified_gmt":"2025-08-02T14:47:23","slug":"genomic-specificity-of-anti-mouse-tcr-mabs-determined-by-single-cell-rnaseq","status":"publish","type":"post","link":"https:\/\/inmuno.es\/index.php\/2025\/08\/02\/genomic-specificity-of-anti-mouse-tcr-mabs-determined-by-single-cell-rnaseq\/","title":{"rendered":"Genomic specificity of anti-mouse TCR mAbs determined by single-cell RNAseq"},"content":{"rendered":"<div>\n<p><b>J Immunol<\/b>. 2025 Aug 1:vkaf094. doi: 10.1093\/jimmun\/vkaf094. Online ahead of print.<\/p>\n<p><b>ABSTRACT<\/b><\/p>\n<p>T cells play a pivotal role in the immune system, relying on their somatically rearranged T cell receptor (TCR) to recognize peptide-MHC complexes. A comprehensive and extensively used set of monoclonal antibodies (mAbs) against TCR variable regions was generated in the previous century. The separate identification of mAb-specific TCR-V proteins and TRV genes has resulted in multiple nomenclatures, making their relationships unclear. To formally re-establish this link and determine patterns of reactivity within TRV subfamilies, we sorted T cells from C57BL\/6 mice positive for any one of a panel of 22 anti-V mAbs and determined their TRV genes by single-cell TCRseq. RNAseq data revealed consistently higher expression of repeated elements from the ERV1-family LTR RLTR6Mm (mapping to Gm20400) in cells utilizing TRBV segments encoded within a 66 kb genomic region between TRBV23 and TRBV30. Our findings provide a comprehensive resource for anti-mouse TCR mAb specificity and insight into V-gene usage biases and T cell function.<\/p>\n<p>PMID:<a href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/40751448\/?utm_source=SimplePie&amp;utm_medium=rss&amp;utm_content=2985117R&amp;ff=20250802104720&amp;v=2.18.0.post9+e462414\">40751448<\/a> | DOI:<a href=\"https:\/\/doi.org\/10.1093\/jimmun\/vkaf094\">10.1093\/jimmun\/vkaf094<\/a><\/p>\n<\/div>","protected":false},"excerpt":{"rendered":"<p>J Immunol. 2025 Aug 1:vkaf094. doi: 10.1093\/jimmun\/vkaf094. Online ahead of print. ABSTRACT T cells play a pivotal role in the immune system, relying on their somatically rearranged T cell receptor (TCR) to recognize peptide-MHC complexes. A comprehensive and extensively used set of monoclonal antibodies (mAbs) against TCR variable regions was generated in the previous century. &#8230; <a title=\"Genomic specificity of anti-mouse TCR mAbs determined by single-cell RNAseq\" class=\"read-more\" href=\"https:\/\/inmuno.es\/index.php\/2025\/08\/02\/genomic-specificity-of-anti-mouse-tcr-mabs-determined-by-single-cell-rnaseq\/\" aria-label=\"Read more about Genomic specificity of anti-mouse TCR mAbs determined by single-cell RNAseq\">Read more<\/a><\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[42,71],"tags":[],"class_list":["post-39538","post","type-post","status-publish","format-standard","hentry","category-publicaciones","category-the-journal-of-immunology"],"_links":{"self":[{"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/posts\/39538","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/comments?post=39538"}],"version-history":[{"count":0,"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/posts\/39538\/revisions"}],"wp:attachment":[{"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/media?parent=39538"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/categories?post=39538"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/tags?post=39538"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}