{"id":40322,"date":"2025-08-13T00:47:12","date_gmt":"2025-08-12T22:47:12","guid":{"rendered":"https:\/\/inmuno.es\/index.php\/2025\/08\/13\/ipsc-derived-macrophages-an-in-vitro-model-to-study-human-disease-relevant-macrophage-biology\/"},"modified":"2025-08-13T00:47:12","modified_gmt":"2025-08-12T22:47:12","slug":"ipsc-derived-macrophages-an-in-vitro-model-to-study-human-disease-relevant-macrophage-biology","status":"publish","type":"post","link":"https:\/\/inmuno.es\/index.php\/2025\/08\/13\/ipsc-derived-macrophages-an-in-vitro-model-to-study-human-disease-relevant-macrophage-biology\/","title":{"rendered":"iPSC-derived macrophages: An in vitro model to study human disease-relevant macrophage biology"},"content":{"rendered":"<div>\n<p><b>J Immunol<\/b>. 2025 Aug 7:vkaf184. doi: 10.1093\/jimmun\/vkaf184. Online ahead of print.<\/p>\n<p><b>ABSTRACT<\/b><\/p>\n<p>Human macrophages differ from their mouse counterparts in multiple metabolic pathways, surface protein expression, and transcription factor biology. Monocyte-derived macrophages (MDMs) from blood are generally used to study human macrophage biology in vitro. However, the use of MDMs as a human macrophage model is limited by donor-to-donor variability, total cell availability, preactivation effects, and relative resistance to genetic manipulation. Here, we explored stem cell-derived macrophages to study in vitro disease-relevant macrophage phenotypes. We compared induced pluripotent stem cell-derived macrophages (iDMs) with primary human stem cell-derived macrophages from cord blood-derived macrophages or bone marrow-derived macrophages (BMDMs) in their ability to elaborate tissue and disease-relevant phenotypes in comparison with conventional blood MDMs. Analyses of phenotypical and functional features revealed that iDMs were highly similar to cord blood-derived macrophages and BMDMs, and resembled MDMs. Importantly, all macrophages, and particularly iDMs adopted expected disease-relevant phenotypes in vitro when stimulated with disease-associated mediators. RNA sequencing analyses of in vitro macrophages stimulated with tissue relevant factors revealed that their transcriptomes mapped to human in vivo inflammatory conditions. Using this approach, we found a close correlation between stimulation and expected disease-association counterpart in vivo. In conclusion, we established a streamlined system to link stem cell-derived macrophage phenotypes to relevant macrophage phenotypes in disease states. Thus, enabling conditions for in vitro replication of complex human macrophage biology observed in single-cell RNA sequencing from human diseased tissue.<\/p>\n<p>PMID:<a href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/40795241\/?utm_source=SimplePie&amp;utm_medium=rss&amp;utm_content=2985117R&amp;ff=20250812184709&amp;v=2.18.0.post9+e462414\">40795241<\/a> | DOI:<a href=\"https:\/\/doi.org\/10.1093\/jimmun\/vkaf184\">10.1093\/jimmun\/vkaf184<\/a><\/p>\n<\/div>","protected":false},"excerpt":{"rendered":"<p>J Immunol. 2025 Aug 7:vkaf184. doi: 10.1093\/jimmun\/vkaf184. Online ahead of print. ABSTRACT Human macrophages differ from their mouse counterparts in multiple metabolic pathways, surface protein expression, and transcription factor biology. Monocyte-derived macrophages (MDMs) from blood are generally used to study human macrophage biology in vitro. However, the use of MDMs as a human macrophage model &#8230; <a title=\"iPSC-derived macrophages: An in vitro model to study human disease-relevant macrophage biology\" class=\"read-more\" href=\"https:\/\/inmuno.es\/index.php\/2025\/08\/13\/ipsc-derived-macrophages-an-in-vitro-model-to-study-human-disease-relevant-macrophage-biology\/\" aria-label=\"Read more about iPSC-derived macrophages: An in vitro model to study human disease-relevant macrophage biology\">Read more<\/a><\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[42,71],"tags":[],"class_list":["post-40322","post","type-post","status-publish","format-standard","hentry","category-publicaciones","category-the-journal-of-immunology"],"_links":{"self":[{"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/posts\/40322","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/comments?post=40322"}],"version-history":[{"count":0,"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/posts\/40322\/revisions"}],"wp:attachment":[{"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/media?parent=40322"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/categories?post=40322"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/tags?post=40322"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}