![\"Mast](\"https:\/\/onlinelibrary.wiley.com\/cms\/asset\/fbd2ac48-b29c-45d4-956f-16ecbebea057\/eji70040-gra-0001-m.png\")
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Mast cell phenotypic features influence anti-bacterial response. Connective tissue-like mast cells sense Salmonella<\/i> through combined Toll-like-receptor (TLR) signaling and recognition of Type-III-secretion-system mediated invasion. By contrast, due to low TLR expression, mucosal-like mast cells fail to respond to extracellular Salmonella<\/i>, but their abundant granules limit intracellular colonization by invasive bacteria. <\/p>\n
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Mast cells (MCs) lodge within barrier tissues and respond to infectious microbes. Recent work demonstrated that MCs differentiate their cytokine response to extracellular versus invasive Gram-negative enterobacteria by a two-step activation mechanism that integrates Toll-like-receptor (TLR) sensing with signals elicited by type-III-secretion-system (TTSS) effectors during bacterial invasion. However, multiple MC subtypes exist, and it remains unclear how their phenotypic heterogeneity impacts microbial interactions. We find that murine MCs maintained in IL-3, or differentiated toward a connective-tissue phenotype (CT-MCs), respond potently to the enteropathogen Salmonella enterica<\/i> Typhimurium (S<\/i>.Tm) through two-step activation, with the TLR component explained by functional TLR4 and TLR2. By contrast, murine mucosal mast cells (M-MCs) express insignificant levels of these TLRs, therefore being unresponsive to extracellular S<\/i>.Tm, but still mounting a response to invasive bacteria. Following invasion, MC granule maintenance by serglycin restricts S<\/i>.Tm vacuolar and cytosolic colonization. Notably, this has no impact on the cytokine release from infected MCs, thus uncoupling S<\/i>.Tm\u00b4s intracellular life-cycle from the MC cytokine response. Finally, human LUVA MCs employ a variant of two-step activation where TLR2\/6 signaling combines with the TTSS-elicited signals. Together, this study explains how MC subtypes can respond differently to S<\/i>.Tm-infection depending on their TLR expression and granule features.<\/p>","protected":false},"excerpt":{"rendered":"
Mast cell phenotypic features influence anti-bacterial response. Connective tissue-like mast cells sense Salmonella through combined Toll-like-receptor (TLR) signaling and recognition of Type-III-secretion-system mediated invasion. By contrast, due to low TLR expression, mucosal-like mast cells fail to respond to extracellular Salmonella, but their abundant granules limit intracellular colonization by invasive bacteria. ABSTRACT Mast cells (MCs) lodge … Read more<\/a><\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[112,42],"tags":[],"class_list":["post-41376","post","type-post","status-publish","format-standard","hentry","category-european-journal-of-immunology","category-publicaciones"],"_links":{"self":[{"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/posts\/41376","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/comments?post=41376"}],"version-history":[{"count":0,"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/posts\/41376\/revisions"}],"wp:attachment":[{"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/media?parent=41376"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/categories?post=41376"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/tags?post=41376"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}