{"id":43557,"date":"2025-09-17T09:51:39","date_gmt":"2025-09-17T07:51:39","guid":{"rendered":"https:\/\/inmuno.es\/index.php\/2025\/09\/17\/efficient-expression-and-purification-of-recombinant-mouse-dimeric-iga-valueantonia-geisse-tao-zhang-jonathan-schreiber-kristina-markova-sophie-burkhalter-hedda-wardemann-andrew\/"},"modified":"2025-09-17T09:51:39","modified_gmt":"2025-09-17T07:51:39","slug":"efficient-expression-and-purification-of-recombinant-mouse-dimeric-iga-valueantonia-geisse-tao-zhang-jonathan-schreiber-kristina-markova-sophie-burkhalter-hedda-wardemann-andrew","status":"publish","type":"post","link":"https:\/\/inmuno.es\/index.php\/2025\/09\/17\/efficient-expression-and-purification-of-recombinant-mouse-dimeric-iga-valueantonia-geisse-tao-zhang-jonathan-schreiber-kristina-markova-sophie-burkhalter-hedda-wardemann-andrew\/","title":{"rendered":"Efficient Expression and Purification of Recombinant Mouse Dimeric IgA. [[{&#8220;value&#8221;:&#8221;Antonia Geisse, \nTao Zhang, \nJonathan Schreiber, \nKristina Markova, \nSophie Burkhalter, \nHedda Wardemann, \nAndrew J. Macpherson, \nTim Rollenske&#8221;}]]"},"content":{"rendered":"<p><img decoding=\"async\" src=\"https:\/\/onlinelibrary.wiley.com\/cms\/asset\/b9f11897-a781-4245-9e21-bd43521608d7\/eji70055-gra-0001-m.png\" alt=\"Efficient Expression and Purification of Recombinant Mouse Dimeric IgA\" \/><\/p>\n<p>Immunoglobulin (Ig) A is the main antibody isotype found on mucosal surfaces in mammals, where it is predominantly present as a dimer. Here we provide an easy, scalable, efficient, and broadly applicable method to produce and purify monoclonal mouse dimeric IgA from single B cell Ig transcripts to study mucosal antibody responses at single-cell level.<\/p>\n<p><\/p>","protected":false},"excerpt":{"rendered":"<p>Immunoglobulin (Ig) A is the main antibody isotype found on mucosal surfaces in mammals, where it is predominantly present as a dimer. Here we provide an easy, scalable, efficient, and broadly applicable method to produce and purify monoclonal mouse dimeric IgA from single B cell Ig transcripts to study mucosal antibody responses at single-cell level.<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[112,42],"tags":[],"class_list":["post-43557","post","type-post","status-publish","format-standard","hentry","category-european-journal-of-immunology","category-publicaciones"],"_links":{"self":[{"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/posts\/43557","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/comments?post=43557"}],"version-history":[{"count":0,"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/posts\/43557\/revisions"}],"wp:attachment":[{"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/media?parent=43557"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/categories?post=43557"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/tags?post=43557"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}