{"id":44146,"date":"2025-09-26T07:02:07","date_gmt":"2025-09-26T05:02:07","guid":{"rendered":"https:\/\/inmuno.es\/index.php\/2025\/09\/26\/the-b-cell-subtypes-and-vdj-repertoire-of-young-adult-rhesus-macaques\/"},"modified":"2025-09-26T07:02:07","modified_gmt":"2025-09-26T05:02:07","slug":"the-b-cell-subtypes-and-vdj-repertoire-of-young-adult-rhesus-macaques","status":"publish","type":"post","link":"https:\/\/inmuno.es\/index.php\/2025\/09\/26\/the-b-cell-subtypes-and-vdj-repertoire-of-young-adult-rhesus-macaques\/","title":{"rendered":"The B cell subtypes and VDJ repertoire of young adult rhesus macaques"},"content":{"rendered":"<div>\n<p><b>J Immunol<\/b>. 2025 Sep 25:vkaf212. doi: 10.1093\/jimmun\/vkaf212. Online ahead of print.<\/p>\n<p><b>ABSTRACT<\/b><\/p>\n<p>Rhesus macaques (RMs) are widely employed as a preclinical model in vaccination and infectious disease studies, yet their B cell immunobiology and immunogenetics remain ill-characterized. In this study, single-cell RNA\/VDJ sequencing was conducted on peripheral blood mononuclear cell samples from 6 RMs to describe the transcriptomic and V(D)JC repertoires of B cells and subtypes. Twelve RM B cell clusters of distinct transcriptional states were identified, including IgM+ memory B cells (MBCs), class-switched MBCs, CD11c+ MBCs, and activated B cells. Novel gene signatures were also characterized for each B cell subtype, such as FCRL2 and CD24 for circulating marginal zone-like B cells. In addition, VDJ repertoire properties of the global B cell population and each B cell subtype were elucidated, including IGH\/K\/L-V(D)JC gene family and subtype usage, class-switch recombination status, somatic hypermutation rate and levels, CDRH3 amino acid length, and CDRH3 amino acid hydrophobicity scores. Interesting insights included the 1:1 ratio of kappa and lambda light chain usage and a preferential decreased IGHV3 but increased IGHV1 and 5 gene family usage in IGHG1 than IGHM-bearing B cells. Altogether, this study through comprehensive transcriptomic analyses identifies 12 distinct RM B cell subtypes paired with their respective V(D)JC repertoire, unraveling the complexity of B cell heterogeneity and improving future preclinical studies that can translate insights from this important nonhuman primate model to the understanding of human immunobiology.<\/p>\n<p>PMID:<a href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/40998330\/?utm_source=SimplePie&amp;utm_medium=rss&amp;utm_content=2985117R&amp;ff=20250926010206&amp;v=2.18.0.post9+e462414\">40998330<\/a> | DOI:<a href=\"https:\/\/doi.org\/10.1093\/jimmun\/vkaf212\">10.1093\/jimmun\/vkaf212<\/a><\/p>\n<\/div>","protected":false},"excerpt":{"rendered":"<p>J Immunol. 2025 Sep 25:vkaf212. doi: 10.1093\/jimmun\/vkaf212. Online ahead of print. ABSTRACT Rhesus macaques (RMs) are widely employed as a preclinical model in vaccination and infectious disease studies, yet their B cell immunobiology and immunogenetics remain ill-characterized. In this study, single-cell RNA\/VDJ sequencing was conducted on peripheral blood mononuclear cell samples from 6 RMs to &#8230; <a title=\"The B cell subtypes and VDJ repertoire of young adult rhesus macaques\" class=\"read-more\" href=\"https:\/\/inmuno.es\/index.php\/2025\/09\/26\/the-b-cell-subtypes-and-vdj-repertoire-of-young-adult-rhesus-macaques\/\" aria-label=\"Read more about The B cell subtypes and VDJ repertoire of young adult rhesus macaques\">Read more<\/a><\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[42,71],"tags":[],"class_list":["post-44146","post","type-post","status-publish","format-standard","hentry","category-publicaciones","category-the-journal-of-immunology"],"_links":{"self":[{"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/posts\/44146","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/comments?post=44146"}],"version-history":[{"count":0,"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/posts\/44146\/revisions"}],"wp:attachment":[{"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/media?parent=44146"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/categories?post=44146"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/tags?post=44146"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}