{"id":44197,"date":"2025-09-26T18:59:17","date_gmt":"2025-09-26T16:59:17","guid":{"rendered":"https:\/\/inmuno.es\/index.php\/2025\/09\/26\/cd206cd14-skin-resident-macrophages-and-dc-t-cell-clusters-are-spatial-features-characterizing-non-relapsing-cutaneous-squamous-cell-carcinoma\/"},"modified":"2025-09-26T18:59:17","modified_gmt":"2025-09-26T16:59:17","slug":"cd206cd14-skin-resident-macrophages-and-dc-t-cell-clusters-are-spatial-features-characterizing-non-relapsing-cutaneous-squamous-cell-carcinoma","status":"publish","type":"post","link":"https:\/\/inmuno.es\/index.php\/2025\/09\/26\/cd206cd14-skin-resident-macrophages-and-dc-t-cell-clusters-are-spatial-features-characterizing-non-relapsing-cutaneous-squamous-cell-carcinoma\/","title":{"rendered":"CD206+CD14- Skin-resident Macrophages and DC-T Cell clusters are spatial features characterizing non-Relapsing Cutaneous Squamous Cell Carcinoma"},"content":{"rendered":"<div>\n<p><b>Cancer Immunol Res<\/b>. 2025 Sep 26. doi: 10.1158\/2326-6066.CIR-25-0182. Online ahead of print.<\/p>\n<p><b>ABSTRACT<\/b><\/p>\n<p>Current histopathological classifications do not reliably distinguish patients with primary cutaneous squamous cell carcinoma (cSCC) at risk of relapse from those with non-relapsing tumors. This underscores the need for molecular signatures capable of stratifying patients during primary tumor resection. Here, we used high-dimensional imaging mass cytometry (IMC) and a 39-antibody panel to define the immune landscape of twenty primary cSCC with distinct clinical outcomes, four relapsing cSCC and their perilesional skins. Computational analysis of spatially-resolved single-cell data from forty-seven IMC images identified twelve immune-cell subsets that discriminated primary cSCC from perilesional skin. Regulatory T cells, cytotoxic CD8+ T lymphocytes, tumor-associated macrophages and neutrophils characterized tumors while Langerhans cells and skin-resident macrophages defined perilesional skin. Skin-resident macrophages were characterized by the expression of CD206, CD11c and HLA-DR and the absence of CD14. These cells infiltrated tumors from non-relapsing patients more efficiently. We found a higher density of proliferating, mature and cytotoxic cells within this macrophage subset, consistent with the absence of relapse. Spectral flow cytometry analysis on fresh tumor biopsies revealed the skin-resident macrophages had phagocytic properties, suggesting a role in tumor antigen processing. Additionally, neighborhood profiling revealed DC-LAMP+ dendritic cells (DC) were in close proximity with helper and cytotoxic T lymphocytes in primary cSCC from patients without relapse, indicative of active adaptive immunity. Our findings identify phagocytic skin-resident macrophages and DC-T cell clusters as features differentiating non-relapsing cSCC from primary cSCC at risk of relapse. These data have the potential to guide the identification of prognostic biomarkers for cSCC.<\/p>\n<p>PMID:<a href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/41003664\/?utm_source=SimplePie&amp;utm_medium=rss&amp;utm_content=101614637&amp;ff=20250926125916&amp;v=2.18.0.post9+e462414\">41003664<\/a> | DOI:<a href=\"https:\/\/doi.org\/10.1158\/2326-6066.CIR-25-0182\">10.1158\/2326-6066.CIR-25-0182<\/a><\/p>\n<\/div>","protected":false},"excerpt":{"rendered":"<p>Cancer Immunol Res. 2025 Sep 26. doi: 10.1158\/2326-6066.CIR-25-0182. Online ahead of print. ABSTRACT Current histopathological classifications do not reliably distinguish patients with primary cutaneous squamous cell carcinoma (cSCC) at risk of relapse from those with non-relapsing tumors. This underscores the need for molecular signatures capable of stratifying patients during primary tumor resection. Here, we used &#8230; <a title=\"CD206+CD14- Skin-resident Macrophages and DC-T Cell clusters are spatial features characterizing non-Relapsing Cutaneous Squamous Cell Carcinoma\" class=\"read-more\" href=\"https:\/\/inmuno.es\/index.php\/2025\/09\/26\/cd206cd14-skin-resident-macrophages-and-dc-t-cell-clusters-are-spatial-features-characterizing-non-relapsing-cutaneous-squamous-cell-carcinoma\/\" aria-label=\"Read more about CD206+CD14- Skin-resident Macrophages and DC-T Cell clusters are spatial features characterizing non-Relapsing Cutaneous Squamous Cell Carcinoma\">Read more<\/a><\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[55,42],"tags":[],"class_list":["post-44197","post","type-post","status-publish","format-standard","hentry","category-cancer-immunology-reserch","category-publicaciones"],"_links":{"self":[{"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/posts\/44197","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/comments?post=44197"}],"version-history":[{"count":0,"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/posts\/44197\/revisions"}],"wp:attachment":[{"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/media?parent=44197"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/categories?post=44197"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/tags?post=44197"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}