{"id":44438,"date":"2025-10-02T00:48:18","date_gmt":"2025-10-01T22:48:18","guid":{"rendered":"https:\/\/inmuno.es\/index.php\/2025\/10\/02\/dynamic-control-of-autoreactive-b-cells-from-spontaneous-germinal-centers\/"},"modified":"2025-10-02T00:48:18","modified_gmt":"2025-10-01T22:48:18","slug":"dynamic-control-of-autoreactive-b-cells-from-spontaneous-germinal-centers","status":"publish","type":"post","link":"https:\/\/inmuno.es\/index.php\/2025\/10\/02\/dynamic-control-of-autoreactive-b-cells-from-spontaneous-germinal-centers\/","title":{"rendered":"Dynamic control of autoreactive B cells from spontaneous germinal centers"},"content":{"rendered":"<div>\n<p><b>J Immunol<\/b>. 2025 Oct 1:vkaf223. doi: 10.1093\/jimmun\/vkaf223. Online ahead of print.<\/p>\n<p><b>ABSTRACT<\/b><\/p>\n<p>Systemic lupus erythematosus is characterized by activation of many self-reactive B cell clones that produce autoantibodies. This can be modeled using mixed bone marrow chimeras, where autoreactive 564Igi B cells initiate autoimmunity that spreads to wild-type (WT) B cells. The mechanisms controlling the inclusion of new B cell clones into spontaneous germinal centers (GCs) remain unclear. Using CRISPR-Cas9, we generated 2 autoreactive B cell receptor knock-in strains, M05 and G55, based on B cell receptors from WT GC B cells in WT:564Igi chimeras. M05 and G55 mice lacked spontaneous GCs and overt autoantibody production, with receptor editing (\u03bb light chain expression) contributing to tolerance. However, autoreactivity was not purged from the B cell compartment since presence of 564Igi clone allowed M05 and G55 B cells to join GCs and produce autoantibodies. These findings reveal that GCs can override peripheral tolerance, recruiting previously silent autoreactive clones and facilitating diversification of autoantibodies.<\/p>\n<p>PMID:<a href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/41032891\/?utm_source=SimplePie&amp;utm_medium=rss&amp;utm_content=2985117R&amp;ff=20251001184818&amp;v=2.18.0.post9+e462414\">41032891<\/a> | DOI:<a href=\"https:\/\/doi.org\/10.1093\/jimmun\/vkaf223\">10.1093\/jimmun\/vkaf223<\/a><\/p>\n<\/div>","protected":false},"excerpt":{"rendered":"<p>J Immunol. 2025 Oct 1:vkaf223. doi: 10.1093\/jimmun\/vkaf223. Online ahead of print. ABSTRACT Systemic lupus erythematosus is characterized by activation of many self-reactive B cell clones that produce autoantibodies. This can be modeled using mixed bone marrow chimeras, where autoreactive 564Igi B cells initiate autoimmunity that spreads to wild-type (WT) B cells. The mechanisms controlling the &#8230; <a title=\"Dynamic control of autoreactive B cells from spontaneous germinal centers\" class=\"read-more\" href=\"https:\/\/inmuno.es\/index.php\/2025\/10\/02\/dynamic-control-of-autoreactive-b-cells-from-spontaneous-germinal-centers\/\" aria-label=\"Read more about Dynamic control of autoreactive B cells from spontaneous germinal centers\">Read more<\/a><\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[42,71],"tags":[],"class_list":["post-44438","post","type-post","status-publish","format-standard","hentry","category-publicaciones","category-the-journal-of-immunology"],"_links":{"self":[{"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/posts\/44438","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/comments?post=44438"}],"version-history":[{"count":0,"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/posts\/44438\/revisions"}],"wp:attachment":[{"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/media?parent=44438"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/categories?post=44438"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/tags?post=44438"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}