{"id":44935,"date":"2025-10-08T12:49:12","date_gmt":"2025-10-08T10:49:12","guid":{"rendered":"https:\/\/inmuno.es\/index.php\/2025\/10\/08\/human-cd24cd38-regulatory-b-cells-promote-pro-resolving-macrophage-function-through-the-synergistic-effect-of-il-10-and-pd-l1elaheh-dalir-abdolahinia-on-8-de-october-de-2025-at-1000\/"},"modified":"2025-10-08T12:49:12","modified_gmt":"2025-10-08T10:49:12","slug":"human-cd24cd38-regulatory-b-cells-promote-pro-resolving-macrophage-function-through-the-synergistic-effect-of-il-10-and-pd-l1elaheh-dalir-abdolahinia-on-8-de-october-de-2025-at-1000","status":"publish","type":"post","link":"https:\/\/inmuno.es\/index.php\/2025\/10\/08\/human-cd24cd38-regulatory-b-cells-promote-pro-resolving-macrophage-function-through-the-synergistic-effect-of-il-10-and-pd-l1elaheh-dalir-abdolahinia-on-8-de-october-de-2025-at-1000\/","title":{"rendered":"Human CD24+CD38+ regulatory B cells promote Pro-resolving Macrophage Function through the synergistic effect of IL-10 and PD-L1\u200bElaheh Dalir Abdolahinia   on 8 de October de 2025 at 10:00"},"content":{"rendered":"<div>\n<p><b>J Leukoc Biol<\/b>. 2025 Oct 7:qiaf141. doi: 10.1093\/jleuko\/qiaf141. Online ahead of print.<\/p>\n<p><b>ABSTRACT<\/b><\/p>\n<p>Regulatory B cells (Bregs), specifically the CD24\u207aCD38\u207a phenotype, are known for their capacity to reduce inflammation by releasing interleukin 10(IL-10). However, their potential role in regulating inflammation through macrophage differentiation is not well understood. This study investigates how CD24\u207aCD38\u207a regulatory B cells modulate the differentiation and function of pro-resolving macrophages through programmed death-ligand 1 (PD-L1)\/programmed death-1 (PD-1) interactions and IL-10 secretion. Human CD24\u207aCD38\u207a B cells were isolated from peripheral blood and co-cultured with THP-1-derived macrophages. Recombinant IL-10\/PD-L1 and neutralizing antibodies were used to conduct gain and loss-of-function studies. Flow cytometry, quantitative PCR, and mass spectrometry were used to evaluate macrophage polarization, efferocytosis activity, and pro-resolving lipid mediator production. After co-culture, M2 polarization, PD-1 expression, and efferocytosis activity were increased significantly. Inhibition of either IL-10 or PD-L1 pathway reduced M2 macrophage differentiation and functional activity. Co-culture of macrophages with CD24\u207aCD38\u207a B cells enhanced the production of pro-resolving lipid mediators, particularly 12-HEPE and RvD5 through IL-10 secretion and PD-L1\/PD-1 ligation. These findings reveal a novel mechanism by which human CD24\u207aCD38\u207a regulatory B cells promote macrophage-mediated resolution of inflammation through IL-10 secretion and PD-L1\/PD-1 ligation. By exploring how these regulatory pathways influence macrophage biology, we ultimately aim to uncover novel therapeutic targets for enhancing inflammation resolution in chronic inflammatory diseases.<\/p>\n<p>PMID:<a href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/41058100\/?utm_source=SimplePie&amp;utm_medium=rss&amp;utm_content=8405628&amp;ff=20251008064912&amp;v=2.18.0.post9+e462414\">41058100<\/a> | DOI:<a href=\"https:\/\/doi.org\/10.1093\/jleuko\/qiaf141\">10.1093\/jleuko\/qiaf141<\/a><\/p>\n<\/div>","protected":false},"excerpt":{"rendered":"<p>J Leukoc Biol. 2025 Oct 7:qiaf141. doi: 10.1093\/jleuko\/qiaf141. Online ahead of print. ABSTRACT Regulatory B cells (Bregs), specifically the CD24\u207aCD38\u207a phenotype, are known for their capacity to reduce inflammation by releasing interleukin 10(IL-10). However, their potential role in regulating inflammation through macrophage differentiation is not well understood. This study investigates how CD24\u207aCD38\u207a regulatory B cells &#8230; <a title=\"Human CD24+CD38+ regulatory B cells promote Pro-resolving Macrophage Function through the synergistic effect of IL-10 and PD-L1\u200bElaheh Dalir Abdolahinia   on 8 de October de 2025 at 10:00\" class=\"read-more\" href=\"https:\/\/inmuno.es\/index.php\/2025\/10\/08\/human-cd24cd38-regulatory-b-cells-promote-pro-resolving-macrophage-function-through-the-synergistic-effect-of-il-10-and-pd-l1elaheh-dalir-abdolahinia-on-8-de-october-de-2025-at-1000\/\" aria-label=\"Read more about Human CD24+CD38+ regulatory B cells promote Pro-resolving Macrophage Function through the synergistic effect of IL-10 and PD-L1\u200bElaheh Dalir Abdolahinia   on 8 de October de 2025 at 10:00\">Read more<\/a><\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[86,42],"tags":[],"class_list":["post-44935","post","type-post","status-publish","format-standard","hentry","category-journal-of-leukocyte-biology","category-publicaciones"],"_links":{"self":[{"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/posts\/44935","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/comments?post=44935"}],"version-history":[{"count":0,"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/posts\/44935\/revisions"}],"wp:attachment":[{"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/media?parent=44935"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/categories?post=44935"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/tags?post=44935"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}