{"id":46860,"date":"2025-10-27T20:49:33","date_gmt":"2025-10-27T19:49:33","guid":{"rendered":"https:\/\/inmuno.es\/index.php\/2025\/10\/27\/altered-thymopoiesis-in-thymoma-is-associated-with-defects-in-negative-selection-machinery-and-decreased-treg-abundance\/"},"modified":"2025-10-27T20:49:33","modified_gmt":"2025-10-27T19:49:33","slug":"altered-thymopoiesis-in-thymoma-is-associated-with-defects-in-negative-selection-machinery-and-decreased-treg-abundance","status":"publish","type":"post","link":"https:\/\/inmuno.es\/index.php\/2025\/10\/27\/altered-thymopoiesis-in-thymoma-is-associated-with-defects-in-negative-selection-machinery-and-decreased-treg-abundance\/","title":{"rendered":"Altered thymopoiesis in thymoma is associated with defects in negative selection machinery and decreased Treg abundance"},"content":{"rendered":"<div>\n<p><b>Cancer Immunol Res<\/b>. 2025 Oct 27. doi: 10.1158\/2326-6066.CIR-25-0190. Online ahead of print.<\/p>\n<p><b>ABSTRACT<\/b><\/p>\n<p>Thymomas are rare thymic epithelial tumors harboring a high but variable proportion of lymphocytes without obvious function. Autoimmunity is present in one third of patients at diagnosis. Herein, we performed a phenotypic, single-cell RNA sequencing (scRNAseq), and spatial analysis of both the T cells and tumoral cells. T cells at all stages of T-cell development-from immature to mature-were present in the tumor suggesting active thymopoiesis in thymoma. However, data generated through multiple approaches suggested a maturation blockade at the double negative to double positive stage of T-cell development. In the mature T-cell compartment, the frequency of regulatory T cells was strongly decreased. The scRNAseq analysis showed that the transcriptome of tumoral Thymic Epithelial Cells (tTEC) was most similar to that of non-tumoral medullary TEC but the expression of key molecules involved in positive and negative selection was defective. Multiplexed Immunohistochemical Consecutive Staining revealed a loss of the cortex-medulla zoning in thymoma, which may be related to a decrease in the expression of T cell-targeted chemokines by tTEC. Altogether, these results suggest that the thymopoiesis present in thymoma is abnormal and may be the cause of the prevalent autoimmunity observed in this disease.<\/p>\n<p>PMID:<a href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/41143696\/?utm_source=SimplePie&amp;utm_medium=rss&amp;utm_content=101614637&amp;ff=20251027154932&amp;v=2.18.0.post22+67771e2\">41143696<\/a> | DOI:<a href=\"https:\/\/doi.org\/10.1158\/2326-6066.CIR-25-0190\">10.1158\/2326-6066.CIR-25-0190<\/a><\/p>\n<\/div>","protected":false},"excerpt":{"rendered":"<p>Cancer Immunol Res. 2025 Oct 27. doi: 10.1158\/2326-6066.CIR-25-0190. Online ahead of print. ABSTRACT Thymomas are rare thymic epithelial tumors harboring a high but variable proportion of lymphocytes without obvious function. Autoimmunity is present in one third of patients at diagnosis. Herein, we performed a phenotypic, single-cell RNA sequencing (scRNAseq), and spatial analysis of both the &#8230; <a title=\"Altered thymopoiesis in thymoma is associated with defects in negative selection machinery and decreased Treg abundance\" class=\"read-more\" href=\"https:\/\/inmuno.es\/index.php\/2025\/10\/27\/altered-thymopoiesis-in-thymoma-is-associated-with-defects-in-negative-selection-machinery-and-decreased-treg-abundance\/\" aria-label=\"Read more about Altered thymopoiesis in thymoma is associated with defects in negative selection machinery and decreased Treg abundance\">Read more<\/a><\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[55,42],"tags":[],"class_list":["post-46860","post","type-post","status-publish","format-standard","hentry","category-cancer-immunology-reserch","category-publicaciones"],"_links":{"self":[{"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/posts\/46860","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/comments?post=46860"}],"version-history":[{"count":0,"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/posts\/46860\/revisions"}],"wp:attachment":[{"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/media?parent=46860"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/categories?post=46860"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/tags?post=46860"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}