{"id":47744,"date":"2025-11-07T18:48:49","date_gmt":"2025-11-07T17:48:49","guid":{"rendered":"https:\/\/inmuno.es\/index.php\/2025\/11\/07\/intratumoral-three-cell-type-clusters-are-a-conserved-feature-of-endogenous-antitumor-immunity\/"},"modified":"2025-11-07T18:48:49","modified_gmt":"2025-11-07T17:48:49","slug":"intratumoral-three-cell-type-clusters-are-a-conserved-feature-of-endogenous-antitumor-immunity","status":"publish","type":"post","link":"https:\/\/inmuno.es\/index.php\/2025\/11\/07\/intratumoral-three-cell-type-clusters-are-a-conserved-feature-of-endogenous-antitumor-immunity\/","title":{"rendered":"Intratumoral three-cell-type clusters are a conserved feature of endogenous antitumor immunity"},"content":{"rendered":"<div>\n<p><b>Cancer Immunol Res<\/b>. 2025 Nov 7. doi: 10.1158\/2326-6066.CIR-25-0062. Online ahead of print.<\/p>\n<p><b>ABSTRACT<\/b><\/p>\n<p>Effective antitumor immunity ultimately depends on the priming and activation of tumor-specific cytotoxic CD8+ T cells; however, the role of intratumoral cell-cell immune interactions remains incompletely understood. Recent work has revealed that the temporospatial colocalization of dendritic cells (DCs), helper T cells (Th), and cytotoxic T lymphocytes (CTL) within the tumor immune microenvironment following immune checkpoint blockade correlates with clinical response. Herein, we report the integration of more than one million spatially resolved single-cell profiles across six spatial proteomic and transcriptomic assays, which demonstrated that DC:Th:CTL three-cell-type clusters were common even in immunotherapy-na\u00efve and highly desmoplastic tumors, such as fibrolamellar carcinoma and pancreatic ductal adenocarcinoma (PDAC). We found that these immune triads were enriched for functionally important type 1 conventional DC, mature DCs enriched in immunoregulatory molecules (mregDC), CXCL13+ Th, and GZMK+ effector CTL phenotypes. Subsequent multiplex immunofluorescence imaging of more than 450 primary PDAC tumors showed that the density of antigen-presenting cell (APC):Th:CTL three-cell-type clusters was correlated with intratumoral T-cell clonal expansion and improved overall survival. These findings suggest that DC:Th:CTL triads are conserved across solid tumors and highlight the importance of intratumoral spatial niches in mediating endogenous antitumor immunity.<\/p>\n<p>PMID:<a href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/41201469\/?utm_source=SimplePie&amp;utm_medium=rss&amp;utm_content=101614637&amp;ff=20251107124848&amp;v=2.18.0.post22+67771e2\">41201469<\/a> | DOI:<a href=\"https:\/\/doi.org\/10.1158\/2326-6066.CIR-25-0062\">10.1158\/2326-6066.CIR-25-0062<\/a><\/p>\n<\/div>","protected":false},"excerpt":{"rendered":"<p>Cancer Immunol Res. 2025 Nov 7. doi: 10.1158\/2326-6066.CIR-25-0062. Online ahead of print. ABSTRACT Effective antitumor immunity ultimately depends on the priming and activation of tumor-specific cytotoxic CD8+ T cells; however, the role of intratumoral cell-cell immune interactions remains incompletely understood. Recent work has revealed that the temporospatial colocalization of dendritic cells (DCs), helper T cells &#8230; <a title=\"Intratumoral three-cell-type clusters are a conserved feature of endogenous antitumor immunity\" class=\"read-more\" href=\"https:\/\/inmuno.es\/index.php\/2025\/11\/07\/intratumoral-three-cell-type-clusters-are-a-conserved-feature-of-endogenous-antitumor-immunity\/\" aria-label=\"Read more about Intratumoral three-cell-type clusters are a conserved feature of endogenous antitumor immunity\">Read more<\/a><\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[55,42],"tags":[],"class_list":["post-47744","post","type-post","status-publish","format-standard","hentry","category-cancer-immunology-reserch","category-publicaciones"],"_links":{"self":[{"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/posts\/47744","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/comments?post=47744"}],"version-history":[{"count":0,"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/posts\/47744\/revisions"}],"wp:attachment":[{"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/media?parent=47744"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/categories?post=47744"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/tags?post=47744"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}