{"id":47781,"date":"2025-11-09T12:48:07","date_gmt":"2025-11-09T11:48:07","guid":{"rendered":"https:\/\/inmuno.es\/index.php\/2025\/11\/09\/method-for-generation-and-ex-vivo-expansion-of-genetically-edited-mouse-tregsyosef-gilad-on-9-de-november-de-2025-at-1100\/"},"modified":"2025-11-09T12:48:07","modified_gmt":"2025-11-09T11:48:07","slug":"method-for-generation-and-ex-vivo-expansion-of-genetically-edited-mouse-tregsyosef-gilad-on-9-de-november-de-2025-at-1100","status":"publish","type":"post","link":"https:\/\/inmuno.es\/index.php\/2025\/11\/09\/method-for-generation-and-ex-vivo-expansion-of-genetically-edited-mouse-tregsyosef-gilad-on-9-de-november-de-2025-at-1100\/","title":{"rendered":"Method for generation and ex vivo expansion of genetically edited mouse Tregs\u200bYosef Gilad   on 9 de November de 2025 at 11:00"},"content":{"rendered":"<div>\n<p><b>J Leukoc Biol<\/b>. 2025 Nov 7:qiaf160. doi: 10.1093\/jleuko\/qiaf160. Online ahead of print.<\/p>\n<p><b>ABSTRACT<\/b><\/p>\n<p>Regulatory T cells (Tregs) play a crucial role in the immune system, and their dysfunction can lead to the development of autoimmune conditions. In cancer, tumors frequently hijack the immunosuppressive function of Tregs to evade immune responses. Due to their central role in key pathological processes, Tregs have gained increasing attention as promising targets for various clinical applications. However, their relative scarcity (\u223c5-10% of CD4+ T cells) and instability presents a technical challenge for research and therapeutic development. In congenic animal models used to investigate autologous cell transfer based therapies, this challenge is even greater, as Treg donor animals may only be able to provide cells to a small number of recipient mice. Here we present an optimized protocol for ex vivo editing and expansion of mouse Tregs (mTregs). Since a recent study demonstrated the anti-cancer potential of SRC-3 KO mTregs, we use them here as a case study.<\/p>\n<p>PMID:<a href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/41206685\/?utm_source=SimplePie&amp;utm_medium=rss&amp;utm_content=8405628&amp;ff=20251109064806&amp;v=2.18.0.post22+67771e2\">41206685<\/a> | DOI:<a href=\"https:\/\/doi.org\/10.1093\/jleuko\/qiaf160\">10.1093\/jleuko\/qiaf160<\/a><\/p>\n<\/div>","protected":false},"excerpt":{"rendered":"<p>J Leukoc Biol. 2025 Nov 7:qiaf160. doi: 10.1093\/jleuko\/qiaf160. Online ahead of print. ABSTRACT Regulatory T cells (Tregs) play a crucial role in the immune system, and their dysfunction can lead to the development of autoimmune conditions. In cancer, tumors frequently hijack the immunosuppressive function of Tregs to evade immune responses. Due to their central role &#8230; <a title=\"Method for generation and ex vivo expansion of genetically edited mouse Tregs\u200bYosef Gilad   on 9 de November de 2025 at 11:00\" class=\"read-more\" href=\"https:\/\/inmuno.es\/index.php\/2025\/11\/09\/method-for-generation-and-ex-vivo-expansion-of-genetically-edited-mouse-tregsyosef-gilad-on-9-de-november-de-2025-at-1100\/\" aria-label=\"Read more about Method for generation and ex vivo expansion of genetically edited mouse Tregs\u200bYosef Gilad   on 9 de November de 2025 at 11:00\">Read more<\/a><\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[86,42],"tags":[],"class_list":["post-47781","post","type-post","status-publish","format-standard","hentry","category-journal-of-leukocyte-biology","category-publicaciones"],"_links":{"self":[{"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/posts\/47781","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/comments?post=47781"}],"version-history":[{"count":0,"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/posts\/47781\/revisions"}],"wp:attachment":[{"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/media?parent=47781"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/categories?post=47781"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/tags?post=47781"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}