{"id":47782,"date":"2025-11-09T12:48:07","date_gmt":"2025-11-09T11:48:07","guid":{"rendered":"https:\/\/inmuno.es\/index.php\/2025\/11\/09\/a-critical-role-of-the-erk1-2-downstream-targets-c-fos-egr-1-and-egr-2-in-pu-1-driven-monocyte-developmenthafiza-sidra-bashir-on-9-de-november-de-2025-at-1100\/"},"modified":"2025-11-09T12:48:07","modified_gmt":"2025-11-09T11:48:07","slug":"a-critical-role-of-the-erk1-2-downstream-targets-c-fos-egr-1-and-egr-2-in-pu-1-driven-monocyte-developmenthafiza-sidra-bashir-on-9-de-november-de-2025-at-1100","status":"publish","type":"post","link":"https:\/\/inmuno.es\/index.php\/2025\/11\/09\/a-critical-role-of-the-erk1-2-downstream-targets-c-fos-egr-1-and-egr-2-in-pu-1-driven-monocyte-developmenthafiza-sidra-bashir-on-9-de-november-de-2025-at-1100\/","title":{"rendered":"A Critical Role of the Erk1\/2 Downstream Targets c-Fos, Egr-1 and Egr-2 in PU.1-Driven Monocyte Development\u200bHafiza Sidra Bashir   on 9 de November de 2025 at 11:00"},"content":{"rendered":"<div>\n<p><b>J Leukoc Biol<\/b>. 2025 Nov 7:qiaf159. doi: 10.1093\/jleuko\/qiaf159. Online ahead of print.<\/p>\n<p><b>ABSTRACT<\/b><\/p>\n<p>The Ets family transcription factor PU.1 plays multiple roles in hematopoiesis. PU.1 acts in a graded manner to regulate myeloid and lymphoid development with high expression levels promoting monopoiesis and low expression levels instructing B cell fate. It remains incompletely understood how PU.1 promotes monocyte development. In this study, we show that PU.1 activation upregulated, whereas PU.1 knockdown downregulated, the expression of c-Fos, Egr-1 and Egr-2, which are the immediate early response genes of the Erk1\/2 pathway and have been shown to promote monopoiesis. A PU.1 mutant defective in c-Jun interaction was unable to upregulate the expression of c-Fos, Egr-1 and Egr-2, indicating that interaction with c-Jun is required for PU.1-mediated upregulation of c-Fos, Egr-1 and Egr-2. We further demonstrate that PU.1 bound to and transcriptionally activated the promoters of c-Fos, Egr-1 and Egr-2. Knockdown of c-Fos or Egr-1 favored neutrophil over monocyte development in response to PU.1 activation, which was associated with increased Gfi1 expression. Notably, PU.1-mediated upregulation of c-Fos, Egr-1 and Egr-2 was blocked upon inhibition of Erk1\/2 signaling, which was also associated with an increase in Gfi1 expression and a shift toward neutrophil development at the expense of monopoiesis. Together, these results reveal a novel mechanism by which PU.1 acts to promote monopoiesis and a critical role of the Erk1\/2 downstream targets c-Fos, Egr-1 and Egr-2 in PU.1-driven monocyte development.<\/p>\n<p>PMID:<a href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/41206684\/?utm_source=SimplePie&amp;utm_medium=rss&amp;utm_content=8405628&amp;ff=20251109064806&amp;v=2.18.0.post22+67771e2\">41206684<\/a> | DOI:<a href=\"https:\/\/doi.org\/10.1093\/jleuko\/qiaf159\">10.1093\/jleuko\/qiaf159<\/a><\/p>\n<\/div>","protected":false},"excerpt":{"rendered":"<p>J Leukoc Biol. 2025 Nov 7:qiaf159. doi: 10.1093\/jleuko\/qiaf159. Online ahead of print. ABSTRACT The Ets family transcription factor PU.1 plays multiple roles in hematopoiesis. PU.1 acts in a graded manner to regulate myeloid and lymphoid development with high expression levels promoting monopoiesis and low expression levels instructing B cell fate. It remains incompletely understood how &#8230; <a title=\"A Critical Role of the Erk1\/2 Downstream Targets c-Fos, Egr-1 and Egr-2 in PU.1-Driven Monocyte Development\u200bHafiza Sidra Bashir   on 9 de November de 2025 at 11:00\" class=\"read-more\" href=\"https:\/\/inmuno.es\/index.php\/2025\/11\/09\/a-critical-role-of-the-erk1-2-downstream-targets-c-fos-egr-1-and-egr-2-in-pu-1-driven-monocyte-developmenthafiza-sidra-bashir-on-9-de-november-de-2025-at-1100\/\" aria-label=\"Read more about A Critical Role of the Erk1\/2 Downstream Targets c-Fos, Egr-1 and Egr-2 in PU.1-Driven Monocyte Development\u200bHafiza Sidra Bashir   on 9 de November de 2025 at 11:00\">Read more<\/a><\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[86,42],"tags":[],"class_list":["post-47782","post","type-post","status-publish","format-standard","hentry","category-journal-of-leukocyte-biology","category-publicaciones"],"_links":{"self":[{"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/posts\/47782","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/comments?post=47782"}],"version-history":[{"count":0,"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/posts\/47782\/revisions"}],"wp:attachment":[{"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/media?parent=47782"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/categories?post=47782"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/tags?post=47782"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}