{"id":47785,"date":"2025-11-09T20:47:14","date_gmt":"2025-11-09T19:47:14","guid":{"rendered":"https:\/\/inmuno.es\/index.php\/2025\/11\/09\/the-involvement-of-clec-htm-igr-syk-bcl10-mtor-erk-signaling-pathway-in-immune-priming-of-oysters\/"},"modified":"2025-11-09T20:47:14","modified_gmt":"2025-11-09T19:47:14","slug":"the-involvement-of-clec-htm-igr-syk-bcl10-mtor-erk-signaling-pathway-in-immune-priming-of-oysters","status":"publish","type":"post","link":"https:\/\/inmuno.es\/index.php\/2025\/11\/09\/the-involvement-of-clec-htm-igr-syk-bcl10-mtor-erk-signaling-pathway-in-immune-priming-of-oysters\/","title":{"rendered":"The involvement of CLec-HTM\/IgR-Syk-BCL10-mTOR\/ERK signaling pathway in immune priming of oysters"},"content":{"rendered":"<div>\n<p><b>J Immunol<\/b>. 2025 Nov 6:vkaf218. doi: 10.1093\/jimmun\/vkaf218. Online ahead of print.<\/p>\n<p><b>ABSTRACT<\/b><\/p>\n<p>Increasing evidence indicates that invertebrates have the immune adaptive abilities, which is commonly known as &#8220;immune priming&#8221;. However, the mechanism of immune priming is believed to be rather complicated, and it has not been fully elucidated. In the present study, in the oysters where the CgCLec-HTM\/IgR-Syk-BCL10 signaling pathway was interfered by silencing the expression of the main components, the phosphorylation of mTOR and ERK decreased significantly at 6 h after the first Vibrio splendidus stimulation. The messenger RNA (mRNA) transcript levels of the glycolysis-associated genes (CgGluT and CgGK), NAD+\/NADH ratio, lactate concentration, and H3K4me1 and H3K4me3 levels all decreased significantly at 7 d after the first V. splendidus stimulation. Meanwhile, the hemocyte phagocytic rates and the mRNA transcript levels of CgClathrin, CgATPeV, and CgTNF also decreased significantly at 7 d after the first stimulation and 6 h after the second stimulation with V. splendidus. The mRNA transcript levels of CgGluT, CgGK, CgATPeV, and CgTNF; lactate concentration; and H3K4me1 and H3K4me3 levels were all reduced in rapamycin-treated oysters after the first stimulation. The mRNA transcript levels of CgGK, CgClathrin, CgATPeV, and CgTNF; NAD+\/NADH ratio; and the levels of H3K4me1 and H3K4me3 were also reduced in FR180204-treated oysters after the first stimulation. These results indicated that the CLec-HTM\/IgR-Syk-BCL10-mTOR\/ERK signaling pathway regulated histone methylation through mediating the glycolysis to enhance cell phagocytosis and inflammatory response and played a significant regulatory role in the immune priming of Crassostrea gigas, which provided new clues for studying the evolution of immune adaptability.<\/p>\n<p>PMID:<a href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/41206721\/?utm_source=SimplePie&amp;utm_medium=rss&amp;utm_content=2985117R&amp;ff=20251109144713&amp;v=2.18.0.post22+67771e2\">41206721<\/a> | DOI:<a href=\"https:\/\/doi.org\/10.1093\/jimmun\/vkaf218\">10.1093\/jimmun\/vkaf218<\/a><\/p>\n<\/div>","protected":false},"excerpt":{"rendered":"<p>J Immunol. 2025 Nov 6:vkaf218. doi: 10.1093\/jimmun\/vkaf218. Online ahead of print. ABSTRACT Increasing evidence indicates that invertebrates have the immune adaptive abilities, which is commonly known as &#8220;immune priming&#8221;. However, the mechanism of immune priming is believed to be rather complicated, and it has not been fully elucidated. In the present study, in the oysters &#8230; <a title=\"The involvement of CLec-HTM\/IgR-Syk-BCL10-mTOR\/ERK signaling pathway in immune priming of oysters\" class=\"read-more\" href=\"https:\/\/inmuno.es\/index.php\/2025\/11\/09\/the-involvement-of-clec-htm-igr-syk-bcl10-mtor-erk-signaling-pathway-in-immune-priming-of-oysters\/\" aria-label=\"Read more about The involvement of CLec-HTM\/IgR-Syk-BCL10-mTOR\/ERK signaling pathway in immune priming of oysters\">Read more<\/a><\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[42,71],"tags":[],"class_list":["post-47785","post","type-post","status-publish","format-standard","hentry","category-publicaciones","category-the-journal-of-immunology"],"_links":{"self":[{"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/posts\/47785","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/comments?post=47785"}],"version-history":[{"count":0,"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/posts\/47785\/revisions"}],"wp:attachment":[{"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/media?parent=47785"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/categories?post=47785"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/tags?post=47785"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}