{"id":48925,"date":"2025-11-17T15:47:39","date_gmt":"2025-11-17T14:47:39","guid":{"rendered":"https:\/\/inmuno.es\/index.php\/2025\/11\/17\/human-m-mdscs-impair-neutrophil-migration-in-the-infectious-microenvironmenthannah-k-weppner-on-17-de-november-de-2025-at-1100\/"},"modified":"2025-11-17T15:47:39","modified_gmt":"2025-11-17T14:47:39","slug":"human-m-mdscs-impair-neutrophil-migration-in-the-infectious-microenvironmenthannah-k-weppner-on-17-de-november-de-2025-at-1100","status":"publish","type":"post","link":"https:\/\/inmuno.es\/index.php\/2025\/11\/17\/human-m-mdscs-impair-neutrophil-migration-in-the-infectious-microenvironmenthannah-k-weppner-on-17-de-november-de-2025-at-1100\/","title":{"rendered":"Human M-MDSCs Impair Neutrophil Migration in the Infectious Microenvironment\u200bHannah K Weppner   on 17 de November de 2025 at 11:00"},"content":{"rendered":"<div>\n<p><b>J Leukoc Biol<\/b>. 2025 Nov 17:qiaf165. doi: 10.1093\/jleuko\/qiaf165. Online ahead of print.<\/p>\n<p><b>ABSTRACT<\/b><\/p>\n<p>Patients who survive sepsis experience a prolonged period of immunosuppression. This period is accompanied by the expansion of monocytic myeloid-derived suppressor cells (M-MDSCs), a subset of suppressive myeloid cells; however, the impact of M-MDSCs on the innate immune response to infection is not well understood. Here we investigate the effect of MDSCs on neutrophils, a critical component of the innate immune response, during bacterial infection. We found that M-MDSCs, differentiated from monocytes in vitro, impaired neutrophil chemotaxis to IL-8 in a simple microfluidic chemotactic device. We then integrated M-MDSCs and neutrophils into our 3D infection-on-a-chip device that incorporates key features of an infectious environment including an endothelial lumen, a collagen extracellular matrix, and a source of Pseudomonas aeruginosa. When M-MDSCs were present in the matrix during simulated infection with Pseudomonas aeruginosa, significantly fewer neutrophils extravasated from the lumen, and those that left traveled a shorter distance from the lumen edge. We found IL-10 secretion increased during infections in the presence of M-MDSCs and blocking IL-10 restored neutrophil extravasation, indicating IL-10 secretion reduces neutrophil extravasation in the presence of M-MDSCs. In summary, we demonstrated impaired neutrophil chemotaxis, extravasation, and migration in the presence of M-MDSCs during bacterial infection and found increased levels of IL-10 contribute to reduced extravasation, indicating that MDSCs play a role in regulating the immune environment, leading to a reduced neutrophil response to infection.<\/p>\n<p>PMID:<a href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/41243824\/?utm_source=SimplePie&amp;utm_medium=rss&amp;utm_content=8405628&amp;ff=20251117094738&amp;v=2.18.0.post22+67771e2\">41243824<\/a> | DOI:<a href=\"https:\/\/doi.org\/10.1093\/jleuko\/qiaf165\">10.1093\/jleuko\/qiaf165<\/a><\/p>\n<\/div>","protected":false},"excerpt":{"rendered":"<p>J Leukoc Biol. 2025 Nov 17:qiaf165. doi: 10.1093\/jleuko\/qiaf165. Online ahead of print. ABSTRACT Patients who survive sepsis experience a prolonged period of immunosuppression. This period is accompanied by the expansion of monocytic myeloid-derived suppressor cells (M-MDSCs), a subset of suppressive myeloid cells; however, the impact of M-MDSCs on the innate immune response to infection is &#8230; <a title=\"Human M-MDSCs Impair Neutrophil Migration in the Infectious Microenvironment\u200bHannah K Weppner   on 17 de November de 2025 at 11:00\" class=\"read-more\" href=\"https:\/\/inmuno.es\/index.php\/2025\/11\/17\/human-m-mdscs-impair-neutrophil-migration-in-the-infectious-microenvironmenthannah-k-weppner-on-17-de-november-de-2025-at-1100\/\" aria-label=\"Read more about Human M-MDSCs Impair Neutrophil Migration in the Infectious Microenvironment\u200bHannah K Weppner   on 17 de November de 2025 at 11:00\">Read more<\/a><\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[86,42],"tags":[],"class_list":["post-48925","post","type-post","status-publish","format-standard","hentry","category-journal-of-leukocyte-biology","category-publicaciones"],"_links":{"self":[{"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/posts\/48925","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/comments?post=48925"}],"version-history":[{"count":0,"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/posts\/48925\/revisions"}],"wp:attachment":[{"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/media?parent=48925"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/categories?post=48925"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/tags?post=48925"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}