{"id":49333,"date":"2025-11-22T00:53:18","date_gmt":"2025-11-21T23:53:18","guid":{"rendered":"https:\/\/inmuno.es\/index.php\/2025\/11\/22\/tcpgdb-a-comprehensive-t-cell-perturbation-genomics-database-for-identification-of-critical-t-cell-regulators\/"},"modified":"2025-11-22T00:53:18","modified_gmt":"2025-11-21T23:53:18","slug":"tcpgdb-a-comprehensive-t-cell-perturbation-genomics-database-for-identification-of-critical-t-cell-regulators","status":"publish","type":"post","link":"https:\/\/inmuno.es\/index.php\/2025\/11\/22\/tcpgdb-a-comprehensive-t-cell-perturbation-genomics-database-for-identification-of-critical-t-cell-regulators\/","title":{"rendered":"TCPGdb: A comprehensive T cell perturbation genomics database for identification of critical T cell regulators"},"content":{"rendered":"<div>\n<p><b>Cancer Immunol Res<\/b>. 2025 Nov 21. doi: 10.1158\/2326-6066.CIR-25-0168. Online ahead of print.<\/p>\n<p><b>ABSTRACT<\/b><\/p>\n<p>Large parallel genetic screens have been used to identified targets and regulators that enhance T cell antitumor capability and persistence in tumor microenvironment. We hypothesized that by combining the pooled screen data from multiple independent genetic screens we could provide a systematic, comprehensive, and robust analysis of the effect of gene perturbation on T-cell based immunotherapies. After collecting data from previously published T cell screens, including CRISPR-based and ORF-based screens, through Gene Expression Omnibus (GEO), we reprocessed the gene hits summary and conducted a pathway enrichment analysis. A T cell screen perturbation score (TPS) metric was employed to quantify the impact of a gene perturbation on T cell function. Additionally, gene expression data (both bulk RNA level and single-cell RNA level) from autoimmune disease cohort and T cell-derived cancer patients were incorporated to gain further insight on gene perturbations that potentially augment T cell proliferation. We integrated all data and analysis on 35 T cells screens into our state-of-the-art T cell perturbation genomics database (TCPGdb), which is accessible through our webserver (http:\/\/tcpgdb.sidichenlab.org\/) and allows users to interactively explore the impact of query genes on T cell function.<\/p>\n<p>PMID:<a href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/41270225\/?utm_source=SimplePie&amp;utm_medium=rss&amp;utm_content=101614637&amp;ff=20251121185318&amp;v=2.18.0.post22+67771e2\">41270225<\/a> | DOI:<a href=\"https:\/\/doi.org\/10.1158\/2326-6066.CIR-25-0168\">10.1158\/2326-6066.CIR-25-0168<\/a><\/p>\n<\/div>","protected":false},"excerpt":{"rendered":"<p>Cancer Immunol Res. 2025 Nov 21. doi: 10.1158\/2326-6066.CIR-25-0168. Online ahead of print. ABSTRACT Large parallel genetic screens have been used to identified targets and regulators that enhance T cell antitumor capability and persistence in tumor microenvironment. We hypothesized that by combining the pooled screen data from multiple independent genetic screens we could provide a systematic, &#8230; <a title=\"TCPGdb: A comprehensive T cell perturbation genomics database for identification of critical T cell regulators\" class=\"read-more\" href=\"https:\/\/inmuno.es\/index.php\/2025\/11\/22\/tcpgdb-a-comprehensive-t-cell-perturbation-genomics-database-for-identification-of-critical-t-cell-regulators\/\" aria-label=\"Read more about TCPGdb: A comprehensive T cell perturbation genomics database for identification of critical T cell regulators\">Read more<\/a><\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[55,42],"tags":[],"class_list":["post-49333","post","type-post","status-publish","format-standard","hentry","category-cancer-immunology-reserch","category-publicaciones"],"_links":{"self":[{"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/posts\/49333","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/comments?post=49333"}],"version-history":[{"count":0,"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/posts\/49333\/revisions"}],"wp:attachment":[{"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/media?parent=49333"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/categories?post=49333"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/tags?post=49333"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}