{"id":50003,"date":"2025-12-02T13:41:07","date_gmt":"2025-12-02T12:41:07","guid":{"rendered":"https:\/\/inmuno.es\/index.php\/2025\/12\/02\/an-mrna-platform-to-create-isolated-monospecific-th1-responses\/"},"modified":"2025-12-02T13:41:07","modified_gmt":"2025-12-02T12:41:07","slug":"an-mrna-platform-to-create-isolated-monospecific-th1-responses","status":"publish","type":"post","link":"https:\/\/inmuno.es\/index.php\/2025\/12\/02\/an-mrna-platform-to-create-isolated-monospecific-th1-responses\/","title":{"rendered":"An mRNA platform to create isolated, monospecific Th1 responses"},"content":{"rendered":"<div>\n<p><b>J Immunol<\/b>. 2025 Dec 2:vkaf318. doi: 10.1093\/jimmun\/vkaf318. Online ahead of print.<\/p>\n<p><b>ABSTRACT<\/b><\/p>\n<p>Helper T cells (CD4 T cells) are lynchpins of adaptive immune responses. Each CD4 T cell expresses a single T-cell receptor, recognizing an epitope presented by major histocompatibility complex class II (MHC-II). Due to the enormous diversity of the T-cell repertoire, it is often desirable to study a population that responds to the same epitope. Here, we devised a novel method of selective immunization to produce a robust, monospecific helper T-cell response in mice using a modular mRNA vector. The vector encodes the target epitope attached via flexible linker to MHC-II. Immunization with this mRNA selectively elicits helper T-cell responses across a range of epitopes and MHC-II alleles. These CD4 T cells show robust, polyfunctional Th1 cytokine release when evaluated ex vivo. We tested the activity of these CD4 T cells in 2 disease models: Salmonella enterica and influenza virus. In both models, these monospecific CD4 T cells influenced the course of infection, demonstrating the utility of this experimental tool, which can produce a monospecific CD4 T-cell response.<\/p>\n<p>PMID:<a href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/41328008\/?utm_source=SimplePie&amp;utm_medium=rss&amp;utm_content=2985117R&amp;ff=20251202074106&amp;v=2.18.0.post22+67771e2\">41328008<\/a> | DOI:<a href=\"https:\/\/doi.org\/10.1093\/jimmun\/vkaf318\">10.1093\/jimmun\/vkaf318<\/a><\/p>\n<\/div>","protected":false},"excerpt":{"rendered":"<p>J Immunol. 2025 Dec 2:vkaf318. doi: 10.1093\/jimmun\/vkaf318. Online ahead of print. ABSTRACT Helper T cells (CD4 T cells) are lynchpins of adaptive immune responses. Each CD4 T cell expresses a single T-cell receptor, recognizing an epitope presented by major histocompatibility complex class II (MHC-II). Due to the enormous diversity of the T-cell repertoire, it is &#8230; <a title=\"An mRNA platform to create isolated, monospecific Th1 responses\" class=\"read-more\" href=\"https:\/\/inmuno.es\/index.php\/2025\/12\/02\/an-mrna-platform-to-create-isolated-monospecific-th1-responses\/\" aria-label=\"Read more about An mRNA platform to create isolated, monospecific Th1 responses\">Read more<\/a><\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[42,71],"tags":[],"class_list":["post-50003","post","type-post","status-publish","format-standard","hentry","category-publicaciones","category-the-journal-of-immunology"],"_links":{"self":[{"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/posts\/50003","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/comments?post=50003"}],"version-history":[{"count":0,"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/posts\/50003\/revisions"}],"wp:attachment":[{"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/media?parent=50003"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/categories?post=50003"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/tags?post=50003"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}