{"id":51162,"date":"2025-12-15T12:31:56","date_gmt":"2025-12-15T11:31:56","guid":{"rendered":"https:\/\/inmuno.es\/index.php\/2025\/12\/15\/single-cell-transcriptomic-analysis-reveals-rna-binding-protein-dysregulation-in-immune-checkpoint-inhibitor-induced-colitishaobei-ran-on-15-de-december-de-2025-at-1100\/"},"modified":"2025-12-15T12:31:56","modified_gmt":"2025-12-15T11:31:56","slug":"single-cell-transcriptomic-analysis-reveals-rna-binding-protein-dysregulation-in-immune-checkpoint-inhibitor-induced-colitishaobei-ran-on-15-de-december-de-2025-at-1100","status":"publish","type":"post","link":"https:\/\/inmuno.es\/index.php\/2025\/12\/15\/single-cell-transcriptomic-analysis-reveals-rna-binding-protein-dysregulation-in-immune-checkpoint-inhibitor-induced-colitishaobei-ran-on-15-de-december-de-2025-at-1100\/","title":{"rendered":"Single-Cell Transcriptomic Analysis Reveals RNA-Binding Protein Dysregulation in Immune Checkpoint Inhibitor-Induced Colitis\u200bHaobei Ran   on 15 de December de 2025 at 11:00"},"content":{"rendered":"<div>\n<p><b>J Leukoc Biol<\/b>. 2025 Dec 15:qiaf181. doi: 10.1093\/jleuko\/qiaf181. Online ahead of print.<\/p>\n<p><b>ABSTRACT<\/b><\/p>\n<p>Immune checkpoint inhibitors (ICIs) have revolutionized cancer therapy by enhancing anti-tumor immunity, but their use is frequently complicated by immune-related adverse events (irAEs), particularly ICI-induced diarrhea and colitis. Growing evidence suggests that RNA-binding proteins (RBPs), which are critical post-transcriptional regulators of immune responses, may play a pivotal role in inflammatory diseases. To systematically investigate the involvement of RBPs in ICI-induced colitis, we performed a comprehensive re-analysis of published single-cell RNA sequencing (scRNA-seq) data from CD45+ immune cells isolated from ICI colitis patients, ICI-treated non-colitis patients, and healthy controls. Our analysis revealed distinct, cell-type-specific RBP expression patterns across immune cell populations, with several RBP clusters (e.g., R4 in innate lymphoid cells (ILCs) and T cells, R5 in T cells, R8 in IgA+ plasma B cells, and R10 in B cells) being uniquely enriched in ICI colitis samples, suggesting their potential involvement in colitis pathogenesis. Further subpopulation analysis identified ICI colitis-deregulated RBPs: RPL26 was markedly downregulated in both ILCs and T cells from ICI colitis patients, while PCBP2, ISG20, HSPA1A and HSPA1B were upregulated in T cells. A colitis-specific RBP cluster was identified in B cells, among which ISG20 and ZFP36L2 showed elevated expression in specific subpopulations from colitis patients. ZFP36L2 potentially targets CD83 and JUNB, as their expression decreased in mouse germinal center B cells upon ZFP36L2 knockout. These findings highlight the regulatory role of RBPs in ICI-induced colitis and identify cell-type-specific alterations that may drive disease progression. Importantly, we pinpoint immune checkpoint-related RBPs as potential therapeutic targets, offering new strategies to manage this clinically significant irAE.<\/p>\n<p>PMID:<a href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/41392552\/?utm_source=SimplePie&amp;utm_medium=rss&amp;utm_content=8405628&amp;ff=20251215063156&amp;v=2.18.0.post22+67771e2\">41392552<\/a> | DOI:<a href=\"https:\/\/doi.org\/10.1093\/jleuko\/qiaf181\">10.1093\/jleuko\/qiaf181<\/a><\/p>\n<\/div>","protected":false},"excerpt":{"rendered":"<p>J Leukoc Biol. 2025 Dec 15:qiaf181. doi: 10.1093\/jleuko\/qiaf181. Online ahead of print. ABSTRACT Immune checkpoint inhibitors (ICIs) have revolutionized cancer therapy by enhancing anti-tumor immunity, but their use is frequently complicated by immune-related adverse events (irAEs), particularly ICI-induced diarrhea and colitis. Growing evidence suggests that RNA-binding proteins (RBPs), which are critical post-transcriptional regulators of immune &#8230; <a title=\"Single-Cell Transcriptomic Analysis Reveals RNA-Binding Protein Dysregulation in Immune Checkpoint Inhibitor-Induced Colitis\u200bHaobei Ran   on 15 de December de 2025 at 11:00\" class=\"read-more\" href=\"https:\/\/inmuno.es\/index.php\/2025\/12\/15\/single-cell-transcriptomic-analysis-reveals-rna-binding-protein-dysregulation-in-immune-checkpoint-inhibitor-induced-colitishaobei-ran-on-15-de-december-de-2025-at-1100\/\" aria-label=\"Read more about Single-Cell Transcriptomic Analysis Reveals RNA-Binding Protein Dysregulation in Immune Checkpoint Inhibitor-Induced Colitis\u200bHaobei Ran   on 15 de December de 2025 at 11:00\">Read more<\/a><\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[86,42],"tags":[],"class_list":["post-51162","post","type-post","status-publish","format-standard","hentry","category-journal-of-leukocyte-biology","category-publicaciones"],"_links":{"self":[{"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/posts\/51162","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/comments?post=51162"}],"version-history":[{"count":0,"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/posts\/51162\/revisions"}],"wp:attachment":[{"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/media?parent=51162"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/categories?post=51162"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/tags?post=51162"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}