{"id":51592,"date":"2025-12-20T09:59:55","date_gmt":"2025-12-20T08:59:55","guid":{"rendered":"https:\/\/inmuno.es\/index.php\/2025\/12\/20\/optimising-recovery-of-hepatic-regulatory-t-cells-a-practical-guide-using-artc2-blockade-valuecaitlin-abbott-violette-mouro-chiara-perucchini-chiara-vespari-matteo-iannacone\/"},"modified":"2025-12-20T09:59:55","modified_gmt":"2025-12-20T08:59:55","slug":"optimising-recovery-of-hepatic-regulatory-t-cells-a-practical-guide-using-artc2-blockade-valuecaitlin-abbott-violette-mouro-chiara-perucchini-chiara-vespari-matteo-iannacone","status":"publish","type":"post","link":"https:\/\/inmuno.es\/index.php\/2025\/12\/20\/optimising-recovery-of-hepatic-regulatory-t-cells-a-practical-guide-using-artc2-blockade-valuecaitlin-abbott-violette-mouro-chiara-perucchini-chiara-vespari-matteo-iannacone\/","title":{"rendered":"Optimising Recovery of Hepatic Regulatory T Cells: A Practical Guide Using ARTC2 Blockade. [[{&#8220;value&#8221;:&#8221;Caitlin Abbott, \nViolette Mouro, \nChiara Perucchini, \nChiara Vespari, \nMatteo Iannacone&#8221;}]]"},"content":{"rendered":"<p><img decoding=\"async\" src=\"https:\/\/onlinelibrary.wiley.com\/cms\/asset\/f7d5a859-7358-40c9-aca5-aea827c878f3\/eji70095-gra-0001-m.png\" alt=\"Optimising Recovery of Hepatic Regulatory T Cells: A Practical Guide Using ARTC2 Blockade\" \/><\/p>\n<p>This is an update to <a href=\"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/eji.202170126\">the Guidelines for the use of flow cytometry and cell sorting in immunological studies (third edition), Chapter 3: 12C, by Cossarizza et al<\/a>. Administration of anti-ARTC2 nanobody(S+16a) prevents cell death during tissue processing. We demonstrate that the phenotype of CD44<sup>mid<\/sup>Treg is significantly impacted, whereas the eTreg phenotype remains stable following S+16a treatment, outlining specific protocols for population recovery.<\/p>\n<p><\/p>","protected":false},"excerpt":{"rendered":"<p>This is an update to the Guidelines for the use of flow cytometry and cell sorting in immunological studies (third edition), Chapter 3: 12C, by Cossarizza et al. Administration of anti-ARTC2 nanobody(S+16a) prevents cell death during tissue processing. We demonstrate that the phenotype of CD44midTreg is significantly impacted, whereas the eTreg phenotype remains stable following &#8230; <a title=\"Optimising Recovery of Hepatic Regulatory T Cells: A Practical Guide Using ARTC2 Blockade. [[{&#8220;value&#8221;:&#8221;Caitlin Abbott, \nViolette Mouro, \nChiara Perucchini, \nChiara Vespari, \nMatteo Iannacone&#8221;}]]\" class=\"read-more\" href=\"https:\/\/inmuno.es\/index.php\/2025\/12\/20\/optimising-recovery-of-hepatic-regulatory-t-cells-a-practical-guide-using-artc2-blockade-valuecaitlin-abbott-violette-mouro-chiara-perucchini-chiara-vespari-matteo-iannacone\/\" aria-label=\"Read more about Optimising Recovery of Hepatic Regulatory T Cells: A Practical Guide Using ARTC2 Blockade. [[{&#8220;value&#8221;:&#8221;Caitlin Abbott, \nViolette Mouro, \nChiara Perucchini, \nChiara Vespari, \nMatteo Iannacone&#8221;}]]\">Read more<\/a><\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[112,42],"tags":[],"class_list":["post-51592","post","type-post","status-publish","format-standard","hentry","category-european-journal-of-immunology","category-publicaciones"],"_links":{"self":[{"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/posts\/51592","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/comments?post=51592"}],"version-history":[{"count":0,"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/posts\/51592\/revisions"}],"wp:attachment":[{"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/media?parent=51592"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/categories?post=51592"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/tags?post=51592"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}