{"id":51602,"date":"2025-12-21T10:31:42","date_gmt":"2025-12-21T09:31:42","guid":{"rendered":"https:\/\/inmuno.es\/index.php\/2025\/12\/21\/ccl21a-rather-than-ccl21b-is-essential-for-thymocyte-migration-in-mouse-valueizumi-ohigashi-hitomi-kyuma-eri-otsu-shinichi-hayashi-tatsuya-takemoto-yousuke-takahama\/"},"modified":"2025-12-21T10:31:42","modified_gmt":"2025-12-21T09:31:42","slug":"ccl21a-rather-than-ccl21b-is-essential-for-thymocyte-migration-in-mouse-valueizumi-ohigashi-hitomi-kyuma-eri-otsu-shinichi-hayashi-tatsuya-takemoto-yousuke-takahama","status":"publish","type":"post","link":"https:\/\/inmuno.es\/index.php\/2025\/12\/21\/ccl21a-rather-than-ccl21b-is-essential-for-thymocyte-migration-in-mouse-valueizumi-ohigashi-hitomi-kyuma-eri-otsu-shinichi-hayashi-tatsuya-takemoto-yousuke-takahama\/","title":{"rendered":"Ccl21a, Rather Than Ccl21b, is Essential for Thymocyte Migration in Mouse. [[{&#8220;value&#8221;:&#8221;Izumi Ohigashi, \nHitomi Kyuma, \nEri Otsu, \nShinichi Hayashi, \nTatsuya Takemoto, \nYousuke Takahama&#8221;}]]"},"content":{"rendered":"<p><img decoding=\"async\" src=\"https:\/\/onlinelibrary.wiley.com\/cms\/asset\/52fc5683-65ae-495d-b86a-3488a5b949a7\/eji70114-gra-0001-m.png\" alt=\"Ccl21a, Rather Than Ccl21b, is Essential for Thymocyte Migration in Mouse\" \/><\/p>\n<p>CCR7-mediated chemokine signaling regulates the medullary migration of thymocytes to establish self-tolerance. By comparing the thymic phenotype of <i>Ccl21b<\/i>-KO mice with that of <i>Ccl21a<\/i>-KO mice, we show that <i>Ccl21a<\/i> plays a predominant role compared with <i>Ccl21b<\/i> among CCR7 ligands in the thymus.<\/p>\n<p><\/p>\n<h2>ABSTRACT<\/h2>\n<p>Self-tolerance in T cells is a vital self-defense strategy for mammals to specifically respond to invading pathogens. During T cell development in the thymus, thymocytes migrate from the cortex to the medulla to sequentially acquire non-self-reactivity and self-tolerance. This cortex-to-medulla migration is regulated by CCR7-mediated chemokine signaling. Previous studies have identified CCL21 but not CCL19 as a functional ligand for this CCR7-dependent migration. CCL21 in the mouse is encoded by multiple genes, including CCL21Ser-encoding <i>Ccl21a<\/i> and several CCL21Leu-encoding genes, including <i>Ccl21b<\/i>. The importance of <i>Ccl21a<\/i> in thymocyte migration has been demonstrated, whereas the role of CCL21Leu-encoding genes remains unclear. By producing mice specifically deficient in <i>Ccl21b<\/i>, we show that <i>Ccl21b<\/i> plays little to no role in the cortex-to-medulla migration of developing thymocytes. CCL21Leu-encoding gene transcripts remain detectable even in the absence of <i>Ccl21b<\/i>, suggesting that <i>Ccl21b<\/i> is not a major source of CCL21Leu. We further show that the copy number of CCL21Leu-encoding genes is smaller than the currently estimated copy number in a public database. These findings underscore the predominant role of <i>Ccl21a<\/i> over <i>Ccl21b<\/i> in the mouse thymus.<\/p>","protected":false},"excerpt":{"rendered":"<p>CCR7-mediated chemokine signaling regulates the medullary migration of thymocytes to establish self-tolerance. By comparing the thymic phenotype of Ccl21b-KO mice with that of Ccl21a-KO mice, we show that Ccl21a plays a predominant role compared with Ccl21b among CCR7 ligands in the thymus. ABSTRACT Self-tolerance in T cells is a vital self-defense strategy for mammals to &#8230; <a title=\"Ccl21a, Rather Than Ccl21b, is Essential for Thymocyte Migration in Mouse. [[{&#8220;value&#8221;:&#8221;Izumi Ohigashi, \nHitomi Kyuma, \nEri Otsu, \nShinichi Hayashi, \nTatsuya Takemoto, \nYousuke Takahama&#8221;}]]\" class=\"read-more\" href=\"https:\/\/inmuno.es\/index.php\/2025\/12\/21\/ccl21a-rather-than-ccl21b-is-essential-for-thymocyte-migration-in-mouse-valueizumi-ohigashi-hitomi-kyuma-eri-otsu-shinichi-hayashi-tatsuya-takemoto-yousuke-takahama\/\" aria-label=\"Read more about Ccl21a, Rather Than Ccl21b, is Essential for Thymocyte Migration in Mouse. [[{&#8220;value&#8221;:&#8221;Izumi Ohigashi, \nHitomi Kyuma, \nEri Otsu, \nShinichi Hayashi, \nTatsuya Takemoto, \nYousuke Takahama&#8221;}]]\">Read more<\/a><\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[112,42],"tags":[],"class_list":["post-51602","post","type-post","status-publish","format-standard","hentry","category-european-journal-of-immunology","category-publicaciones"],"_links":{"self":[{"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/posts\/51602","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/comments?post=51602"}],"version-history":[{"count":0,"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/posts\/51602\/revisions"}],"wp:attachment":[{"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/media?parent=51602"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/categories?post=51602"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/tags?post=51602"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}