{"id":52648,"date":"2026-01-12T08:18:20","date_gmt":"2026-01-12T07:18:20","guid":{"rendered":"https:\/\/inmuno.es\/index.php\/2026\/01\/12\/integrated-human-and-mouse-single-cell-profiling-reveals-immune-stromal-niche-driving-silicosis-christina-begka\/"},"modified":"2026-01-12T08:18:20","modified_gmt":"2026-01-12T07:18:20","slug":"integrated-human-and-mouse-single-cell-profiling-reveals-immune-stromal-niche-driving-silicosis-christina-begka","status":"publish","type":"post","link":"https:\/\/inmuno.es\/index.php\/2026\/01\/12\/integrated-human-and-mouse-single-cell-profiling-reveals-immune-stromal-niche-driving-silicosis-christina-begka\/","title":{"rendered":"Integrated human and mouse single-cell profiling reveals immune-stromal niche driving silicosis. Christina Begka"},"content":{"rendered":"<div>\n<p><b>Mucosal Immunol<\/b>. 2026 Jan 9:S1933-0219(26)00003-6. doi: 10.1016\/j.mucimm.2026.01.003. Online ahead of print.<\/p>\n<p><b>ABSTRACT<\/b><\/p>\n<p>Silicosis is an inflammation-driven pulmonary fibrosis caused by occupational inhalation of silica particles. Macrophages are crucial in silicosis pathology, yet their interaction with stromal cells in orchestrating fibrosis progression remains poorly understood. Single-cell RNA sequencing (scRNAseq) of whole-lung lavages from silicosis patients identified the expansion of an intermediate CCL2-hi monocyte-like macrophage (MLM) cluster that further differentiated into inflammatory IL1B-hi and pre-fibrotic SPP1-hi (osteopontin) subsets. SPP1-hi MLMs showed enrichment for tissue remodelling (SPP1, CHI3L1, MMP14, COL6A1), oxidative stress (GCLC, TXN, PRDX1), and bio-mineralisation genes (GLA, CA2, CTSK). To explore immune-stromal dynamics, we developed a site-specific silicosis mouse model via mini-bronchoscopy. Mouse scRNAseq analysis and cell-cell communication modelling identified novel neutrophil subsets, reprogrammed alveolar type 2 epithelial cells, and two Sfrp1-hi\/Spp1-hi fibroblast subsets involved in extensive crosstalk with MLMs. These data identify key components of the silicotic niche and predict targetable interactions within the immune-stromal axis for ameliorating disease.<\/p>\n<p>PMID:<a href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/41520918\/?utm_source=SimplePie&amp;utm_medium=rss&amp;utm_content=101299742&amp;ff=20260112021819&amp;v=2.18.0.post22+67771e2\">41520918<\/a> | DOI:<a href=\"https:\/\/doi.org\/10.1016\/j.mucimm.2026.01.003\">10.1016\/j.mucimm.2026.01.003<\/a><\/p>\n<\/div>","protected":false},"excerpt":{"rendered":"<p>Mucosal Immunol. 2026 Jan 9:S1933-0219(26)00003-6. doi: 10.1016\/j.mucimm.2026.01.003. Online ahead of print. ABSTRACT Silicosis is an inflammation-driven pulmonary fibrosis caused by occupational inhalation of silica particles. Macrophages are crucial in silicosis pathology, yet their interaction with stromal cells in orchestrating fibrosis progression remains poorly understood. Single-cell RNA sequencing (scRNAseq) of whole-lung lavages from silicosis patients identified &#8230; <a title=\"Integrated human and mouse single-cell profiling reveals immune-stromal niche driving silicosis. Christina Begka\" class=\"read-more\" href=\"https:\/\/inmuno.es\/index.php\/2026\/01\/12\/integrated-human-and-mouse-single-cell-profiling-reveals-immune-stromal-niche-driving-silicosis-christina-begka\/\" aria-label=\"Read more about Integrated human and mouse single-cell profiling reveals immune-stromal niche driving silicosis. Christina Begka\">Read more<\/a><\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[57,42],"tags":[],"class_list":["post-52648","post","type-post","status-publish","format-standard","hentry","category-mucosal-immunology","category-publicaciones"],"_links":{"self":[{"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/posts\/52648","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/comments?post=52648"}],"version-history":[{"count":0,"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/posts\/52648\/revisions"}],"wp:attachment":[{"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/media?parent=52648"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/categories?post=52648"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/tags?post=52648"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}