{"id":56482,"date":"2026-02-18T00:51:38","date_gmt":"2026-02-17T23:51:38","guid":{"rendered":"https:\/\/inmuno.es\/index.php\/2026\/02\/18\/rabep1-regulates-neutrophil-migration-via-endosomal-recycling-and-actin-polymerizationdaniel-h-kim-on-17-de-february-de-2026-at-1100\/"},"modified":"2026-02-18T00:51:38","modified_gmt":"2026-02-17T23:51:38","slug":"rabep1-regulates-neutrophil-migration-via-endosomal-recycling-and-actin-polymerizationdaniel-h-kim-on-17-de-february-de-2026-at-1100","status":"publish","type":"post","link":"https:\/\/inmuno.es\/index.php\/2026\/02\/18\/rabep1-regulates-neutrophil-migration-via-endosomal-recycling-and-actin-polymerizationdaniel-h-kim-on-17-de-february-de-2026-at-1100\/","title":{"rendered":"RABEP1 regulates neutrophil migration via endosomal recycling and actin polymerization\u200bDaniel H Kim   on 17 de February de 2026 at 11:00"},"content":{"rendered":"<div>\n<p><b>J Leukoc Biol<\/b>. 2026 Feb 9;118(2):qiaf174. doi: 10.1093\/jleuko\/qiaf174.<\/p>\n<p><b>ABSTRACT<\/b><\/p>\n<p>Neutrophils are the first responders of our innate immune system, crucial for defense against various infections. The intricate regulation of neutrophil migration is essential for neutrophil function. However, a complete mechanistic understanding is missing. We previously performed a miRNA overexpression screen and identified miR-190 as a potent suppressor of neutrophil migration in zebrafish. Through a second round of small-scale screening using neutrophil-specific knockouts of putative miR-190 targets, we identified that rabep1 (encoding Rabaptin, RAB GTPase binding effector protein 1) is essential for neutrophil motility and chemotaxis in zebrafish. Re-expressing full-length Rabaptin, but not its truncation lacking the Rab4\/Rab5 binding domain, rescued cell motility in the knockout. Knocking down RABEP1 in differentiated human leukemia (dHL-60) cells consistently reduced cell motility. RABAPTIN-deficient dHL-60 cells are defective with fast recycling, yet maintain a normal Rab5 GTP level. The RABAPTIN-deficient cells displayed reduced PAK phosphorylation and decreased F-actin levels, yet still appropriately polarized upon chemokine stimulation. Overexpression of dominant-negative Rab4 or Rab5 has a similar inhibitory effect on neutrophil migration. Our data suggest that RABAPTIN drives endosomal recycling, Rac activation, and leading-edge actin polymerization, providing significant insights into the role of the endocytic pathway in neutrophil motility.<\/p>\n<p>PMID:<a href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/41701563\/?utm_source=SimplePie&amp;utm_medium=rss&amp;utm_content=8405628&amp;ff=20260217185131&amp;v=2.18.0.post22+67771e2\">41701563<\/a> | DOI:<a href=\"https:\/\/doi.org\/10.1093\/jleuko\/qiaf174\">10.1093\/jleuko\/qiaf174<\/a><\/p>\n<\/div>","protected":false},"excerpt":{"rendered":"<p>J Leukoc Biol. 2026 Feb 9;118(2):qiaf174. doi: 10.1093\/jleuko\/qiaf174. ABSTRACT Neutrophils are the first responders of our innate immune system, crucial for defense against various infections. The intricate regulation of neutrophil migration is essential for neutrophil function. However, a complete mechanistic understanding is missing. We previously performed a miRNA overexpression screen and identified miR-190 as a &#8230; <a title=\"RABEP1 regulates neutrophil migration via endosomal recycling and actin polymerization\u200bDaniel H Kim   on 17 de February de 2026 at 11:00\" class=\"read-more\" href=\"https:\/\/inmuno.es\/index.php\/2026\/02\/18\/rabep1-regulates-neutrophil-migration-via-endosomal-recycling-and-actin-polymerizationdaniel-h-kim-on-17-de-february-de-2026-at-1100\/\" aria-label=\"Read more about RABEP1 regulates neutrophil migration via endosomal recycling and actin polymerization\u200bDaniel H Kim   on 17 de February de 2026 at 11:00\">Read more<\/a><\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[86,42],"tags":[],"class_list":["post-56482","post","type-post","status-publish","format-standard","hentry","category-journal-of-leukocyte-biology","category-publicaciones"],"_links":{"self":[{"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/posts\/56482","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/comments?post=56482"}],"version-history":[{"count":0,"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/posts\/56482\/revisions"}],"wp:attachment":[{"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/media?parent=56482"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/categories?post=56482"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/tags?post=56482"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}