{"id":56802,"date":"2026-02-23T19:04:57","date_gmt":"2026-02-23T18:04:57","guid":{"rendered":"https:\/\/inmuno.es\/index.php\/2026\/02\/23\/a-multitherapy-single-cell-atlas-reveals-cell-type-specific-modulation-in-sepsis-induced-liver-injury\/"},"modified":"2026-02-23T19:04:57","modified_gmt":"2026-02-23T18:04:57","slug":"a-multitherapy-single-cell-atlas-reveals-cell-type-specific-modulation-in-sepsis-induced-liver-injury","status":"publish","type":"post","link":"https:\/\/inmuno.es\/index.php\/2026\/02\/23\/a-multitherapy-single-cell-atlas-reveals-cell-type-specific-modulation-in-sepsis-induced-liver-injury\/","title":{"rendered":"A multitherapy single-cell atlas reveals cell type-specific modulation in sepsis-induced liver injury"},"content":{"rendered":"<div>\n<p><b>J Immunol<\/b>. 2026 Feb 9;215(2):vkaf351. doi: 10.1093\/jimmun\/vkaf351.<\/p>\n<p><b>ABSTRACT<\/b><\/p>\n<p>Sepsis-induced liver injury involves profound immune dysregulation. Natural compounds such as artesunate (ART), capsaicin (CAP), and oridonin (ORI) have demonstrated efficacy in mitigating systemic inflammation; however, their comparative cellular mechanisms in sepsis remain poorly characterized. Here, we integrated and reanalyzed the single-cell transcriptomic datasets of murine livers from 5 conditions: healthy control, sepsis, and sepsis treated with ART, CAP, or ORI. We uncover a spectrum of neutrophil subtypes with treatment-responsive phenotypes, including anti-inflammatory Ngp+ Neu1, immunosuppressive Cd274+ Neu2, and mature Stfa2l1+ Neu4, in which the excessive neutrophil expansion was suppressed by all 3 therapies through distinct regulon activities. Macrophages were activated and infiltration to partially rebalance immune homeostasis. Endothelial cells underwent profound reprogramming under sepsis, marked by NF-\u03baB activation and oxidative stress, which are selectively modulated by treatment. Cell-cell communication analysis revealed a convergent dampening of inflammatory ligand-receptor networks, including the CCL signaling axis, and therapy-specific enhancement of regenerative cues, such as EGF signaling. Our findings reveal both shared and compound-specific immunoregulatory effects of ART, CAP, and ORI, offering mechanistic insights into hepatic immune rebalancing in sepsis. This single-cell atlas provides a conceptual framework for the rational design of multitarget therapies and highlights the key immune modules amenable to therapeutic intervention.<\/p>\n<p>PMID:<a href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/41729163\/?utm_source=SimplePie&amp;utm_medium=rss&amp;utm_content=2985117R&amp;ff=20260223130449&amp;v=2.18.0.post22+67771e2\">41729163<\/a> | DOI:<a href=\"https:\/\/doi.org\/10.1093\/jimmun\/vkaf351\">10.1093\/jimmun\/vkaf351<\/a><\/p>\n<\/div>","protected":false},"excerpt":{"rendered":"<p>J Immunol. 2026 Feb 9;215(2):vkaf351. doi: 10.1093\/jimmun\/vkaf351. ABSTRACT Sepsis-induced liver injury involves profound immune dysregulation. Natural compounds such as artesunate (ART), capsaicin (CAP), and oridonin (ORI) have demonstrated efficacy in mitigating systemic inflammation; however, their comparative cellular mechanisms in sepsis remain poorly characterized. Here, we integrated and reanalyzed the single-cell transcriptomic datasets of murine livers &#8230; <a title=\"A multitherapy single-cell atlas reveals cell type-specific modulation in sepsis-induced liver injury\" class=\"read-more\" href=\"https:\/\/inmuno.es\/index.php\/2026\/02\/23\/a-multitherapy-single-cell-atlas-reveals-cell-type-specific-modulation-in-sepsis-induced-liver-injury\/\" aria-label=\"Read more about A multitherapy single-cell atlas reveals cell type-specific modulation in sepsis-induced liver injury\">Read more<\/a><\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[42,71],"tags":[],"class_list":["post-56802","post","type-post","status-publish","format-standard","hentry","category-publicaciones","category-the-journal-of-immunology"],"_links":{"self":[{"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/posts\/56802","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/comments?post=56802"}],"version-history":[{"count":0,"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/posts\/56802\/revisions"}],"wp:attachment":[{"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/media?parent=56802"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/categories?post=56802"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/tags?post=56802"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}