{"id":57017,"date":"2026-02-25T09:35:29","date_gmt":"2026-02-25T08:35:29","guid":{"rendered":"https:\/\/inmuno.es\/index.php\/2026\/02\/25\/spatial-profiling-of-germinal-centers-in-mouse-secondary-lymphoid-organs-using-macsima-imaging-cyclic-staining-technology-valueisabel-san-martin-molina-hanneke-okkenhaug-simon-walker\/"},"modified":"2026-02-25T09:35:29","modified_gmt":"2026-02-25T08:35:29","slug":"spatial-profiling-of-germinal-centers-in-mouse-secondary-lymphoid-organs-using-macsima-imaging-cyclic-staining-technology-valueisabel-san-martin-molina-hanneke-okkenhaug-simon-walker","status":"publish","type":"post","link":"https:\/\/inmuno.es\/index.php\/2026\/02\/25\/spatial-profiling-of-germinal-centers-in-mouse-secondary-lymphoid-organs-using-macsima-imaging-cyclic-staining-technology-valueisabel-san-martin-molina-hanneke-okkenhaug-simon-walker\/","title":{"rendered":"Spatial Profiling of Germinal Centers in Mouse Secondary Lymphoid Organs Using MACSima Imaging Cyclic Staining Technology. [[{&#8220;value&#8221;:&#8221;Isabel San Mart\u00edn Molina, \nHanneke Okkenhaug, \nSimon Walker, \nMichelle Linterman, \nEdith Marcial\u2010Ju\u00e1rez&#8221;}]]"},"content":{"rendered":"<p><img decoding=\"async\" src=\"https:\/\/onlinelibrary.wiley.com\/cms\/asset\/02722af6-f554-4627-9f9a-f997301a3923\/eji70157-gra-0001-m.png\" alt=\"Spatial Profiling of Germinal Centers in Mouse Secondary Lymphoid Organs Using MACSima Imaging Cyclic Staining Technology\" \/><\/p>\n<p>We developed a 50-plex imaging protocol using the MACSima platform to characterize the microarchitecture of germinal centers in secondary lymphoid tissues. This workflow combines tissue processing, automated cyclic imaging, image preprocessing, and a new analysis method that detects morphological changes in irregularly shaped stromal cells.<\/p>\n<p><\/p>","protected":false},"excerpt":{"rendered":"<p>We developed a 50-plex imaging protocol using the MACSima platform to characterize the microarchitecture of germinal centers in secondary lymphoid tissues. This workflow combines tissue processing, automated cyclic imaging, image preprocessing, and a new analysis method that detects morphological changes in irregularly shaped stromal cells.<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[112,42],"tags":[],"class_list":["post-57017","post","type-post","status-publish","format-standard","hentry","category-european-journal-of-immunology","category-publicaciones"],"_links":{"self":[{"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/posts\/57017","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/comments?post=57017"}],"version-history":[{"count":0,"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/posts\/57017\/revisions"}],"wp:attachment":[{"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/media?parent=57017"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/categories?post=57017"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/tags?post=57017"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}