{"id":61025,"date":"2026-04-14T07:19:09","date_gmt":"2026-04-14T05:19:09","guid":{"rendered":"https:\/\/inmuno.es\/index.php\/2026\/04\/14\/effectiveness-and-immunogenicity-of-a-nanoemulsion-protein-subunit-vaccine-against-pseudomonas-aeruginosa-investigation-in-diet-induced-obese-mice\/"},"modified":"2026-04-14T07:19:09","modified_gmt":"2026-04-14T05:19:09","slug":"effectiveness-and-immunogenicity-of-a-nanoemulsion-protein-subunit-vaccine-against-pseudomonas-aeruginosa-investigation-in-diet-induced-obese-mice","status":"publish","type":"post","link":"https:\/\/inmuno.es\/index.php\/2026\/04\/14\/effectiveness-and-immunogenicity-of-a-nanoemulsion-protein-subunit-vaccine-against-pseudomonas-aeruginosa-investigation-in-diet-induced-obese-mice\/","title":{"rendered":"Effectiveness and immunogenicity of a nanoemulsion protein subunit vaccine against Pseudomonas aeruginosa: investigation in diet-induced obese mice"},"content":{"rendered":"<div>\n<p><b>J Immunol<\/b>. 2026 Mar 17;215(3):vkag052. doi: 10.1093\/jimmun\/vkag052.<\/p>\n<p><b>ABSTRACT<\/b><\/p>\n<p>Pseudomonas aeruginosa (Pa) is an opportunistic pathogen threatening individuals with obesity, a condition associated with chronic meta-inflammation and altered immunity. In this study, we evaluate the immunogenicity and protective efficacy of a nanoemulsion-based subunit vaccine (L-PaF\/ME\/BECC) targeting Pa in a diet-induced obese mouse model. Mice fed a high-fat diet (HFD; 60% fat) were compared to those on a low-fat diet (LFD; 10% fat) or regular chow (3% to 4% fat) to assess differences in vaccine responsiveness. Our results show that while L-PaF induces robust immune responses across all groups, HFD-fed mice exhibit significantly impaired antibody quality, including reduced IgA, IgG1, and IgG3 titers. These deficiencies were associated with impaired germinal center B-cell and T follicular helper cell responses, along with reduced CXCR5 and PD-1 modulation. In contrast, LFD-fed mice displayed preserved germinal center architecture and enhanced class-switched antibody production. This immune impairment extended to the lung-resident memory B cell in HFD mice, whereas LFD mice maintained a functional response. Despite compromised humoral immunity, HFD mice showed an expansion of lung tissue-resident memory T (Trm) cells postvaccination. FTY720 treatment showed that early secondary protection against Pa was maintained in the absence of circulating T-cell recruitment, consistent with a dominant contribution of lung-resident immune mechanisms during this early recall phase. Together, these findings suggest that lung Trm-associated immunity may partially compensate for obesity-associated defects in humoral responses and highlight the importance of vaccine strategies that promote tissue-localized T-cell immunity in metabolically compromised hosts.<\/p>\n<p>PMID:<a href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/41974204\/?utm_source=SimplePie&amp;utm_medium=rss&amp;utm_content=2985117R&amp;ff=20260414011908&amp;v=2.19.0.post6+133c1fe\">41974204<\/a> | DOI:<a href=\"https:\/\/doi.org\/10.1093\/jimmun\/vkag052\">10.1093\/jimmun\/vkag052<\/a><\/p>\n<\/div>","protected":false},"excerpt":{"rendered":"<p>J Immunol. 2026 Mar 17;215(3):vkag052. doi: 10.1093\/jimmun\/vkag052. ABSTRACT Pseudomonas aeruginosa (Pa) is an opportunistic pathogen threatening individuals with obesity, a condition associated with chronic meta-inflammation and altered immunity. In this study, we evaluate the immunogenicity and protective efficacy of a nanoemulsion-based subunit vaccine (L-PaF\/ME\/BECC) targeting Pa in a diet-induced obese mouse model. Mice fed a &#8230; <a title=\"Effectiveness and immunogenicity of a nanoemulsion protein subunit vaccine against Pseudomonas aeruginosa: investigation in diet-induced obese mice\" class=\"read-more\" href=\"https:\/\/inmuno.es\/index.php\/2026\/04\/14\/effectiveness-and-immunogenicity-of-a-nanoemulsion-protein-subunit-vaccine-against-pseudomonas-aeruginosa-investigation-in-diet-induced-obese-mice\/\" aria-label=\"Read more about Effectiveness and immunogenicity of a nanoemulsion protein subunit vaccine against Pseudomonas aeruginosa: investigation in diet-induced obese mice\">Read more<\/a><\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[42,71],"tags":[],"class_list":["post-61025","post","type-post","status-publish","format-standard","hentry","category-publicaciones","category-the-journal-of-immunology"],"_links":{"self":[{"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/posts\/61025","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/comments?post=61025"}],"version-history":[{"count":0,"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/posts\/61025\/revisions"}],"wp:attachment":[{"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/media?parent=61025"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/categories?post=61025"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/tags?post=61025"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}