{"id":62123,"date":"2026-04-20T19:23:26","date_gmt":"2026-04-20T17:23:26","guid":{"rendered":"https:\/\/inmuno.es\/index.php\/2026\/04\/20\/transcriptional-programming-of-early-forming-memory-b-cells-arises-independently-of-cognate-cd4-t-cell-interactions\/"},"modified":"2026-04-20T19:23:26","modified_gmt":"2026-04-20T17:23:26","slug":"transcriptional-programming-of-early-forming-memory-b-cells-arises-independently-of-cognate-cd4-t-cell-interactions","status":"publish","type":"post","link":"https:\/\/inmuno.es\/index.php\/2026\/04\/20\/transcriptional-programming-of-early-forming-memory-b-cells-arises-independently-of-cognate-cd4-t-cell-interactions\/","title":{"rendered":"Transcriptional programming of early-forming memory B cells arises independently of cognate CD4+ T-cell interactions"},"content":{"rendered":"<div>\n<p><b>J Immunol<\/b>. 2026 Apr 15;215(4):vkag054. doi: 10.1093\/jimmun\/vkag054.<\/p>\n<p><b>ABSTRACT<\/b><\/p>\n<p>Memory B cells (MBCs) are an integral part of the humoral immune response with the capacity to both reseed germinal center reactions and rapidly form antibody-secreting plasma cells (ASCs) upon secondary antigen encounter. MBCs arise via both T cell-dependent and -independent routes and while CD4+ T cells are not required for their formation, it is still not clear if or how initial T:B-cell interactions influence the molecular programming and diversity of T cell-independent MBCs. To address this, we characterized MBCs that form in response to influenza infection in major histocompatibility complex class II knockout (MHCII-KO) mice, which lack CD4+ T cells. Consistent with T cell-independent responses, the MBCs that formed in MHCII-KO mice were reduced in number and did not acquire surface expression of CCR6 and class-switched BCR. Transcriptional profiling identified cytokine- and activation-induced genes that were reduced in expression in MHCII-KO MBCs compared to wild type (WT). Adoptive transfer of MHCII-KO B cells into WT hosts, which cannot receive peptide\/MHCII-TCR cognate interactions, revealed an ability of MHCII-KO cells to form MBCs and ASCs. Single-cell RNA sequencing revealed minimal transcriptional differences between MHCII-KO and WT MBCs, indicating that cognate CD4+ T-cell interactions provide limited early programming instruction to MBCs. MHCII-KO MBCs were able to clonally expand, class-switch, and form the same diverse transcriptional clusters as WT. These data indicate that early differentiating MBCs can seed a diverse pool of MBC populations in response to influenza infection independent of cognate T-cell help.<\/p>\n<p>PMID:<a href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/42001515\/?utm_source=SimplePie&amp;utm_medium=rss&amp;utm_content=2985117R&amp;ff=20260420132324&amp;v=2.19.0.post6+133c1fe\">42001515<\/a> | DOI:<a href=\"https:\/\/doi.org\/10.1093\/jimmun\/vkag054\">10.1093\/jimmun\/vkag054<\/a><\/p>\n<\/div>","protected":false},"excerpt":{"rendered":"<p>J Immunol. 2026 Apr 15;215(4):vkag054. doi: 10.1093\/jimmun\/vkag054. ABSTRACT Memory B cells (MBCs) are an integral part of the humoral immune response with the capacity to both reseed germinal center reactions and rapidly form antibody-secreting plasma cells (ASCs) upon secondary antigen encounter. MBCs arise via both T cell-dependent and -independent routes and while CD4+ T cells &#8230; <a title=\"Transcriptional programming of early-forming memory B cells arises independently of cognate CD4+ T-cell interactions\" class=\"read-more\" href=\"https:\/\/inmuno.es\/index.php\/2026\/04\/20\/transcriptional-programming-of-early-forming-memory-b-cells-arises-independently-of-cognate-cd4-t-cell-interactions\/\" aria-label=\"Read more about Transcriptional programming of early-forming memory B cells arises independently of cognate CD4+ T-cell interactions\">Read more<\/a><\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[42,71],"tags":[],"class_list":["post-62123","post","type-post","status-publish","format-standard","hentry","category-publicaciones","category-the-journal-of-immunology"],"_links":{"self":[{"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/posts\/62123","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/comments?post=62123"}],"version-history":[{"count":0,"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/posts\/62123\/revisions"}],"wp:attachment":[{"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/media?parent=62123"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/categories?post=62123"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/tags?post=62123"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}