{"id":62316,"date":"2026-04-22T13:03:45","date_gmt":"2026-04-22T11:03:45","guid":{"rendered":"https:\/\/inmuno.es\/index.php\/2026\/04\/22\/cd19-expression-associates-with-polyfunctionality-and-altered-secretion-dynamics-in-individual-activated-human-b-cells\/"},"modified":"2026-04-22T13:03:45","modified_gmt":"2026-04-22T11:03:45","slug":"cd19-expression-associates-with-polyfunctionality-and-altered-secretion-dynamics-in-individual-activated-human-b-cells","status":"publish","type":"post","link":"https:\/\/inmuno.es\/index.php\/2026\/04\/22\/cd19-expression-associates-with-polyfunctionality-and-altered-secretion-dynamics-in-individual-activated-human-b-cells\/","title":{"rendered":"CD19 expression associates with polyfunctionality and altered secretion dynamics in individual activated human B cells"},"content":{"rendered":"<div>\n<p><b>J Immunol<\/b>. 2026 Apr 15;215(4):vkag063. doi: 10.1093\/jimmun\/vkag063.<\/p>\n<p><b>ABSTRACT<\/b><\/p>\n<p>The concept of polyfunctional secretion, the ability of cells to secrete multiple, functionally distinct proteins, has received increased attention for B cells as they have been described to secrete both antibodies and cytokines. However, the functional analysis of B cells was mostly performed in bulk, and consequently, the dynamics, quantity, and temporality of poly-secreting B cells are not well understood on the level of the individual cell. Furthermore, data on the existence of B-cell polyfunctionality in healthy settings remain scarce. Therefore, in this work, we developed a microfluidic workflow for the quantitative, simultaneous analysis of B-cell polyfunctionality on the single-cell level, focusing on 4 key secreted proteins-IL-10, IL-6, GM-CSF, and IgG-in switched memory B cells after ex vivo stimulation. Our findings revealed that IgG cytokine co-secretion occurred at the single-cell level, and was induced early by specific stimuli. Notably, ex vivo TLR4 activation of human B cells induced a population of highly polyfunctional B cells, wherein IL-6 and GM-CSF exhibited faster secretion dynamics compared to their mono-secreting counterparts. Moreover, these functional differences correlated with CD19 expression: Higher CD19 levels resulted in increased IL-6 and GM-CSF secretion rates and delayed IgG secretion. This suggests distinct functional properties correlating with CD19 expression levels in ex vivo activated antibody-secreting and B cells. Beyond the quantitative and dynamic description of inflammatory and regulatory B cells, these results offer insights into the complexity and temporal patterns of B-cell (poly)functionalities, opening new avenues for studying disease-related immune regulation.<\/p>\n<p>PMID:<a href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/42015530\/?utm_source=SimplePie&amp;utm_medium=rss&amp;utm_content=2985117R&amp;ff=20260422070345&amp;v=2.19.0.post6+133c1fe\">42015530<\/a> | DOI:<a href=\"https:\/\/doi.org\/10.1093\/jimmun\/vkag063\">10.1093\/jimmun\/vkag063<\/a><\/p>\n<\/div>","protected":false},"excerpt":{"rendered":"<p>J Immunol. 2026 Apr 15;215(4):vkag063. doi: 10.1093\/jimmun\/vkag063. ABSTRACT The concept of polyfunctional secretion, the ability of cells to secrete multiple, functionally distinct proteins, has received increased attention for B cells as they have been described to secrete both antibodies and cytokines. However, the functional analysis of B cells was mostly performed in bulk, and consequently, &#8230; <a title=\"CD19 expression associates with polyfunctionality and altered secretion dynamics in individual activated human B cells\" class=\"read-more\" href=\"https:\/\/inmuno.es\/index.php\/2026\/04\/22\/cd19-expression-associates-with-polyfunctionality-and-altered-secretion-dynamics-in-individual-activated-human-b-cells\/\" aria-label=\"Read more about CD19 expression associates with polyfunctionality and altered secretion dynamics in individual activated human B cells\">Read more<\/a><\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[42,71],"tags":[],"class_list":["post-62316","post","type-post","status-publish","format-standard","hentry","category-publicaciones","category-the-journal-of-immunology"],"_links":{"self":[{"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/posts\/62316","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/comments?post=62316"}],"version-history":[{"count":0,"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/posts\/62316\/revisions"}],"wp:attachment":[{"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/media?parent=62316"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/categories?post=62316"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/tags?post=62316"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}