{"id":62317,"date":"2026-04-22T13:03:46","date_gmt":"2026-04-22T11:03:46","guid":{"rendered":"https:\/\/inmuno.es\/index.php\/2026\/04\/22\/immunological-evaluation-of-vaccines-to-prevent-chlamydia-trachomatis-infection-using-the-human-in-vitro-mimic-system\/"},"modified":"2026-04-22T13:03:46","modified_gmt":"2026-04-22T11:03:46","slug":"immunological-evaluation-of-vaccines-to-prevent-chlamydia-trachomatis-infection-using-the-human-in-vitro-mimic-system","status":"publish","type":"post","link":"https:\/\/inmuno.es\/index.php\/2026\/04\/22\/immunological-evaluation-of-vaccines-to-prevent-chlamydia-trachomatis-infection-using-the-human-in-vitro-mimic-system\/","title":{"rendered":"Immunological evaluation of vaccines to prevent Chlamydia trachomatis infection using the human in vitro MIMIC system"},"content":{"rendered":"<div>\n<p><b>J Immunol<\/b>. 2026 Apr 15;215(4):vkag080. doi: 10.1093\/jimmun\/vkag080.<\/p>\n<p><b>ABSTRACT<\/b><\/p>\n<p>The Modular Immune In vitro Construct (MIMIC) system is an established laboratory-based methodology that reproduces in vitro the human in vivo T-cell immune response. In this study, we utilized the MIMIC system to establish a dendritic cell and CD4+ T-cell co-culture model that recapitulates T helper cell responses to Chlamydia trachomatis infection in humans. We demonstrate that C. trachomatis infection induces C. trachomatis-specific IFN-\u03b3-producing CD4+ T-cell responses, with IFN-\u03b3 as the dominant cytokine, accompanied by significant production of TNF-\u03b1, IL-13, IL-8, and IL-6. Live C. trachomatis induces stronger IFN-\u03b3 CD4+ T-cell responses than heat-killed bacteria. A C. trachomatis outer membrane protein vaccine comprising the major outer membrane protein and polymorphic membrane proteins PmpE, PmpG, and PmpH elicited limited CD4+ T-cell responses, while an attenuated Chlamydia muridarum whole cell-based vaccine (intrOv) generated robust CD4+ T-cell responses against C. trachomatis challenge, encompassing multiple genital C. trachomatis serovars. The results suggest that intrOv vaccine should advance along the clinical development pathway as a chlamydia vaccine. MIMIC represents a valuable platform for evaluating chlamydia vaccine candidates, and it can reduce time and cost required to advance vaccine candidates to clinical trials.<\/p>\n<p>PMID:<a href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/42015529\/?utm_source=SimplePie&amp;utm_medium=rss&amp;utm_content=2985117R&amp;ff=20260422070345&amp;v=2.19.0.post6+133c1fe\">42015529<\/a> | DOI:<a href=\"https:\/\/doi.org\/10.1093\/jimmun\/vkag080\">10.1093\/jimmun\/vkag080<\/a><\/p>\n<\/div>","protected":false},"excerpt":{"rendered":"<p>J Immunol. 2026 Apr 15;215(4):vkag080. doi: 10.1093\/jimmun\/vkag080. ABSTRACT The Modular Immune In vitro Construct (MIMIC) system is an established laboratory-based methodology that reproduces in vitro the human in vivo T-cell immune response. In this study, we utilized the MIMIC system to establish a dendritic cell and CD4+ T-cell co-culture model that recapitulates T helper cell &#8230; <a title=\"Immunological evaluation of vaccines to prevent Chlamydia trachomatis infection using the human in vitro MIMIC system\" class=\"read-more\" href=\"https:\/\/inmuno.es\/index.php\/2026\/04\/22\/immunological-evaluation-of-vaccines-to-prevent-chlamydia-trachomatis-infection-using-the-human-in-vitro-mimic-system\/\" aria-label=\"Read more about Immunological evaluation of vaccines to prevent Chlamydia trachomatis infection using the human in vitro MIMIC system\">Read more<\/a><\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[42,71],"tags":[],"class_list":["post-62317","post","type-post","status-publish","format-standard","hentry","category-publicaciones","category-the-journal-of-immunology"],"_links":{"self":[{"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/posts\/62317","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/comments?post=62317"}],"version-history":[{"count":0,"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/posts\/62317\/revisions"}],"wp:attachment":[{"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/media?parent=62317"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/categories?post=62317"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/tags?post=62317"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}