{"id":63504,"date":"2026-05-07T13:06:12","date_gmt":"2026-05-07T11:06:12","guid":{"rendered":"https:\/\/inmuno.es\/index.php\/2026\/05\/07\/glut1-mediated-glucose-flux-constrains-autoreactive-but-not-foreign-antigen-specific-b-cell-responses\/"},"modified":"2026-05-07T13:06:12","modified_gmt":"2026-05-07T11:06:12","slug":"glut1-mediated-glucose-flux-constrains-autoreactive-but-not-foreign-antigen-specific-b-cell-responses","status":"publish","type":"post","link":"https:\/\/inmuno.es\/index.php\/2026\/05\/07\/glut1-mediated-glucose-flux-constrains-autoreactive-but-not-foreign-antigen-specific-b-cell-responses\/","title":{"rendered":"GLUT1-mediated glucose flux constrains autoreactive but not foreign antigen-specific B-cell responses"},"content":{"rendered":"<div>\n<p><b>J Immunol<\/b>. 2026 Apr 15;215(4):vkag092. doi: 10.1093\/jimmun\/vkag092.<\/p>\n<p><b>ABSTRACT<\/b><\/p>\n<p>Emerging evidence from lupus-prone mice and patients with systemic lupus erythematosus implicates enhanced glycolysis in lymphocytes as a driver of disease. We previously showed that the pharmacologic blockade of glycolysis reduced the production of autoantibodies without affecting antibodies induced by immunization to a foreign protein. Here we used CRISPR\/Cas9 to reduce the expression of glucose transporter GLUT1 in B cells from autoreactive AM14 Vk8R (AM14) and antigen-specific B1-8 J\u03ba (B1-8) transgenic mice, comparing intrinsic glycolytic requirements across disease-relevant contexts. Following adoptive transfer into BALB\/c recipients, Glut1 knockdown (Glut1KD) decreased the persistence of AM14 B cells, their differentiation into plasmablasts, and production of antibodies upon immunization with the PL2-3 hybridoma that activates both their B-cell receptor and endosomal TLR. In addition, PL2-3-stimulated Glut1KD AM14 B cells selectively reduced their CD80 expression both in vivo and in vitro, as well as ATP production and mammalian target of rapamycin (mTOR) signaling in vitro. In contrast, Glut1KD B1-8 B cells retained persistence, plasmablast output, and nitrophenyl (NP)-specific IgM production after NP-OVA immunization, with a selective reduction in the proliferation of naive B cells. Bioenergetic output was preserved despite Glut1KD in both clones stimulated with TLR7 agonist R848, but CD80 and mTOR signaling were differentially affected. Thus, GLUT1-dependent glycolysis is essential for immune complex-driven autoreactive B-cell activation yet largely dispensable for antigen-specific responses, identifying metabolic checkpoints that may selectively restrain pathogenic B cells while sparing protective humoral immunity.<\/p>\n<p>PMID:<a href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/42092338\/?utm_source=SimplePie&amp;utm_medium=rss&amp;utm_content=2985117R&amp;ff=20260507070612&amp;v=2.19.0.post6+133c1fe\">42092338<\/a> | DOI:<a href=\"https:\/\/doi.org\/10.1093\/jimmun\/vkag092\">10.1093\/jimmun\/vkag092<\/a><\/p>\n<\/div>","protected":false},"excerpt":{"rendered":"<p>J Immunol. 2026 Apr 15;215(4):vkag092. doi: 10.1093\/jimmun\/vkag092. ABSTRACT Emerging evidence from lupus-prone mice and patients with systemic lupus erythematosus implicates enhanced glycolysis in lymphocytes as a driver of disease. We previously showed that the pharmacologic blockade of glycolysis reduced the production of autoantibodies without affecting antibodies induced by immunization to a foreign protein. Here we &#8230; <a title=\"GLUT1-mediated glucose flux constrains autoreactive but not foreign antigen-specific B-cell responses\" class=\"read-more\" href=\"https:\/\/inmuno.es\/index.php\/2026\/05\/07\/glut1-mediated-glucose-flux-constrains-autoreactive-but-not-foreign-antigen-specific-b-cell-responses\/\" aria-label=\"Read more about GLUT1-mediated glucose flux constrains autoreactive but not foreign antigen-specific B-cell responses\">Read more<\/a><\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[42,71],"tags":[],"class_list":["post-63504","post","type-post","status-publish","format-standard","hentry","category-publicaciones","category-the-journal-of-immunology"],"_links":{"self":[{"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/posts\/63504","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/comments?post=63504"}],"version-history":[{"count":0,"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/posts\/63504\/revisions"}],"wp:attachment":[{"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/media?parent=63504"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/categories?post=63504"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/tags?post=63504"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}