{"id":63505,"date":"2026-05-07T13:06:13","date_gmt":"2026-05-07T11:06:13","guid":{"rendered":"https:\/\/inmuno.es\/index.php\/2026\/05\/07\/role-of-ca2-activated-kca3-1-potassium-channel-in-car-t-cell-effector-function\/"},"modified":"2026-05-07T13:06:13","modified_gmt":"2026-05-07T11:06:13","slug":"role-of-ca2-activated-kca3-1-potassium-channel-in-car-t-cell-effector-function","status":"publish","type":"post","link":"https:\/\/inmuno.es\/index.php\/2026\/05\/07\/role-of-ca2-activated-kca3-1-potassium-channel-in-car-t-cell-effector-function\/","title":{"rendered":"Role of Ca2+-activated KCa3.1 potassium channel in CAR T cell effector function"},"content":{"rendered":"<div>\n<p><b>J Immunol<\/b>. 2026 Apr 15;215(4):vkag078. doi: 10.1093\/jimmun\/vkag078.<\/p>\n<p><b>ABSTRACT<\/b><\/p>\n<p>Genetically modified chimeric antigen receptor (CAR) T cells eliminate tumors by recognizing specific antigens on the cell surface. T cell ion channels (e.g. Kv1.3, KCa3.1) influence activation, proliferation, and effector functions such as target cell killing, through the regulation of Ca2+ signaling. We showed that CAR-expressing cells (Jurkat) specifically eliminate tumor cells (Raji B cells) in monolayer culture and inhibition of KCa3.1 by TRAM34 increased the tumor cell-killing ability of KCa3.1+ Jurkat CARs. Blockage of KCa3.1 facilitated the migration of KCa3.1+ Jurkat CARs (mean speed, displacement and distance). The application of TRAM34 lowered the baseline Ca2+ level in mCherry-KCa3.1+ Jurkat CARs. Finally, TRAM34 significantly reduced the time needed to eliminate tumor cells. We concluded that expression and modification of KCa3.1 ion channels shifts the intracellular Ca2+ concentration into the range where cytotoxicity dominates. Hence, modification of KCa3.1 channels could contribute to a more effective anticancer immunotherapy.<\/p>\n<p>PMID:<a href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/42092337\/?utm_source=SimplePie&amp;utm_medium=rss&amp;utm_content=2985117R&amp;ff=20260507070612&amp;v=2.19.0.post6+133c1fe\">42092337<\/a> | DOI:<a href=\"https:\/\/doi.org\/10.1093\/jimmun\/vkag078\">10.1093\/jimmun\/vkag078<\/a><\/p>\n<\/div>","protected":false},"excerpt":{"rendered":"<p>J Immunol. 2026 Apr 15;215(4):vkag078. doi: 10.1093\/jimmun\/vkag078. ABSTRACT Genetically modified chimeric antigen receptor (CAR) T cells eliminate tumors by recognizing specific antigens on the cell surface. T cell ion channels (e.g. Kv1.3, KCa3.1) influence activation, proliferation, and effector functions such as target cell killing, through the regulation of Ca2+ signaling. We showed that CAR-expressing cells &#8230; <a title=\"Role of Ca2+-activated KCa3.1 potassium channel in CAR T cell effector function\" class=\"read-more\" href=\"https:\/\/inmuno.es\/index.php\/2026\/05\/07\/role-of-ca2-activated-kca3-1-potassium-channel-in-car-t-cell-effector-function\/\" aria-label=\"Read more about Role of Ca2+-activated KCa3.1 potassium channel in CAR T cell effector function\">Read more<\/a><\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[42,71],"tags":[],"class_list":["post-63505","post","type-post","status-publish","format-standard","hentry","category-publicaciones","category-the-journal-of-immunology"],"_links":{"self":[{"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/posts\/63505","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/comments?post=63505"}],"version-history":[{"count":0,"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/posts\/63505\/revisions"}],"wp:attachment":[{"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/media?parent=63505"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/categories?post=63505"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/tags?post=63505"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}