{"id":67065,"date":"2026-06-10T13:39:41","date_gmt":"2026-06-10T11:39:41","guid":{"rendered":"https:\/\/inmuno.es\/index.php\/2026\/06\/10\/homozygous-kinase-dead-csf1r-mutation-in-outbred-mice-reveals-essential-and-redundant-functions-of-tissue-resident-macrophagessebastien-jacquelin-on-10-de-june-de-2026-at-1000\/"},"modified":"2026-06-10T13:39:41","modified_gmt":"2026-06-10T11:39:41","slug":"homozygous-kinase-dead-csf1r-mutation-in-outbred-mice-reveals-essential-and-redundant-functions-of-tissue-resident-macrophagessebastien-jacquelin-on-10-de-june-de-2026-at-1000","status":"publish","type":"post","link":"https:\/\/inmuno.es\/index.php\/2026\/06\/10\/homozygous-kinase-dead-csf1r-mutation-in-outbred-mice-reveals-essential-and-redundant-functions-of-tissue-resident-macrophagessebastien-jacquelin-on-10-de-june-de-2026-at-1000\/","title":{"rendered":"Homozygous kinase-dead Csf1r mutation in outbred mice reveals essential and redundant functions of tissue resident macrophages\u200bSebastien Jacquelin   on 10 de June de 2026 at 10:00"},"content":{"rendered":"<div>\n<p><b>J Leukoc Biol<\/b>. 2026 Jun 10:qiag074. doi: 10.1093\/jleuko\/qiag074. Online ahead of print.<\/p>\n<p><b>ABSTRACT<\/b><\/p>\n<p>The proliferation, differentiation and survival of cells of the macrophage lineage depends on signals from the macrophage colony-stimulating factor receptor (CSF1R). On a C57BL\/6J background homozygous kinase-dead Csf1r mutation (Csf1rE631K\/E631K &#8211; E631Km\/m) causes perinatal lethality. By contrast, E631Km\/m mice on a mixed genetic background (C57 x BALB\/c) were osteoclast-deficient\/osteopetrotic and growth retarded but did not develop hydrocephalus or other major structural brain abnormalities seen in inbred Csf1r-\/- mice and were viable as adults. Outbred E631Km\/m mice lacked tissue resident macrophages detected with a Csf1r-EGFP transgene or F4\/80 in most major organs including the brain but aside from reproductive organs, which were under-developed, no gross histological abnormalities were observed. E631Km\/m genotype was associated with reduced blood monocytes, the loss of circulating C1Q, reduced IGF1 and increased CSF1. In mice, F4\/80+\/CD169+ resident marrow macrophages are believed to be essential components of erythroblastic and hematopoietic island niches. These cells were undetectable in E631Km\/m marrow. Their absence was associated with granulocytosis and B cell deficiency in marrow, blood and spleen whereas the relative abundance of pluripotent and committed progenitors was not affected. Erythropoietic homeostasis was also maintained, erythroblasts were physically associated with a minor residual population of CSF1R-independent CD169-ve\/F4\/80+ marrow macrophages. We conclude that many developmental and homeostatic functions attributed to mouse resident tissue macrophages are redundant, species-specific or restricted to inbred strains.<\/p>\n<p>PMID:<a href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/42266149\/?utm_source=SimplePie&amp;utm_medium=rss&amp;utm_content=8405628&amp;ff=20260610073940&amp;v=2.20.0\">42266149<\/a> | DOI:<a href=\"https:\/\/doi.org\/10.1093\/jleuko\/qiag074\">10.1093\/jleuko\/qiag074<\/a><\/p>\n<\/div>","protected":false},"excerpt":{"rendered":"<p>J Leukoc Biol. 2026 Jun 10:qiag074. doi: 10.1093\/jleuko\/qiag074. Online ahead of print. ABSTRACT The proliferation, differentiation and survival of cells of the macrophage lineage depends on signals from the macrophage colony-stimulating factor receptor (CSF1R). On a C57BL\/6J background homozygous kinase-dead Csf1r mutation (Csf1rE631K\/E631K &#8211; E631Km\/m) causes perinatal lethality. By contrast, E631Km\/m mice on a mixed &#8230; <a title=\"Homozygous kinase-dead Csf1r mutation in outbred mice reveals essential and redundant functions of tissue resident macrophages\u200bSebastien Jacquelin   on 10 de June de 2026 at 10:00\" class=\"read-more\" href=\"https:\/\/inmuno.es\/index.php\/2026\/06\/10\/homozygous-kinase-dead-csf1r-mutation-in-outbred-mice-reveals-essential-and-redundant-functions-of-tissue-resident-macrophagessebastien-jacquelin-on-10-de-june-de-2026-at-1000\/\" aria-label=\"Read more about Homozygous kinase-dead Csf1r mutation in outbred mice reveals essential and redundant functions of tissue resident macrophages\u200bSebastien Jacquelin   on 10 de June de 2026 at 10:00\">Read more<\/a><\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[86,42],"tags":[],"class_list":["post-67065","post","type-post","status-publish","format-standard","hentry","category-journal-of-leukocyte-biology","category-publicaciones"],"_links":{"self":[{"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/posts\/67065","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/comments?post=67065"}],"version-history":[{"count":0,"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/posts\/67065\/revisions"}],"wp:attachment":[{"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/media?parent=67065"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/categories?post=67065"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/tags?post=67065"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}